Thymoquinone prevents endoplasmic reticulum stress and mitochondria-induced apoptosis in a rat model of partial hepatic warm ischemia reperfusion

2017 ◽  
Vol 94 ◽  
pp. 964-973 ◽  
Author(s):  
Ahlem Bouhlel ◽  
Ismail Ben Mosbah ◽  
Najet Hadj Abdallah ◽  
Catherine Ribault ◽  
Roselyne Viel ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Rat Model ◽  
Ischemia Reperfusion ◽  
Warm Ischemia ◽  
Induced Apoptosis

Effect of Pioglitazone on endoplasmic reticulum stress regarding in situ perfusion rat model

2021 ◽  
pp. 1-15
Author(s):  
Vivien Telek ◽  
Luca Erlitz ◽  
Ibitamuno Caleb ◽  
Tibor Nagy ◽  
Mónika Vecsernyés ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Western Blot ◽  
Rat Model ◽  
Ischemia Reperfusion ◽  
Effective Dosage ◽  
Control Group ◽  
Histopathological Changes ◽  
In Situ Perfusion

BACKGROUND: Ischemia-reperfusion injury (IRI) can cause insufficient microcirculation of the transplanted organ and results in a diminished and inferior graft survival rate. OBJECTIVE: This study aimed to investigate the effect of different doses of an anti-diabetic drug, Pioglitazone (Pio), on endoplasmic reticulum stress and histopathological changes, using an in situ perfusion rat model. METHODS: Sixty male Wistar rats were used and were divided into six groups, consisting of the control group, vehicle-treated group and four Pio-treated groups (10, 20, 30 and 40 mg/kg Pio was administered). The rats were perfused through vena cava and an outflow on the abdominal aorta occurred. Following the experiment, kidneys and livers were collected. The level of the endoplasmic reticulum stress markers (XBP1 and Caspase 12) was analyzed using Western blot and histopathological changes were evaluated. RESULTS: Histopathological findings were correlated with the Western blot results and depict a protective effect corresponding to the elevated dosage of Pioglitazone regarding in situ perfusion rat model. CONCLUSIONS: In our study, Pioglitazone can reduce the endoplasmic reticulum stress, and the most effective dosage proved to be the 40 mg/kg Pio referencing the kidney and liver samples.

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