A hydrophilic immobilized trypsin reactor with N-vinyl-2-pyrrolidinone modified polymer microparticles as matrix for highly efficient protein digestion with low peptide residue

2012 ◽  
Vol 1246 ◽  
pp. 111-116 ◽  
Author(s):  
Hao Jiang ◽  
Huiming Yuan ◽  
Yu Liang ◽  
Simin Xia ◽  
Qun Zhao ◽  
...  
Keyword(s):  
Protein Digestion ◽  
Peptide Residue ◽  
Highly Efficient ◽  
Modified Polymer ◽  
Immobilized Trypsin ◽  
Polymer Microparticles

Facile synthesis of enzyme–inorganic hybrid nanoflowers and their application as an immobilized trypsin reactor for highly efficient protein digestion

RSC Advances ◽  
2014 ◽  
Vol 4 (27) ◽  
pp. 13888-13891 ◽  
Author(s):  
Zian Lin ◽  
Yun Xiao ◽  
Ling Wang ◽  
Yuqing Yin ◽  
Jiangnan Zheng ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Immobilized Enzyme ◽  
Protein Digestion ◽  
Enzyme Reactor ◽  
Facile Synthesis ◽  
Inorganic Hybrid ◽  
Immobilized Enzyme Reactor ◽  
Highly Efficient ◽  
Novel Approach ◽  
Immobilized Trypsin

Hybrid nanoflowers were synthesized by a novel approach. The nanoflowers exhibited an enhanced enzymatic activity and can be used as an immobilized enzyme reactor (IMER) for highly efficient protein digestion.

scholarly journals Towards automation in protein digestion: Development of a monolithic trypsin immobilized reactor for highly efficient on-line digestion and analysis

Talanta ◽  
2017 ◽  
Vol 167 ◽  
pp. 143-157 ◽  
Author(s):  
Marina Naldi ◽  
Urh Černigoj ◽  
Ales Štrancar ◽  
Manuela Bartolini
Keyword(s):  
Protein Digestion ◽  
Highly Efficient ◽  
On Line

Rational synthesis of MoS2-based immobilized trypsin for rapid and effective protein digestion

Talanta ◽  
2018 ◽  
Vol 179 ◽  
pp. 393-400 ◽  
Author(s):  
Chaoshuang Xia ◽  
Heping Wang ◽  
Fenglong Jiao ◽  
Fangyuan Gao ◽  
Qiong Wu ◽  
...  
Keyword(s):  
Protein Digestion ◽  
Immobilized Trypsin
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