scholarly journals Cloning and expression of an endoglucanase gene from the thermotolerant fungus Aspergillus fumigatus DBiNU-1 in Kluyveromyces lactis

2018 ◽  
Vol 49 (3) ◽  
pp. 647-655 ◽  
Author(s):  
Budsayachat Rungrattanakasin ◽  
Siripong Premjet ◽  
Sudarat Thanonkeo ◽  
Preekamol Klanrit ◽  
Pornthap Thanonkeo
Keyword(s):  
Aspergillus Fumigatus ◽  
Kluyveromyces Lactis ◽  
Cloning And Expression ◽  
Endoglucanase Gene ◽  
Thermotolerant Fungus

Sequential cold-sensitive mutations in Aspergillus fumigatus

1978 ◽  
Vol 24 (2) ◽  
pp. 84-88 ◽  
Author(s):  
Ole Nielsen ◽  
Kenneth F. Gregory
Keyword(s):  
Aspergillus Fumigatus ◽  
Type A ◽  
Maximum Growth ◽  
Cold Sensitivity ◽  
Nonpermissive Temperature ◽  
Wild Type ◽  
Cold Sensitive ◽  
Wild Type Parent ◽  
Thermotolerant Fungus

Mutants of the thermotolerant fungus Aspergillus fumigatus I-21 (ATCC 32722) unable to grow at 37 °C were sought. Cold-sensitive mutants were enriched from progeny spores of γ-irradiated conidia by two or more incubations at various nonpermissive temperatures alternating with filtrations through cheesecloth. The approximate minimum, optimum, and maximum growth temperatures of the parent were 12, 40, and 50 °C, respectively. Mutants unable to grow at 37 °C were not successfully isolated directly from the wild type. A mutant unable to grow at 25 °C was isolated and mutations further increasing the cold sensitivity by increments of 3–5 °C were found to occur. Mutants completely unable to grow at 37 °C were obtained by five sequential mutations. All mutants grew as fast as the wild-type parent at 45 °C and higher. Each mutant produced revenants able to grow not only at the nonpermissive temperature used for its isolation but also at lower temperatures.

Cloning and expression of GH11 xylanase gene from Aspergillus fumigatus MKU1 in Pichia pastoris

2009 ◽  
Vol 108 (1) ◽  
pp. 24-29 ◽  
Author(s):  
Marimuthu Jeya ◽  
Sairam Thiagarajan ◽  
Jung-Kul Lee ◽  
Paramasamy Gunasekaran
Keyword(s):  
Pichia Pastoris ◽  
Aspergillus Fumigatus ◽  
Cloning And Expression ◽  
Xylanase Gene

scholarly journals Cloning and expression of β-1, 4-endoglucanase gene from Bacillus subtilis isolated from soil long term irrigated with effluents of paper and pulp mill

2014 ◽  
Vol 169 (9-10) ◽  
pp. 693-698 ◽  
Author(s):  
Sangeeta Pandey ◽  
Jyoti Kushwah ◽  
Rameshwar Tiwari ◽  
Ram Kumar ◽  
Vishal Singh Somvanshi ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Pulp Mill ◽  
Cloning And Expression ◽  
Endoglucanase Gene ◽  
Paper And Pulp

Cloning and Expression of Endoglucanase Gene from Thermophilic Bacteria Bacillus sp. RP1

2014 ◽  
Vol 8 (4) ◽  
pp. 170-176 ◽  
Author(s):  
MAELITA RAMDANI MOEIS ◽  
◽  
DESSY NATALIA ◽  
RAHMA WIDYA NINGRUM ◽  
ARI DWIJAYANTI ◽  
...  
Keyword(s):  
Thermophilic Bacteria ◽  
Cloning And Expression ◽  
Bacillus Sp ◽  
Endoglucanase Gene
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