Dilute sulfuric acid pretreatment of corn stover for enzymatic hydrolysis and efficient ethanol production by recombinant Escherichia coli FBR5 without detoxification

2013 ◽  
Vol 142 ◽  
pp. 312-319 ◽  
Author(s):  
Ayse Avci ◽  
Badal C. Saha ◽  
Gregory J. Kennedy ◽  
Michael A. Cotta
2012 ◽  
Vol 535-537 ◽  
pp. 2462-2468 ◽  
Author(s):  
Shang Xing Chen ◽  
Qiang Yong ◽  
Yong Xu ◽  
Shi Yuan Yu

The pretreatment of corn stover with dilute sulfuric acid has been investigated by varying the acid concentration (0.5%-1.25%(w/w)) and the temperature (130-160°C). The pretreatment is aimed at improving enzymatic hydrolysis and increasing the fermentability of the biomass. Given the overall sugar yield, the most favourable pretreatment condition was performed with 0.75% sulfuric acid at 150°C for 30min and then with an enzyme loading of cellulase 15 FPU per gram of cellulose, and it resulted in a total of 49.74g glucose and xylose from 100g dry corn stover. The fiber physical feature, structure and property of pretreated residues were studied with Scanning Electron Microscope (SEM) and Fourier transform infrared spectroscopy. The SEM pictures indicated that the biomass structure was deformed and its fibers were exposed by the pretreatment. FTIR study showed that lignin and hemicellulose were partially removed during the diluted sulfuric acid pretreatment.


2008 ◽  
Vol 42 (1) ◽  
pp. 84-91 ◽  
Author(s):  
Ignacio Ballesteros ◽  
Mercedes Ballesteros ◽  
Paloma Manzanares ◽  
M. José Negro ◽  
J. Miguel Oliva ◽  
...  

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