Establishment of cementoblast cell lines from rat cementum lining cells by transfection with temperature-sensitive simian virus-40 T-antigen gene

Bone ◽  
2005 ◽  
Vol 37 (2) ◽  
pp. 220-226 ◽  
Author(s):  
Masae Kitagawa ◽  
Shoji Kitagawa ◽  
Yasusei Kudo ◽  
Ikuko Ogawa ◽  
Mutsumi Miyauchi ◽  
...  
Keyword(s):  
Cell Lines ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Temperature Sensitive ◽  
Antigen Gene

Establishment of Gastric Surface Mucous Cell Lines from Transgenic Mice Harboring Temperature-Sensitive Simian Virus 40 Large T-Antigen Gene

1993 ◽  
Vol 209 (2) ◽  
pp. 382-387 ◽  
Author(s):  
Norifumi Sugiyama ◽  
Yoshiaki Tabuchi ◽  
Tadashi Horiuchi ◽  
Masuo Obinata ◽  
Mitsuru Furusawa
Keyword(s):  
Transgenic Mice ◽  
Cell Lines ◽  
Mucous Cell ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Temperature Sensitive ◽  
Large T Antigen ◽  
Antigen Gene ◽  
Surface Mucous Cell

scholarly journals Cell lines established by a temperature-sensitive simian virus 40 large-T-antigen gene are growth restricted at the nonpermissive temperature.

1989 ◽  
Vol 9 (4) ◽  
pp. 1672-1681 ◽  
Author(s):  
P S Jat ◽  
P A Sharp
Keyword(s):  
Cell Lines ◽  
Growth Arrest ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Temperature Sensitive ◽  
Permissive Temperature ◽  
Nonpermissive Temperature ◽  
Large T Antigen ◽  
Clonal Cell Lines

The thermolabile large T antigen, encoded by the simian virus 40 early-region mutant tsA58, was used to establish clonal cell lines derived from rat embryo fibroblasts. These cell lines grew continuously at the permissive temperature but upon shift-up to the nonpermissive temperature showed rapidly arrested growth. The growth arrest occurred in either the G1 or G2 phase of the cell cycle. After growth arrest, the cells remained metabolically active as assayed by general protein synthesis and the ability to exclude trypan blue. The inability of these cell lines to divide at the nonpermissive temperature was not readily complemented by the exogenous introduction of other nuclear oncogenes. This finding suggests that either these genes establish cells via different pathways or that immortalization by one oncogene results in a finely balanced cellular state which cannot be adequately complemented by another establishment gene.

Establishment of a novel chondrocyte-like cell line derived from transgenic mice harboring the temperature-sensitive simian virus 40 large T-antigen gene

2009 ◽  
Vol 11 (11) ◽  
pp. 1646-1654 ◽  
Author(s):  
Nobuko Mataga ◽  
Masato Tamura ◽  
Nobuyuki Yanai ◽  
Tamayuki Shinomura ◽  
Koji Kimata ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Cell Line ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Temperature Sensitive ◽  
Large T Antigen ◽  
Antigen Gene
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