Polystyrene bound silica monolith particles of reduced size as stationary phase of excellent separation efficiency in high performance liquid chromatograhy

2019 ◽  
Vol 1594 ◽  
pp. 72-81 ◽  
Author(s):  
Ashraf Ali ◽  
Genlin Sun ◽  
Jeong Soo Kim ◽  
Won Jo Cheong
Keyword(s):  
Stationary Phase ◽  
High Performance ◽  
Separation Efficiency ◽  
Silica Monolith

Chiral Separation of Racemic Naproxen by High-Performance Liquid Chromatography with β-CD/SiO2 as the Chiral Stationary Phase

2014 ◽  
Vol 97 (1) ◽  
pp. 121-127 ◽  
Author(s):  
Lili Wang ◽  
Jian Deng ◽  
Xuezhen Ji ◽  
Wanbin Liu ◽  
Jun Liang ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Chiral Separation ◽  
Photoelectron Spectroscopy ◽  
High Performance ◽  
Chiral Stationary Phase ◽  
Separation Efficiency ◽  
Phosphate Buffer Solution ◽  
Buffer Solution ◽  
Scanning Calorimetry ◽  
Column Temperature

Abstract A novel derivative β-cyclodextrin (β-CD) bonded silica gel (β-CD/SiO2) was prepared via carbodiimide activation and used as a chiral stationary phase (CSP). The β-CD/SiO2 CSP was characterizedby X-ray photoelectron spectroscopy and FTIR spectroscopy to prove the successful immobilization of β-CD onto the surface of the silica gel.The thermal behavior of the CSP was studied by differential scanning calorimetry and then used forthe chiral separation of racemic naproxen using HPLC. Several factors affecting the separation efficiency of naproxen enantiomers were investigatedsystematically. The following parameters were selected: methanol–0.01 M phosphate buffer solution (85 + 15, v/v, pH 3.5) was the mobile phase, the flow rate was 1.0 mL/min, and the column temperature was 35°C. Under optimal conditions, the racemic naproxen was efficiently separated with a resolution factor (RS) of 1.70 and a separation factor (α) of 1.25. This method was successfully used for the enantiomeric separation and purity assessment of naproxen in commercial naproxen sustained release tablets.

Magnetic Microparticles@UiO-67 Core-Shell Composites as a Novel Stationary Phase for High Performance Liquid Chromatography

2014 ◽  
Vol 703 ◽  
pp. 73-76 ◽  
Author(s):  
Wei Wei Qin ◽  
Martin E. Silvestre ◽  
Matthias Franzreb
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Stationary Phase ◽  
High Performance ◽  
Separation Efficiency ◽  
Core Shell ◽  
Moderate Pressure ◽  
Fast Kinetics ◽  
Phenol Derivatives ◽  
Magnetic Silica

Metal-organic framework UiO-67 was explored as a novel stationary phase for high performance liquid chromatography (HPLC). UiO-67 was, for the first time, homogeneously coated on carboxyl functionalized magnetic silica microparticles at low temperature (45 °C) by using a recently introduced liquid phase epitaxy (LPE) process. HPLC runs using the synthesized core-shell microparticles as stationary phase showed baseline separation for three phenol derivatives, applying gradient elution using acetonitrile and water as mobile phase. It also showed that UiO-67 has the largest affinity for 2,6-dichlorophenol among the phenol derivatives tested. The comparison of core-shell microparticles with 20 and 55 layers, respectively, of UiO-67 grown on the magnetic silica core proof that the UiO-67 shell determines separation behavior. Therefore, the use of UiO-67 core-shell microparticles as a stationary phase combines the advantages of a thin, homogenous MOF shell showing fast kinetics and good separation efficiency with the advantages of spherical silica microparticle cores offering high mechanical robustness and moderate pressure drop.

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