Arsenic toxicity and its mitigation in ectomycorrhizal fungus Hebeloma cylindrosporum through glutathione biosynthesis

Chemosphere ◽  
2020 ◽  
Vol 240 ◽  
pp. 124914 ◽  
Author(s):  
Shikha Khullar ◽  
M. Sudhakara Reddy
Keyword(s):  
Ectomycorrhizal Fungus ◽  
Arsenic Toxicity ◽  
Hebeloma Cylindrosporum ◽  
Glutathione Biosynthesis

Isolation and characterization of nitrate reductase deficient mutants of the ectomycorrhizal fungus Hebeloma cylindrosporum

1998 ◽  
Vol 140 (2) ◽  
pp. 311-318 ◽  
Author(s):  
ROLAND MARMEISSE ◽  
PATRICIA JARGEAT ◽  
FRANCOISE WAGNER ◽  
GILLES GAY ◽  
JEAN-CLAUDE DEBAUD
Keyword(s):  
Nitrate Reductase ◽  
Ectomycorrhizal Fungus ◽  
Hebeloma Cylindrosporum ◽  
Isolation And Characterization

Regulation of genes involved in nitrogen utilization on different C/N ratios and nitrogen sources in the model ectomycorrhizal fungus Hebeloma cylindrosporum

Mycorrhiza ◽  
2012 ◽  
Vol 22 (7) ◽  
pp. 515-524 ◽  
Author(s):  
Meghan Avolio ◽  
Tobias Müller ◽  
Anja Mpangara ◽  
Michael Fitz ◽  
Ben Becker ◽  
...  
Keyword(s):  
Nitrogen Sources ◽  
Ectomycorrhizal Fungus ◽  
Nitrogen Utilization ◽  
Hebeloma Cylindrosporum

scholarly journals Different Patterns of Regulation for the Copper and Cadmium Metallothioneins of the Ectomycorrhizal Fungus Hebeloma cylindrosporum

2009 ◽  
Vol 75 (8) ◽  
pp. 2266-2274 ◽  
Author(s):  
G. Ramesh ◽  
G. K. Podila ◽  
G. Gay ◽  
R. Marmeisse ◽  
M. S. Reddy
Keyword(s):  
Single Copy ◽  
Regulatory Elements ◽  
Ectomycorrhizal Fungus ◽  
Pcr Analysis ◽  
Mrna Accumulation ◽  
Hebeloma Cylindrosporum ◽  
Expression Levels ◽  
Reverse Transcription Pcr ◽  
Mutant Strains ◽  
Survival And Growth

ABSTRACT Metallothioneins (MTs) are small cysteine-rich peptides involved in metal homeostasis and detoxification. We have characterized two MT genes, HcMT1 and HcMT2, from the ectomycorrhizal fungus Hebeloma cylindrosporum in this study. Expression of HcMT1 and HcMT2 in H. cylindrosporum under metal stress conditions was studied by competitive reverse transcription-PCR analysis. The full-length cDNAs were used to perform functional complementation in mutant strains of Saccharomyces cerevisiae. As revealed by heterologous complementation assays in yeast, HcMT1 and HcMT2 each encode a functional polypeptide capable of conferring increased tolerance against Cd and Cu, respectively. The expression levels of HcMT1 were observed to be at their maximum at 24 h, and they increased as a function of Cu concentration. HcMT2 was also induced by Cu, but the expression levels were lower than those for HcMT1. The mRNA accumulation of HcMT1 was not influenced by Cd, whereas Cd induced the transcription of HcMT2. Zn, Pb, and Ni did not affect the transcription of HcMT1 or of HcMT2. Southern blot analysis revealed that both of these genes are present as a single copy in H. cylindrosporum. While the promoters of both HcMT1 and HcMT2 contained the standard stress response elements implicated in the metal response, the numbers and varieties of potential regulatory elements were different in these promoters. These results show that ectomycorrhizal fungi encode different MTs and that each of them has a particular pattern of expression, suggesting that they play critical specific roles in improving the survival and growth of ectomycorrhizal trees in ecosystems contaminated by heavy metals.

