UV protection and shelf life of the biological control agent Clonostachys rosea against Fusarium graminearum

AbstractRoutinely, fungal–fungal interactions (FFI) are studied on agar surfaces. However, this format restricts high-resolution dynamic imaging. To gain experimental access to FFI at the hyphal level in real-time, we developed a microfluidic platform, a FFI device. This device utilises microchannel geometry to enhance the visibility of hyphal growth and provides control channels to allow comparisons between localised and systemic effects. We demonstrate its function by investigating the FFI between the biological control agent (BCA) Clonostachys rosea and the plant pathogen Fusarium graminearum. Microscope image analyses confirm the inhibitory effect of the necrotrophic BCA and we show that a loss of fluorescence in parasitised hyphae of GFP-tagged F. graminearum coincides with the detection of GFP in mycelium of C. rosea. The versatility of our device to operate under both water-saturated and nutrient-rich as well as dry and nutrient-deficient conditions, coupled with its spatio-temporal output, opens new opportunities to study relationships between fungi.

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Clonostachys rosea associated with ponderosa and Coulter pine needles in Slovakia

Biologia ◽

10.1515/biolog-2017-0145 ◽

2017 ◽

Vol 72 (11) ◽

Author(s):

Helena Ivanová ◽

Ľudmila Hamarová ◽

Peter Pristaš

Keyword(s):

Biological Control ◽

Biological Control Agent ◽

Pathogenic Fungi ◽

Control Agent ◽

Pine Needles ◽

Plant Pathogenic Fungi ◽

Clonostachys Rosea

Abstractis an important biological control agent against various plant pathogenic fungi. We collected – necrotic ponderosa and Coulter pine needles and obtained

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Clonostachys rosea, a potential biological control agent forRhizoctonia solaniAG-3 causing black scurf on potato

Biocontrol Science and Technology ◽

10.1080/09583157.2018.1498063 ◽

2018 ◽

Vol 28 (9) ◽

pp. 895-900 ◽

Cited By ~ 4

Author(s):

Amy L. Salamone ◽

Babette Gundersen ◽

Debra Ann Inglis

Keyword(s):

Biological Control ◽

Biological Control Agent ◽

Control Agent ◽

Black Scurf ◽

Clonostachys Rosea

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The Mode of Action of Bacillus Species against Fusarium graminearum, Tools for Investigation, and Future Prospects

Toxins ◽

10.3390/toxins11100606 ◽

2019 ◽

Vol 11 (10) ◽

pp. 606 ◽

Cited By ~ 6

Author(s):

Khayalethu Ntushelo ◽

Lesiba Klaas Ledwaba ◽

Molemi Evelyn Rauwane ◽

Oluwafemi Ayodeji Adebo ◽

Patrick Berka Njobeh

Keyword(s):

Biological Control ◽

Fusarium Graminearum ◽

Plant Pathogens ◽

Biological Control Agent ◽

Control Agent ◽

Fungal Species ◽

Bacillus Species ◽

Future Studies ◽

Biochemical Processes ◽

Analytical Platforms

Fusarium graminearum is a pervasive plant pathogenic fungal species. Biological control agents employ various strategies to weaken their targets, as shown by Bacillus species, which adopt various mechanisms, including the production of bioactive compounds, to inhibit the growth of F. graminearum. Various efforts to uncover the antagonistic mechanisms of Bacillus against F. graminearum have been undertaken and have yielded a plethora of data available in the current literature. This perspective article attempts to provide a unified record of these interesting findings. The authors provide background knowledge on the use of Bacillus as a biocontrol agent as well as details on techniques and tools for studying the antagonistic mechanism of Bacillus against F. graminearum. Emphasizing its potential as a future biological control agent with extensive use, the authors encourage future studies on Bacillus as a useful antagonist of F. graminearum and other plant pathogens. It is also recommended to take advantage of the newly invented analytical platforms for studying biochemical processes to understand the mechanism of action of Bacillus against plant pathogens in general.

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