Incorporation of biodegradable nanoparticles into human airway epithelium cells—in vitro study of the suitability as a vehicle for drug or gene delivery in pulmonary diseases

2004 ◽  
Vol 318 (2) ◽  
pp. 562-570 ◽  
Author(s):  
M Brzoska ◽  
K Langer ◽  
C Coester ◽  
S Loitsch ◽  
T.O.F Wagner ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Airway Epithelium ◽  
In Vitro Study ◽  
Pulmonary Diseases ◽  
Biodegradable Nanoparticles ◽  
Human Airway ◽  
Human Airway Epithelium ◽  
Or Gene ◽  
Epithelium Cells

scholarly journals An approach to testing undiluted e-cigarette aerosol in vitro using 3D reconstituted human airway epithelium

2019 ◽  
Vol 54 ◽  
pp. 391-401 ◽  
Author(s):  
E. Bishop ◽  
L. Haswell ◽  
J. Adamson ◽  
S. Costigan ◽  
D. Thorne ◽  
...  
Keyword(s):  
Airway Epithelium ◽  
Human Airway ◽  
Human Airway Epithelium

An in vitro model of human airway epithelium (EpiAirway) for in vitro metabolism and toxicity screening

2007 ◽  
Vol 172 ◽  
pp. S79 ◽  
Author(s):  
Patrick Hayden ◽  
Joseph Kubilus ◽  
Helena Kandárová ◽  
Mitchell Klausner ◽  
George Jackson ◽  
...  
Keyword(s):  
Airway Epithelium ◽  
In Vitro Model ◽  
In Vitro Metabolism ◽  
Toxicity Screening ◽  
Human Airway ◽  
Human Airway Epithelium ◽  
Vitro Model

scholarly journals In vitro differentiation of ciliated cells in ALI-cultured human airway epithelium – the framework for functional studies on airway differentiation in ciliopathies

2021 ◽  
pp. 151189
Author(s):  
Zuzanna Bukowy-Bieryłło ◽  
Patrycja Daca-Roszak ◽  
Joanna Jurczak ◽  
Hanna Przystałowska-Macioła ◽  
Roman Jaksik ◽  
...  
Keyword(s):  
Airway Epithelium ◽  
Ciliated Cells ◽  
In Vitro Differentiation ◽  
Functional Studies ◽  
Human Airway ◽  
Human Airway Epithelium

scholarly journals DAS181, a sialidase fusion protein, protects human airway epithelium against influenza virus infection: an in vitro pharmacodynamic analysis

2009 ◽  
Vol 65 (2) ◽  
pp. 275-284 ◽  
Author(s):  
Gallen B. Triana-Baltzer ◽  
Maria Babizki ◽  
Michael C. W. Chan ◽  
Adam C. N. Wong ◽  
Laura M. Aschenbrenner ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Virus Infection ◽  
Fusion Protein ◽  
Airway Epithelium ◽  
Influenza Virus Infection ◽  
Human Airway ◽  
Human Airway Epithelium ◽  
Pharmacodynamic Analysis

scholarly journals Glucocorticoid Clearance and Metabolite Profiling in an In Vitro Human Airway Epithelium Lung Model

2015 ◽  
Vol 44 (2) ◽  
pp. 220-226 ◽  
Author(s):  
D. Rivera-Burgos ◽  
U. Sarkar ◽  
A. R. Lever ◽  
M. J. Avram ◽  
J. R. Coppeta ◽  
...  
Keyword(s):  
Airway Epithelium ◽  
Metabolite Profiling ◽  
Lung Model ◽  
Human Airway ◽  
Human Airway Epithelium

scholarly journals Toxicological Assessment of ITER-Like Tungsten Nanoparticles Using an In Vitro 3D Human Airway Epithelium Model

