Heterologous gene expression and functional analysis of a type III polyketide synthase from Aspergillus niger NRRL 328

2016 ◽  
Vol 473 (4) ◽  
pp. 1106-1110 ◽  
Author(s):  
Kohtaro Kirimura ◽  
Shotaro Watanabe ◽  
Keiichi Kobayashi
Keyword(s):  
Gene Expression ◽  
Functional Analysis ◽  
Aspergillus Niger ◽  
Polyketide Synthase ◽  
Heterologous Gene Expression ◽  
Heterologous Gene ◽  
Type Iii Polyketide Synthase ◽  
Type Iii

Isocoumarin formation by heterologous gene expression and modification by host enzymes

2020 ◽  
Vol 18 (26) ◽  
pp. 4946-4948 ◽  
Author(s):  
Pan Xiang ◽  
Lena Ludwig-Radtke ◽  
Wen-Bing Yin ◽  
Shu-Ming Li
Keyword(s):  
Gene Expression ◽  
Polyketide Synthase ◽  
Heterologous Gene Expression ◽  
Heterologous Gene ◽  
Endogenous Enzymes

The polyketide synthase product was converted to its methylated and hydroxylated derivatives by host endogenous enzymes.

Heterologous gene expression in Aspergillus niger: a glucoamylase-porcine pancreatic prophospholipase A2 fusion protein is secreted and processed to yield mature enzyme

Gene ◽  
1992 ◽  
Vol 122 (1) ◽  
pp. 155-161 ◽  
Author(s):  
Ian N. Roberts ◽  
David J. Jeenes ◽  
Donald A. MacKenzie ◽  
Andrew P. Wilkinson ◽  
Ian G. Sumner ◽  
...  
Keyword(s):  
Gene Expression ◽  
Aspergillus Niger ◽  
Fusion Protein ◽  
Heterologous Gene Expression ◽  
Heterologous Gene ◽  
Mature Enzyme

Cloning and characterization of a type III polyketide synthase from Aspergillus niger

2011 ◽  
Vol 21 (20) ◽  
pp. 6085-6089 ◽  
Author(s):  
Jinglin Li ◽  
Yunzi Luo ◽  
Jung-Kul Lee ◽  
Huimin Zhao
Keyword(s):  
Aspergillus Niger ◽  
Polyketide Synthase ◽  
Type Iii Polyketide Synthase ◽  
Type Iii

scholarly journals Optimization of Heterologous Gene Expression for In Vitro Evolution

BioTechniques ◽  
2001 ◽  
Vol 30 (3) ◽  
pp. 474-476 ◽  
Author(s):  
Ichiro Matsumura ◽  
Mark J. Olsen ◽  
Andrew D. Ellington
Keyword(s):  
Gene Expression ◽  
Heterologous Gene Expression ◽  
Heterologous Gene ◽  
In Vitro Evolution

Production of foreign proteins in the yeast Pichia pastoris

1995 ◽  
Vol 73 (S1) ◽  
pp. 891-897 ◽  
Author(s):  
James M. Cregg ◽  
David R. Higgins
Keyword(s):  
Gene Expression ◽  
Pichia Pastoris ◽  
Heterologous Gene Expression ◽  
Expression System ◽  
Methylotrophic Yeast ◽  
Culture Broth ◽  
Heterologous Gene ◽  
Foreign Gene ◽  
Heterologous Proteins ◽  
Yeast Pichia Pastoris

The methanol-utilizing yeast Pichia pastoris has been developed as a host system for the production of heterologous proteins of commercial interest. An industrial yeast selected for efficient growth on methanol for biomass generation, P. pastoris is readily grown on defined medium in continuous culture at high volume and density. A unique feature of the expression system is the promoter employed to drive heterologous gene expression, which is derived from the methanol-regulated alcohol oxidase I gene (AOX1) of P. pastoris, one of the most efficient and tightly regulated promoters known. The strength of the AOX1 promoter results in high expression levels in strains harboring only a single integrated copy of a foreign-gene expression cassette. Levels may often be further enhanced through the integration of multiple cassette copies into the P. pastoris genome and strategies to construct and select multicopy cassette strains have been devised. The system is particularly attractive for the secretion of foreign-gene products. Because P. pastoris endogenous protein secretion levels are low, foreign secreted proteins often appear to be virtually the only proteins in the culture broth, a major advantage in processing and purification. Key words: heterologous gene expression, methylotrophic yeast, Pichia pastoris, secretion, glycosylation.

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