Construction of an inducible stable cell line for efficient incorporation of unnatural amino acids in mammalian cells

2017 ◽  
Vol 489 (4) ◽  
pp. 490-496 ◽  
Author(s):  
Ziwei Zhang ◽  
Huan Xu ◽  
Longlong Si ◽  
Yi Chen ◽  
Bo Zhang ◽  
...  
Keyword(s):  
Amino Acids ◽  
Cell Line ◽  
Unnatural Amino Acids ◽  
Mammalian Cells ◽  
Stable Cell Line

Genetic Incorporation of Multiple Unnatural Amino Acids into Proteins in Mammalian Cells

2013 ◽  
Vol 125 (52) ◽  
pp. 14330-14333 ◽  
Author(s):  
Han Xiao ◽  
Abhishek Chatterjee ◽  
Sei-hyun Choi ◽  
Krishna M. Bajjuri ◽  
Subhash C. Sinha ◽  
...  
Keyword(s):  
Amino Acids ◽  
Unnatural Amino Acids ◽  
Mammalian Cells ◽  
Genetic Incorporation

scholarly journals Site-Specific Incorporation of Unnatural Amino Acids into Receptors Expressed in Mammalian Cells

2003 ◽  
Vol 10 (6) ◽  
pp. 573-580 ◽  
Author(s):  
Sarah L Monahan ◽  
Henry A Lester ◽  
Dennis A Dougherty
Keyword(s):  
Amino Acids ◽  
Unnatural Amino Acids ◽  
Mammalian Cells ◽  
Site Specific

scholarly journals High-Yield, Zero-Leakage Expression System with a Translational Switch Using Site-Specific Unnatural Amino Acid Incorporation

2013 ◽  
Vol 80 (5) ◽  
pp. 1718-1725 ◽  
Author(s):  
Masaomi Minaba ◽  
Yusuke Kato
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Unnatural Amino Acids ◽  
Mammalian Cells ◽  
Expression System ◽  
Trna Synthetase ◽  
Unnatural Amino Acid ◽  
Translational Efficiency ◽  
Site Specific ◽  
Content Type

ABSTRACTSynthetic biologists construct complex biological circuits by combinations of various genetic parts. Many genetic parts that are orthogonal to one another and are independent of existing cellular processes would be ideal for use in synthetic biology. However, our toolbox is still limited with respect to the bacteriumEscherichia coli, which is important for both research and industrial use. The site-specific incorporation of unnatural amino acids is a technique that incorporates unnatural amino acids into proteins using a modified exogenous aminoacyl-tRNA synthetase/tRNA pair that is orthogonal to any native pairs in a host and is independent from other cellular functions. Focusing on the orthogonality and independency that are suitable for the genetic parts, we designed novel AND gate and translational switches using the unnatural amino acid 3-iodo-l-tyrosine incorporation system inE. coli. A translational switch was turned on after addition of 3-iodo-l-tyrosine in the culture medium within minutes and allowed tuning of switchability and translational efficiency. As an application, we also constructed a gene expression system that produced large amounts of proteins under induction conditions and exhibited zero-leakage expression under repression conditions. Similar translational switches are expected to be applicable also for eukaryotes such as yeasts, nematodes, insects, mammalian cells, and plants.

scholarly journals Efficient Incorporation of Unnatural Amino Acids in Yeast, Mammalian Cells, and Stem Cells

2008 ◽  
Vol 22 (S1) ◽  
Author(s):  
Lei Wang ◽  
Wenyuan Wang ◽  
Jeffrey K Takimoto ◽  
Qiang Wang. Jack H
Keyword(s):  
Amino Acids ◽  
Stem Cells ◽  
Unnatural Amino Acids ◽  
Mammalian Cells

Genetic incorporation of unnatural amino acids into proteins in mammalian cells

2007 ◽  
Vol 4 (3) ◽  
pp. 239-244 ◽  
Author(s):  
Wenshe Liu ◽  
Ansgar Brock ◽  
Shuo Chen ◽  
Shuibing Chen ◽  
Peter G Schultz
Keyword(s):  
Amino Acids ◽  
Unnatural Amino Acids ◽  
Mammalian Cells ◽  
Genetic Incorporation

Genetic Incorporation of Multiple Unnatural Amino Acids into Proteins in Mammalian Cells

2013 ◽  
Vol 52 (52) ◽  
pp. 14080-14083 ◽  
Author(s):  
Han Xiao ◽  
Abhishek Chatterjee ◽  
Sei-hyun Choi ◽  
Krishna M. Bajjuri ◽  
Subhash C. Sinha ◽  
...  
Keyword(s):  
Amino Acids ◽  
Unnatural Amino Acids ◽  
Mammalian Cells ◽  
Genetic Incorporation

A Facile System for Encoding Unnatural Amino Acids in Mammalian Cells

2009 ◽  
Vol 121 (22) ◽  
pp. 4112-4115 ◽  
Author(s):  
Peng R. Chen ◽  
Dan Groff ◽  
Jiantao Guo ◽  
Weijia Ou ◽  
Susan Cellitti ◽  
...  
Keyword(s):  
Amino Acids ◽  
Unnatural Amino Acids ◽  
Mammalian Cells
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