Nanomaterials ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 1374
Author(s):  
Isabelle George ◽  
Chiara Uboldi ◽  
Elodie Bernard ◽  
Marcos Sobrido ◽  
Sarah Dine ◽  
...  
Keyword(s):  
Metabolic Activity ◽  
Airway Epithelium ◽  
Cell Model ◽  
Experimental Conditions ◽  
Toxicological Assessment ◽  
Human Airway ◽  
Human Airway Epithelium ◽  
A Minor ◽  
Tungsten Nanoparticles

The International Thermonuclear Experimental Reactor (ITER) is an international project aimed at the production of carbon-free energy through the use of thermonuclear fusion. During ITER operation, in case of a loss-of-vacuum-accident, tungsten nanoparticles (W-NPs) could potentially be released into the environment and induce occupational exposure via inhalation. W-NPs toxicity was evaluated on MucilAir™, a 3D in vitro cell model of the human airway epithelium. MucilAir™ was exposed for 24 h to metallic ITER-like milled W-NPs, tungstate (WO42−) and tungsten carbide cobalt particles alloy (WC-Co). Cytotoxicity and its reversibility were assessed using a kinetic mode up to 28 days after exposure. Epithelial tightness, metabolic activity and interleukin-8 release were also evaluated. Electron microscopy was performed to determine any morphological modification, while mass spectrometry allowed the quantification of W-NPs internalization and of W transfer through the MucilAir™. Our results underlined a decrease in barrier integrity, no effect on metabolic activity or cell viability and a transient increase in IL-8 secretion after exposure to ITER-like milled W-NPs. These effects were associated with W-transfer through the epithelium, but not with intracellular accumulation. We have shown that, under our experimental conditions, ITER-like milled W-NPs have a minor impact on the MucilAir™ in vitro model.

scholarly journals Rhinovirus C replication is associated with the endoplasmic reticulum and triggers cytopathic effects in an in vitro model of human airway epithelium

2021 ◽  
Author(s):  
Talita B Gagliardi ◽  
Monty E Goldstein ◽  
Daniel Song ◽  
Kelsey M Gray ◽  
Jae W Jung ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Airway Epithelium ◽  
In Vitro Model ◽  
Cytopathic Effects ◽  
Human Airway ◽  
Human Airway Epithelium ◽  
Vitro Model ◽  
Rhinovirus C ◽  
The Impact

The clinical impact of rhinovirus C (RV-C) is well-documented; yet the viral life cycle remains poorly defined. Thus, we characterized RV-C15 replication at the single-cell level and its impact on the human airway epithelium (HAE) using a physiologically-relevant in vitro model. RV-C15 replication was restricted to ciliated cells where viral RNA levels peaked at 12 hours post-infection (hpi), correlating with elevated titers in the apical compartment at 24 hpi. Notably, infection was associated with a loss of polarized expression of the RV-C receptor, cadherin-related family member 3. Visualization of double-stranded RNA (dsRNA) during RV-C15 replication revealed two distinct replication complex arrangements within the cell, likely corresponding to different time points in infection and correlating with the formation of large intracellular vesicles. To further define RV-C15 replication sites, we analyzed the expression of giantin, phosphatidylinositol-4-phosphate, and calnexin, as well as the colocalization of these markers with dsRNA. Fluorescence levels of all three cellular markers were elevated during infection and altered giantin distribution further indicated Golgi fragmentation. However, unlike previously characterized RVs, the high ratio of calnexin-dsRNA colocalization implicated the endoplasmic reticulum as the primary site for RV-C15 replication in HAE. RV-C15 infection was also associated with elevated stimulator of interferon genes (STING) expression, facilitating replication, and the induction of incomplete autophagy, a mechanism used by other RVs to promote non-lytic release of progeny virions. Finally, RV-C15 infection resulted in a temporary loss in epithelial barrier integrity and the translocation of tight junction proteins while a reduction in mucociliary clearance indicated cytopathic effects on epithelial function. Together, our findings identify both shared and unique features of RV-C replication compared to related rhinoviruses and define the impact of RV-C on both epithelial cell organization and tissue functionality - aspects of infection that may contribute to pathogenesis in vivo.

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