Remarkable increase in 14C-acetate uptake in an epilepsy model rat brain induced by lithium–pilocarpine

Fluorocitrate, a selective astrocytic toxin, was microinjected into the right striatum of rat brain, and the regional distribution of 14C-acetate was measured using autoradiography. A significant reduction (more than 80%) in 14C-acetate uptake over a 5-minute period was observed in the right striatum, compared with that in the left striatum (saline infused), 4 hours after fluorocitrate (1 nmol/μL) infusion. This effect was transient, and 14C-acetate uptake had almost returned to normal at 24 hours after the fluorocitrate infusion. In contrast, the regional blood flow in the striatum, as determined using 14C-iodoamphetamine, was significantly increased by the fluorocitrate infusion. The present observations indicate that 14C-acetate uptake might be a useful characteristic for examining astrocytic energy metabolism in the intact brain.

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Role of NMDA receptor upon [14C]acetate uptake into intact rat brain

Annals of Nuclear Medicine ◽

10.1007/s12149-008-0216-2 ◽

2009 ◽

Vol 23 (2) ◽

pp. 143-147 ◽

Cited By ~ 6

Author(s):

Shinichiro Hirose ◽

Sotaro Momosaki ◽

Rie Hosoi ◽

Kohji Abe ◽

Antony Gee ◽

...

Keyword(s):

Nmda Receptor ◽

Rat Brain ◽

Acetate Uptake

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Sensitive Reduction in 14C-acetate Uptake in a Short-term Ischemic Rat Brain

Journal of Stroke and Cerebrovascular Diseases ◽

10.1016/j.jstrokecerebrovasdis.2006.11.005 ◽

2007 ◽

Vol 16 (2) ◽

pp. 77-81 ◽

Cited By ~ 19

Author(s):

Rie Hosoi ◽

Yuto Kashiwagi ◽

Miwa Tokumura ◽

Kohji Abe ◽

Jun Hatazawa ◽

...

Keyword(s):

Rat Brain ◽

Short Term ◽

Acetate Uptake

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Effect of reactive cell density on net [2-14C]acetate uptake into rat brain: labeling of clusters containing GFAP+- and lectin+-immunoreactive cells

Neurochemistry International ◽

10.1016/s0197-0186(02)00138-9 ◽

2003 ◽

Vol 42 (5) ◽

pp. 359-374 ◽

Cited By ~ 7

Author(s):

Neslihan Cetin ◽

Kelly Ball ◽

Murat Gokden ◽

Nancy F Cruz ◽

Gerald A Dienel

Keyword(s):

Rat Brain ◽

Cell Density ◽

Reactive Cell ◽

Acetate Uptake

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Existence of DNA in yeast microbodies

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100082029 ◽

1978 ◽

Vol 36 (2) ◽

pp. 232-233

Author(s):

Masako Osumi ◽

Misuzu Nagano ◽

Hiroko Kazama

Keyword(s):

Catalase Activity ◽

Buoyant Density ◽

Model System ◽

Contour Length ◽

Native State ◽

Normal Alkane ◽

Remarkable Increase ◽

Dna Molecule ◽

Mitochondrial Dnas

We have found that microbodies appeared profusely together with a remarkable increase in catalase activity in normal alkane-grown cells of hydrocarbon-utilizing Candida yeasts, and that the microbodies multiplied by division in these cells. These features of Candida yeasts seem to provide a useful model system for studies on the biogenesis of the microbody. Subsequently, we have succeeded in isolation of Candida microbodies in an apparently native state, as judged biochemically and morphologically. The presence of DNA in the purified microbody fraction thus obtained was proved by the diphenylamine method. DNA molecule of about 15 urn in contour length was released from an isolated microbody. The physicochemical analyses of the microbody DNA revealed that its buoyant density differed from nuclear and mitochondrial DNAs. All these results lead us to the possibility that there is a novel type of DNA in microbodies.

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