Virtual constraint control of Knee-Ankle prosthesis using an improved estimate of the thigh phase-variable

2022 ◽  
Vol 73 ◽  
pp. 103366
Author(s):  
Awais Naeem ◽  
Mohsin Rizwan ◽  
Hafiz Farhan Maqbool ◽  
Muhammad Ahsan ◽  
Ali Raza ◽  
...  
IEEE Access ◽  
2019 ◽  
Vol 7 ◽  
pp. 109840-109855 ◽  
Author(s):  
Siavash Rezazadeh ◽  
David Quintero ◽  
Nikhil Divekar ◽  
Emma Reznick ◽  
Leslie Gray ◽  
...  

2021 ◽  
Author(s):  
Daphna Raz ◽  
Edgar Bolivar-Nieto ◽  
Necmiye Ozay ◽  
Robert D. Gregg

2021 ◽  
Vol 60 (1) ◽  
pp. 67-73 ◽  
Author(s):  
Calvin J. Rushing ◽  
Rona Law ◽  
Christopher F. Hyer

2000 ◽  
Vol 68 (2) ◽  
pp. 871-876 ◽  
Author(s):  
Li Liu ◽  
Kevin Dybvig ◽  
Victor S. Panangala ◽  
Vicky L. van Santen ◽  
Christopher T. French

ABSTRACT Mycoplasma gallisepticum, the cause of chronic respiratory infections in the avian host, possesses a family of M9/pMGA genes encoding an adhesin(s) associated with hemagglutination. Nucleotide sequences of M9/pMGA gene family members indicate extensive sequence similarity in the promoter regions of both the transcribed and silent genes. The mechanism that regulates M9/pMGA gene expression is unknown, but studies have revealed an apparent correlation between gene expression and the number of tandem GAA repeat motifs located upstream of the putative promoter. In this study, transposon Tn4001was used as a vector with the Escherichia coli lacZ gene as the reporter system to examine the role of the GAA repeats in M9/pMGA gene expression in M. gallisepticum. A 336-bp M9 gene fragment (containing the GAA repeat region, the promoter, and the translation start codon) was amplified by PCR, ligated with alacZ gene from E. coli, and inserted into the Tn4001-containing plasmid pISM2062. This construct was transformed into M. gallisepticum PG31. Transformants were filter cloned on agar supplemented with 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside (X-Gal) to monitor lacZ gene expression on the basis of blue/white color selection. Several cycles of filter cloning resulted in cell lineages in which lacZ gene expression alternated between the On and Off states in successive generations of progeny clones. The promoter regions of the M9-lacZ hybrid genes of individual progeny clones were amplified by PCR and sequenced. The only differences between the promoter regions of the blue and white colonies were in the number of GAA repeats. Clones that expressedlacZ had exactly 12 tandem copies of the GAA repeat. Clones that did not express lacZ invariably had either more than 12 (14 to 16) or fewer than 12 (5 to 11) GAA repeats. Southern analysis of M. gallisepticum chromosomal DNA confirmed that the phase-variable expression of the lacZ reporter gene was not caused by Tn4001 transposition. These data strongly indicate that changes in the length of the GAA repeat region are responsible for regulating M9/pMGA gene expression.


Author(s):  
Pierluigi Colli ◽  
Gianni Gilardi ◽  
Jürgen Sprekels

AbstractIn the recent paper “Well-posedness and regularity for a generalized fractional Cahn–Hilliard system” (Colli et al. in Atti Accad Naz Lincei Rend Lincei Mat Appl 30:437–478, 2019), the same authors have studied viscous and nonviscous Cahn–Hilliard systems of two operator equations in which nonlinearities of double-well type, like regular or logarithmic potentials, as well as nonsmooth potentials with indicator functions, were admitted. The operators appearing in the system equations are fractional powers $$A^{2r}$$ A 2 r and $$B^{2\sigma }$$ B 2 σ (in the spectral sense) of general linear operators A and B, which are densely defined, unbounded, selfadjoint, and monotone in the Hilbert space $$L^2(\Omega )$$ L 2 ( Ω ) , for some bounded and smooth domain $$\Omega \subset {{\mathbb {R}}}^3$$ Ω ⊂ R 3 , and have compact resolvents. Existence, uniqueness, and regularity results have been proved in the quoted paper. Here, in the case of the viscous system, we analyze the asymptotic behavior of the solution as the parameter $$\sigma $$ σ appearing in the operator $$B^{2\sigma }$$ B 2 σ decreasingly tends to zero. We prove convergence to a phase relaxation problem at the limit, and we also investigate this limiting problem, in which an additional term containing the projection of the phase variable on the kernel of B appears.


1998 ◽  
Vol 22 ◽  
pp. S831-S834 ◽  
Author(s):  
S.H. Yang ◽  
P.W.H. Chung ◽  
X.Z. Wang ◽  
C. McGreavy

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