Efficiency in a forest nursery of an ectomycorrhizal fungus inoculum produced in a fermentor and entrapped in polymeric gels

1985 ◽  
Vol 63 (9) ◽  
pp. 1664-1668 ◽  
Author(s):  
F. Le Tacon ◽  
G. Jung ◽  
J. Mugnier ◽  
P. Michelot ◽  
C. Mauperin
Keyword(s):  
Norway Spruce ◽  
Seedling Growth ◽  
Ectomycorrhizal Fungus ◽  
Douglas Fir ◽  
Forest Nursery ◽  
Thelephora Terrestris ◽  
Hebeloma Cylindrosporum ◽  
Polymeric Gels ◽  
Mycorrhizal Development ◽  
High Metabolic Activity

In a nursery in the centre of France, we have compared the effect of different forms of an ectomycorrhizal fungus inoculum (Hebeloma cylindrosporum) on growth and mycorrhizal development of Douglas-fir and Norway spruce after soil fumigation. Compared with inoculum produced on peat and vermiculite, the inoculum produced in a fermentor and entrapped in polymeric gels significantly improved Douglas-fir and Norway spruce seedling growth. The Hebeloma mycorrhizal index was not significantly different between the different forms of inocula. The superiority of the inoculum produced in the fermentor and entrapped in gels is probably related to the high metabolic activity of the mycelium and to the protection given by the polymers after the incorporation of the inoculum into the soil. Hebeloma cylindrosporum stimulates seedling growth even in the presence of Thelephora terrestris mycorrhiza.

Intraspecific genetic variation of acid phosphatase activity in monokaryotic and dikaryotic populations of the ectomycorrhizal fungus Hebeloma cylindrosporum

1991 ◽  
Vol 69 (4) ◽  
pp. 808-813 ◽  
Author(s):  
J. P. Meysselle ◽  
G. Gay ◽  
J. C. Debaud
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Specific Activity ◽  
Intraspecific Variability ◽  
Ectomycorrhizal Fungus ◽  
Hebeloma Cylindrosporum ◽  
Single Strain ◽  
Wild Strains

Intraspecific variability of acid phosphatase activity and mycelial growth of the ectomycorrhizal fungus Hebeloma cylindrosporum Romagnesi was examined because of the role of this enzyme activity in the phosphate nutrition of the fungus and consequently of mycorrhizal host plants. Interstrain variation was studied with 11 wild strains, and intrastrain variability was studied with 20 sib-monokaryons and 50 reconstituted dikaryons, progeny of the HC1 fruiting strain. The range of variation of acid phosphatase activity among wild dikaryotic mycelia was the same as that among sib-monokaryons or dikaryons belonging to the progeny of a single strain. The total phosphatase activity of the wild strains ranged from 5.70 to 96.0 total milliunits (TmU). It ranged from 11.1 to 120.5 TmU within sib-monokaryons and from 34.2 to 178.1 TmU for reconstituted dikaryons. Specific phosphatase activity of wild dikaryons ranged from 48.5 international milliunits (ImU) to 675.6 ImU, whereas the ranges of variation among sib-monokaryons and reconstituted dikaryons were, respectively, 85.3–791.0 and 270.7–816.1 ImU. On average, sib monokaryons and reconstituted dikaryons had lower activity than their parental dikaryon. However, four reconstituted dikaryons had a higher specific activity than the original dikaryon HC1. The growth of the studied mycelia also varied, but in a narrower range (from 97.1 to 151.6 μg protein per culture for wild dikaryons, from 130.1 to 199.1 μg for sib-monokaryons, and from 160.6 to 275.9 μg for reconstituted dikaryons). No correlation could be detected between specific acid phosphatase activity and growth rate in pure culture within the different monokaryotic or dikaryotic populations studied. These results demonstrate the possibility of obtaining, by intrastrain crossings, mycelia having higher phosphatase activity than the parental wild strains. The characteristics of the different mycelia are discussed in relation to a selection program and their putative spatial distribution in natural conditions. Key words: acid phosphatase, ectomycorrhizal fungus, intraspecific variation, monokaryon, dikaryon, Hebeloma cylindrosporum.

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