scholarly journals Blue-Shifted Green Fluorescent Protein Homologues Are Brighter than Enhanced Green Fluorescent Protein under Two-Photon Excitation

2017 ◽  
Vol 8 (12) ◽  
pp. 2548-2554 ◽  
Author(s):  
Rosana S. Molina ◽  
Tam M. Tran ◽  
Robert E. Campbell ◽  
Gerard G. Lambert ◽  
Anya Salih ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Two Photon ◽  
Photon Excitation ◽  
Green Fluorescent ◽  
Two Photon Excitation

High-Order Photobleaching of Green Fluorescent Protein inside Live Cells in Two-Photon Excitation Microscopy

2002 ◽  
Vol 291 (5) ◽  
pp. 1272-1275 ◽  
Author(s):  
Tong-Sheng Chen ◽  
Shao-Qun Zeng ◽  
Qing-Ming Luo ◽  
Zhi-Hong Zhang ◽  
Wei Zhou
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
High Order ◽  
Live Cells ◽  
Two Photon ◽  
Photon Excitation ◽  
Green Fluorescent ◽  
Two Photon Excitation

A Comparison of the Fluorescence Dynamics of Single Molecules of a Green Fluorescent Protein: One- versus Two-Photon Excitation

ChemPhysChem ◽  
2006 ◽  
Vol 7 (1) ◽  
pp. 250-260 ◽  
Author(s):  
Mircea Cotlet ◽  
Peter M. Goodwin ◽  
Geoffrey S. Waldo ◽  
James H. Werner
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Single Molecules ◽  
Two Photon ◽  
Photon Excitation ◽  
Green Fluorescent ◽  
Two Photon Excitation

Attenuation of photobleaching in two-photon excitation fluorescence from green fluorescent protein with shaped excitation pulses

2003 ◽  
Vol 311 (3) ◽  
pp. 592-596 ◽  
Author(s):  
Hiroyuki Kawano ◽  
Yasuo Nabekawa ◽  
Akira Suda ◽  
Yu Oishi ◽  
Hideaki Mizuno ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Two Photon ◽  
Photon Excitation ◽  
Green Fluorescent ◽  
Two Photon Excitation

Two-photon excitation and emission spectra of the green fluorescent protein variants ECFP, EGFP and EYFP

2005 ◽  
Vol 217 (3) ◽  
pp. 200-204 ◽  
Author(s):  
E. SPIESS ◽  
F. BESTVATER ◽  
A. HECKEL-POMPEY ◽  
K. TOTH ◽  
M. HACKER ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Emission Spectra ◽  
Two Photon ◽  
Photon Excitation ◽  
Green Fluorescent ◽  
Protein Variants ◽  
Two Photon Excitation

scholarly journals Enhanced Green Fluorescent Protein (GFP) fluorescence after polyelectrolyte caging

2006 ◽  
Vol 14 (21) ◽  
pp. 9815 ◽  
Author(s):  
Alberto Diaspro ◽  
Silke Krol ◽  
Barbara Campanini ◽  
Fabio Cannone ◽  
Giuseppe Chirico
Keyword(s):  
Green Fluorescent Protein ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Gfp Fluorescence ◽  
Green Fluorescent

scholarly journals Development and Characterization of a cDNA-Launch Recombinant Simian Hemorrhagic Fever Virus Expressing Enhanced Green Fluorescent Protein: ORF 2b’ Is Not Required for In Vitro Virus Replication

Viruses ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 632
Author(s):  
Yingyun Cai ◽  
Shuiqing Yu ◽  
Ying Fang ◽  
Laura Bollinger ◽  
Yanhua Li ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Cell Lines ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Infectious Clone ◽  
Hemorrhagic Fever ◽  
Simian Hemorrhagic Fever Virus ◽  
Hemorrhagic Fever Virus ◽  
Green Fluorescent

Simian hemorrhagic fever virus (SHFV) causes acute, lethal disease in macaques. We developed a single-plasmid cDNA-launch infectious clone of SHFV (rSHFV) and modified the clone to rescue an enhanced green fluorescent protein-expressing rSHFV-eGFP that can be used for rapid and quantitative detection of infection. SHFV has a narrow cell tropism in vitro, with only the grivet MA-104 cell line and a few other grivet cell lines being susceptible to virion entry and permissive to infection. Using rSHFV-eGFP, we demonstrate that one cricetid rodent cell line and three ape cell lines also fully support SHFV replication, whereas 55 human cell lines, 11 bat cell lines, and three rodent cells do not. Interestingly, some human and other mammalian cell lines apparently resistant to SHFV infection are permissive after transfection with the rSHFV-eGFP cDNA-launch plasmid. To further demonstrate the investigative potential of the infectious clone system, we introduced stop codons into eight viral open reading frames (ORFs). This approach suggested that at least one ORF, ORF 2b’, is dispensable for SHFV in vitro replication. Our proof-of-principle experiments indicated that rSHFV-eGFP is a useful tool for illuminating the understudied molecular biology of SHFV.

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