Repression of mineral phosphate solubilizing phenotype in the presence of weak organic acids in plant growth promoting fluorescent pseudomonads

Medicinal plants provide an enormous bioresource of potential use in modern medicine and agriculture. Phosphorous deficiency is a major constraint to plant production. Sustainable agriculture could be promoted by harnessing the plant beneficial bacteria particularly the fluorescent pseudomonads associated with the rhizosphere of plants, to mobilize soil inorganic phosphate and also to increase its bioavailability to the plants. Total five hundred seven fluorescent Pseudomonas isolates were obtained from four different Aloe barbadensis (Miller) growing locations of Varanasi. These Pseudomonas strains were further evaluated in vitro for their ability to solubilize phosphate and to produce indole acetic acid (IAA), hydrogen cyanide (HCN), siderophore and aminocyclopropane (ACC) deaminase. Total 119 fluorescent Pseudomonas isolates from the rhizospheric soil (RS) and 25 isolates from the endorhizosperic (ER) region solubilized phosphate.Whereas 53 (36.8%) Pseudomonas isolates produced IAA and siderophore, 36(25%) and 31 (21.5%) isolates, however, produced HCN and ACC deaminase. Out of 119 phosphate solubilizing bacteria (PSB) from RS region, 51 (42.9%) isolates and 9 (36%) isolates out of 25 PSBs from ER region lacked plant growth promoting traits (PGPTs). Among the phosphate solubilizing fluorescent pseudomonads showing PGPT, 59 isolates have multiple traits and showed more than two types of PGPT. A positive correlation exists between siderophore and ACC deaminase producers. Clustering by principal component analysis (PCA) showed that RS was the most important factor influencing the ecological distribution and physiological characterization of PGPT- possessing PSB. Geographical Information System (GIS) and Kriging Interpolation method was used to map and establish spatial variation of soil properties of the study site.

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Multifarious native plant growth promoting fluorescent pseudomonads associated with rhizosphere of Aloe barbadensis miller

10.7287/peerj.preprints.1903 ◽

2016 ◽

Author(s):

Anuradha Rai ◽

Pradeep K Rai ◽

Jay S Singh ◽

Surendra Singh

Keyword(s):

Plant Growth ◽

Acc Deaminase ◽

Fluorescent Pseudomonads ◽

Plant Production ◽

Geographical Information ◽

Phosphate Solubilizing Bacteria ◽

Aloe Barbadensis ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Phosphate Solubilizing

Medicinal plants provide an enormous bioresource of potential use in modern medicine and agriculture. Phosphorous deficiency is a major constraint to plant production. Sustainable agriculture could be promoted by harnessing the plant beneficial bacteria particularly the fluorescent pseudomonads associated with the rhizosphere of plants, to mobilize soil inorganic phosphate and also to increase its bioavailability to the plants. Total five hundred seven fluorescent Pseudomonas isolates were obtained from four different Aloe barbadensis (Miller) growing locations of Varanasi. These Pseudomonas strains were further evaluated in vitro for their ability to solubilize phosphate and to produce indole acetic acid (IAA), hydrogen cyanide (HCN), siderophore and aminocyclopropane (ACC) deaminase. Total 119 fluorescent Pseudomonas isolates from the rhizospheric soil (RS) and 25 isolates from the endorhizosperic (ER) region solubilized phosphate.Whereas 53 (36.8%) Pseudomonas isolates produced IAA and siderophore, 36(25%) and 31 (21.5%) isolates, however, produced HCN and ACC deaminase. Out of 119 phosphate solubilizing bacteria (PSB) from RS region, 51 (42.9%) isolates and 9 (36%) isolates out of 25 PSBs from ER region lacked plant growth promoting traits (PGPTs). Among the phosphate solubilizing fluorescent pseudomonads showing PGPT, 59 isolates have multiple traits and showed more than two types of PGPT. A positive correlation exists between siderophore and ACC deaminase producers. Clustering by principal component analysis (PCA) showed that RS was the most important factor influencing the ecological distribution and physiological characterization of PGPT- possessing PSB. Geographical Information System (GIS) and Kriging Interpolation method was used to map and establish spatial variation of soil properties of the study site.

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ISOLATION AND CHARACTERIZATION OF PLANT GROWTH PROMOTING RHIZOBACTERIA (FLUORESCENT PSEUDOMONADS) FROM ERBIL SOIL, KURDISTAN REGION-IRAQ AND USE TO IMPROVE GROWTH AND YIELD OF ZEA MAYS

Polytechnic Journal ◽

10.25156/ptj.2018.8.3.201 ◽

2018 ◽

Vol 8 (3) ◽

pp. 26-44 ◽

Cited By ~ 1

Author(s):

Aras Khudhur

Keyword(s):

Zea Mays ◽

Plant Growth ◽

Plant Growth Promoting Rhizobacteria ◽

Fluorescent Pseudomonads ◽

Growth And Yield ◽

Kurdistan Region ◽

Isolation And Characterization ◽

Plant Growth Promoting ◽

Growth Promoting

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Profiling of Plant Growth-Promoting Metabolites by Phosphate-Solubilizing Bacteria in Maize Rhizosphere

Plants ◽

10.3390/plants10061071 ◽

2021 ◽

Vol 10 (6) ◽

pp. 1071

Author(s):

Minchong Shen ◽

Jiangang Li ◽

Yuanhua Dong ◽

Hong Liu ◽

Junwei Peng ◽

...

Keyword(s):

Plant Growth ◽

Microbial Control ◽

Agricultural Practices ◽

Phosphate Solubilizing Bacteria ◽

Mean Values ◽

Plant Growth Promoting ◽

Promote Plant Growth ◽

Growth Promoting ◽

Phosphate Solubilizing ◽

Microbial Treatments

Microbial treatment has recently been attracting attention as a sustainable agricultural strategy addressing the current problems caused by unreasonable agricultural practices. However, the mechanism through which microbial inoculants promote plant growth is not well understood. In this study, two phosphate-solubilizing bacteria (PSB) were screened, and their growth-promoting abilities were explored. At day 7 (D7), the lengths of the root and sprout with three microbial treatments, M16, M44, and the combination of M16 and M44 (Com), were significantly greater than those with the non-microbial control, with mean values of 9.08 and 4.73, 7.15 and 4.83, and 13.98 and 5.68 cm, respectively. At day 14 (D14), M16, M44, and Com significantly increased not only the length of the root and sprout but also the underground and aboveground biomass. Differential metabolites were identified, and various amino acids, amino acid derivatives, and other plant growth-regulating molecules were significantly enhanced by the three microbial treatments. The profiling of key metabolites associated with plant growth in different microbial treatments showed consistent results with their performances in the germination experiment, which revealed the metabolic mechanism of plant growth-promoting processes mediated by screened PSB. This study provides a theoretical basis for the application of PSB in sustainable agriculture.

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