Evaluation of the relative mineral-binding affinities of clinically-relevant bisphosphonates by hydroxyapatite-column chromatography

Bone ◽  
2008 ◽  
Vol 43 ◽  
pp. S92
Author(s):  
Zhidao Xia ◽  
Xuchen Duan ◽  
Rachel M Locklin ◽  
Mike Quijano ◽  
Roy L.M. Dobson ◽  
...  
Keyword(s):  
Column Chromatography ◽  
Binding Affinities ◽  
Hydroxyapatite Column ◽  
Mineral Binding

Refining the use of hydroxyapatite column chromatography to reveal differences in relative binding affinities of bisphosphonates

Bone ◽  
2006 ◽  
Vol 38 (3) ◽  
pp. 55 ◽  
Author(s):  
M.A. Lawson ◽  
Z. Xia ◽  
J.T. Triffitt ◽  
F.H. Ebetino ◽  
R.J. Phipps ◽  
...  
Keyword(s):  
Column Chromatography ◽  
Binding Affinities ◽  
Relative Binding ◽  
Hydroxyapatite Column

scholarly journals Phospholipid-dependence of oestrone UDP-glucuronyltransferase and p-nitrophenol UDP-glucuronyltransferase

1979 ◽  
Vol 179 (1) ◽  
pp. 59-65 ◽  
Author(s):  
R H Tukey ◽  
R E Billings ◽  
A P Autor ◽  
T R Tephly
Keyword(s):  
Fatty Acids ◽  
Catalytic Activity ◽  
Column Chromatography ◽  
Unsaturated Fatty Acids ◽  
The Other ◽  
Glucuronyl Transferase ◽  
Hydroxyapatite Column

Hepatic UDP-glucuronyltransferase activity was resolved into two fractions, one exhibiting oestrone glucuronyltransferase activity and the other exhibiting p-nitrophenol glucuronyltransferase activity. Hydroxyapatite-column chromatography removed greater than 95% of the phospholipids from both preparations. The partially purified delipidated enzymes were essentially devoid of catalytic activity, but activities were restored by the addition of phospholipids or phosphatidylcholine mixtures containing various saturated and unsaturated fatty acids. Both oestrone and p-nitrophenol glucuronyl-transferase activities were reconstituted to similar degrees with the phosphatidylcholine mixtures. When purified phospholipids were tested, phosphatidylcholine and lysophosphatidylcholine were most effective in restoring activity, whereas phosphatidylethanolamine was the least effective. These results further suggest that oestrone and p-nitrophenol UDP-glucuronyltransferases are dependent on phospholipids for their activity.

Hydroxyapatite column chromatography of reconstitution products of TMV: Some properties of the isolated fractions

Virology ◽  
1972 ◽  
Vol 49 (2) ◽  
pp. 475-485 ◽  
Author(s):  
H Guilley ◽  
C Stussi ◽  
J.C Thouvenel ◽  
P Pfeiffer ◽  
L Hirth
Keyword(s):  
Column Chromatography ◽  
Hydroxyapatite Column
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