Attenuation of photobleaching in two-photon excitation fluorescence from green fluorescent protein with shaped excitation pulses

2003 ◽  
Vol 311 (3) ◽  
pp. 592-596 ◽  
Author(s):  
Hiroyuki Kawano ◽  
Yasuo Nabekawa ◽  
Akira Suda ◽  
Yu Oishi ◽  
Hideaki Mizuno ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Two Photon ◽  
Photon Excitation ◽  
Green Fluorescent ◽  
Two Photon Excitation

High-Order Photobleaching of Green Fluorescent Protein inside Live Cells in Two-Photon Excitation Microscopy

2002 ◽  
Vol 291 (5) ◽  
pp. 1272-1275 ◽  
Author(s):  
Tong-Sheng Chen ◽  
Shao-Qun Zeng ◽  
Qing-Ming Luo ◽  
Zhi-Hong Zhang ◽  
Wei Zhou
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
High Order ◽  
Live Cells ◽  
Two Photon ◽  
Photon Excitation ◽  
Green Fluorescent ◽  
Two Photon Excitation

A Comparison of the Fluorescence Dynamics of Single Molecules of a Green Fluorescent Protein: One- versus Two-Photon Excitation

ChemPhysChem ◽  
2006 ◽  
Vol 7 (1) ◽  
pp. 250-260 ◽  
Author(s):  
Mircea Cotlet ◽  
Peter M. Goodwin ◽  
Geoffrey S. Waldo ◽  
James H. Werner
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Single Molecules ◽  
Two Photon ◽  
Photon Excitation ◽  
Green Fluorescent ◽  
Two Photon Excitation

Two-photon excitation and emission spectra of the green fluorescent protein variants ECFP, EGFP and EYFP

2005 ◽  
Vol 217 (3) ◽  
pp. 200-204 ◽  
Author(s):  
E. SPIESS ◽  
F. BESTVATER ◽  
A. HECKEL-POMPEY ◽  
K. TOTH ◽  
M. HACKER ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Emission Spectra ◽  
Two Photon ◽  
Photon Excitation ◽  
Green Fluorescent ◽  
Protein Variants ◽  
Two Photon Excitation

scholarly journals Illuminating the Formation of Lumens

2007 ◽  
Vol 15 (3) ◽  
pp. 3-5
Author(s):  
Stephen W. Carmichael
Keyword(s):  
Animal Model ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Solid Core ◽  
Red Fluorescent Protein ◽  
Neuronal Process ◽  
Two Photon ◽  
Green Fluorescent

How do lumens form? Two mechanisms that come readily to mind are a wrapping model, similar to the wrapping of the myelin sheath around a neuronal process, and a solid core of cells followed by apoptosis of the central cells. Another obvious mechanism that was suggested over 100 years ago is the fusion of intracellular vacuoles. Whereas several recent studies have supported this latter mechanism, it has not yet been proven. Now, the appropriate animal model (zebrafish), the modern techniques (transgenic chimeras), dyes (green fluorescent protein and monomeric red fluorescent protein) that can be linked to proteins to label vacuoles, and two-photon imaging in real time finally have provided the strongest support yet. In an article by Makoto Kamei, Brian Saunders, Kayla Bayless, Louis Dye, George Davis, and Brant Weinstein the assembly of endothelial tubes from intracellular vacuoles was observed in vitro and in vivo.

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