Enzymatic synthesis of S-phenyl-l-cysteine from keratin hydrolysis industries wastewater with tryptophan synthase

2013 ◽  
Vol 133 ◽  
pp. 635-637 ◽  
Author(s):  
Lisheng Xu ◽  
Zhiyuan Wang ◽  
Pingting Mao ◽  
Junzhong Liu ◽  
Hongjuan Zhang ◽  
...  
Keyword(s):  
Enzymatic Synthesis ◽  
Tryptophan Synthase ◽  
Keratin Hydrolysis

Enzymatic Synthesis of L-Tryptophan Catalyzed by Tryptophan Synthase in a Water/Organic Solvent Biphase System

2014 ◽  
Vol 32 (8) ◽  
pp. 1405-1410
Author(s):  
Lisheng XU ◽  
Junzhong LIU ◽  
Zhiyuan WANG ◽  
Hongjuan ZHANG ◽  
Wei LIU ◽  
...  
Keyword(s):  
Organic Solvent ◽  
Enzymatic Synthesis ◽  
Tryptophan Synthase

scholarly journals Enzymatic synthesis of Se-substituted L-selenocysteine with tryptophan synthase

FEBS Letters ◽  
1983 ◽  
Vol 161 (2) ◽  
pp. 207-209 ◽  
Author(s):  
Nobuyoshi Esaki ◽  
Hidehiko Tanaka ◽  
Edith W. Miles ◽  
Kenji Soda
Keyword(s):  
Enzymatic Synthesis ◽  
Tryptophan Synthase

scholarly journals Directed evolution  improves the enzymatic synthesis of L-5-hydroxytryptophan by an  engineered tryptophan synthase

2021 ◽  
Author(s):  
Xu Lisheng ◽  
Tingting Li ◽  
Ziyue Huo ◽  
Qiong Chen ◽  
Qiuxia Xia ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Directed Evolution ◽  
Enzymatic Synthesis ◽  
High Throughput Screening ◽  
Screening Method ◽  
Mutant Enzyme ◽  
Tryptophan Synthase ◽  
Effective Strategy ◽  
Reaction Conditions ◽  
Important Amino Acid

Abstract L-5-Hydroxytryptophan is an important amino acid that is widely used in food and medicine. In this study, L-5-hydroxytryptophan was synthesized by a modified tryptophan synthase. A direct evolution strategy was applied to engineer tryptophan synthase from Escherichia coli to improve the efficiency of L-5-hydroxytryptophan synthesis. Tryptophan synthase was modified by error-prone PCR. A high activity mutant enzyme (V231A/K382G) was obtained by a high-throughput screening method. The activity of mutant enzyme (V231A/K382G) is 3.79 times higher than that of its parent, and kcat/Km of the mutant enzyme (V231A/K382G) was 4.36 mM− 1∙s− 1. The mutant enzyme (V231A/K382G) reaction conditions for the production of L-5-hydroxytryptophan were 100 mmol/L L-serine at pH 8.5 and 35°C for 15 h, reaching a yield of L-5-hydroxytryptophan of 86.7%. Directed evolution is an effective strategy to increase the activity of tryptophan synthase.

scholarly journals Enzymatic Synthesis ofs-Substitutedl-Cysteines with Tryptophan Synthase ofEscherichia coli

1983 ◽  
Vol 47 (12) ◽  
pp. 2861-2864 ◽  
Author(s):  
Nobuyoshi Esaki ◽  
Hidehiko Tanaka ◽  
Edith Wilson Miles ◽  
Kenji Soda
Keyword(s):  
Enzymatic Synthesis ◽  
Ofescherichia Coli ◽  
Tryptophan Synthase

Effects of Polyhydroxy Compounds on Enzymatic Synthesis of L-Tryptophan Catalyzed by Tryptophan Synthase

2011 ◽  
Vol 142 (2) ◽  
pp. 282-286 ◽  
Author(s):  
Lisheng Xu ◽  
Zhiyuan Wang ◽  
Junzhong Liu ◽  
Pingting Mao ◽  
Hongjuan Zhang ◽  
...  
Keyword(s):  
Enzymatic Synthesis ◽  
Tryptophan Synthase ◽  
Polyhydroxy Compounds

scholarly journals Enzymatic synthesis of S-substituted L-cysteines with tryptophan synthase of Escherichia coli.

1983 ◽  
Vol 47 (12) ◽  
pp. 2861-2864 ◽  
Author(s):  
Nobuyoshi ESAKI ◽  
Hidehiko TANAKA ◽  
Edith Wilson MILES ◽  
Kenji SODA
Keyword(s):  
Escherichia Coli ◽  
Enzymatic Synthesis ◽  
Tryptophan Synthase

Enzymatic synthesis of l-tryptophan from hair acid hydrolysis industries wastewater with tryptophan synthase

2011 ◽  
Vol 102 (3) ◽  
pp. 3554-3557 ◽  
Author(s):  
Genhai Zhao ◽  
Junzhong Liu ◽  
Kai Dong ◽  
Fei Zhang ◽  
Hongjuan Zhang ◽  
...  
Keyword(s):  
Acid Hydrolysis ◽  
Enzymatic Synthesis ◽  
Tryptophan Synthase
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