Functional analysis, overexpression, and kinetic characterization of pyruvate kinase from Plasmodium falciparum

2004 ◽  
Vol 326 (1) ◽  
pp. 188-196 ◽  
Author(s):  
Maurice Chan ◽  
Tiow-Suan Sim
Keyword(s):  
Functional Analysis ◽  
Plasmodium Falciparum ◽  
Pyruvate Kinase ◽  
Kinetic Characterization

Functional Analysis, Overexpression, and Kinetic Characterization of Pyruvate Kinase from Methicillin-ResistantStaphylococcus aureus

Biochemistry ◽  
2010 ◽  
Vol 49 (35) ◽  
pp. 7733-7747 ◽  
Author(s):  
Roya Zoraghi ◽  
Raymond H. See ◽  
Huansheng Gong ◽  
Tian Lian ◽  
Rick Swayze ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Pyruvate Kinase ◽  
Kinetic Characterization

scholarly journals Structural and kinetic characterization of Trypanosoma congolense pyruvate kinase

2020 ◽  
Vol 236 ◽  
pp. 111263
Author(s):  
Joar Esteban Pinto Torres ◽  
Meng Yuan ◽  
Julie Goossens ◽  
Wim Versées ◽  
Guy Caljon ◽  
...  
Keyword(s):  
Pyruvate Kinase ◽  
Trypanosoma Congolense ◽  
Kinetic Characterization

scholarly journals The NTP generating activity of pyruvate kinase II is critical for apicoplast maintenance in Plasmodium falciparum

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Russell P Swift ◽  
Krithika Rajaram ◽  
Cyrianne Keutcha ◽  
Hans B Liu ◽  
Bobby Kwan ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Pyruvate Kinase ◽  
Reducing Power ◽  
Enzymatic Characterization ◽  
Parasite Survival ◽  
Nucleotide Triphosphates ◽  
Bypass Line ◽  
Stage Parasite ◽  
Blood Stage Parasite

The apicoplast of Plasmodium falciparum parasites is believed to rely on the import of three-carbon phosphate compounds for use in organelle anabolic pathways, in addition to the generation of energy and reducing power within the organelle. We generated a series of genetic deletions in an apicoplast metabolic bypass line to determine which genes involved in apicoplast carbon metabolism are required for blood-stage parasite survival and organelle maintenance. We found that pyruvate kinase II (PyrKII) is essential for organelle maintenance, but that production of pyruvate by PyrKII is not responsible for this phenomenon. Enzymatic characterization of PyrKII revealed activity against all NDPs and dNDPs tested, suggesting that it may be capable of generating a broad range of nucleotide triphosphates. Conditional mislocalization of PyrKII resulted in decreased transcript levels within the apicoplast that preceded organelle disruption, suggesting that PyrKII is required for organelle maintenance due to its role in nucleotide triphosphate generation.

scholarly journals Electrophoretic, Immunologic and Kinetic Characterization of Erythrocyte Pyruvate Kinase in the Basenji Dog with Pyruvate Kinase Deficiency

1975 ◽  
Vol 117 (2) ◽  
pp. 179-185 ◽  
Author(s):  
KOJI NAKASHIMA ◽  
SHIRO MIWA ◽  
KENJI SHINOHARA ◽  
ETSUKO ODA ◽  
MITSUAKI TAJIRI ◽  
...  
Keyword(s):  
Pyruvate Kinase ◽  
Kinetic Characterization ◽  
Pyruvate Kinase Deficiency

High-level expression in Escherichia coli, purification and kinetic characterization of Plasmodium falciparum M1-aminopeptidase

2014 ◽  
Vol 104 ◽  
pp. 103-114 ◽  
Author(s):  
Jorge González-Bacerio ◽  
Joel Osuna ◽  
Amaia Ponce ◽  
Rafael Fando ◽  
Katherine Figarella ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Plasmodium Falciparum ◽  
Kinetic Characterization ◽  
High Level Expression ◽  
Expression In Escherichia Coli ◽  
High Level

Kinetic Characterization of Anticarsia gemmatalis Digestive Serine- Proteases and the Inhibitory Effect of Synthetic Peptides

2018 ◽  
Vol 24 (11) ◽  
Author(s):  
Adriana M. Patarroyo-Vargas ◽  
Yaremis B. Merino-Cabrera ◽  
Jose C. Zanuncio ◽  
Francelina Rocha ◽  
Wellington G. Campos ◽  
...  
Keyword(s):  
Synthetic Peptides ◽  
Serine Proteases ◽  
Inhibitory Effect ◽  
Anticarsia Gemmatalis ◽  
Kinetic Characterization
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