Muscarinic acetylcholine receptors regulate the dephosphorylation of eukaryotic translation elongation factor 2 in SNU-407 colon cancer cells

2019 ◽  
Vol 516 (2) ◽  
pp. 424-429 ◽  
Author(s):  
Bala Murali Krishna Vasamsetti ◽  
Ziyu Liu ◽  
Yang-Seo Park ◽  
Nam Jeong Cho
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Acetylcholine Receptors ◽  
Elongation Factor ◽  
Muscarinic Acetylcholine Receptors ◽  
Colon Cancer Cells ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Eukaryotic Translation ◽  
Elongation Factor 2

scholarly journals Silencing of EEF2K (eukaryotic elongation factor-2 kinase) reveals AMPK-ULK1-dependent autophagy in colon cancer cells

Autophagy ◽  
2014 ◽  
Vol 10 (9) ◽  
pp. 1495-1508 ◽  
Author(s):  
Chuan-Ming Xie ◽  
Xiao-Yu Liu ◽  
Kathy WY Sham ◽  
Josie MY Lai ◽  
Christopher HK Cheng
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Elongation Factor ◽  
Colon Cancer Cells ◽  
Elongation Factor 2 ◽  
Eukaryotic Elongation Factor 2 ◽  
Eukaryotic Elongation Factor

scholarly journals Proteome of actin stress fibers

2021 ◽  
Author(s):  
Shiyou Liu ◽  
Tsubasa S. Matsui ◽  
Na Kang ◽  
Shinji Deguchi
Keyword(s):  
Molecular Basis ◽  
Replicative Senescence ◽  
Human Fibroblasts ◽  
Elongation Factor ◽  
Stress Fibers ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Eukaryotic Translation ◽  
Elongation Factor 2 ◽  
Protein Components

Stress fibers (SFs), which are actomyosin structures, reorganize in response to various cues to maintain cellular homeostasis. Currently, the protein components of SFs are only partially identified, limiting our understanding of their responses. Here we isolate SFs from human fibroblasts HFF-1 to determine with proteomic analysis the whole protein components and how they change with replicative senescence (RS), a state where cells decline in ability to replicate after repeated divisions. We found that at least 263 proteins are associated with SFs, and 101 of them are upregulated with RS, by which SFs become larger in size. Among them, we focused on eEF2 (eukaryotic translation elongation factor 2) as it exhibited upon RS the most significant increase in abundance. We show that eEF2 is critical to the reorganization and stabilization of SFs in senescent fibroblasts. Our findings provide a novel molecular basis for SFs to be reinforced to resist cellular senescence.

Sign in / Sign up

Export Citation Format

Share Document