Comparison of flagellin and an oil-emulsion adjuvant in inactivated Newcastle disease vaccine in stimulation of immunogenic parameters

In order to evaluate the efficacy of inactivated oil-emulsion Newcastle disease vaccine (commercially available on market in Serbia) we carried out the potency test in chickens, using hemagglutination inhibition (HI) test for detection of antibodies. Th e values of HI titers in the serum before and three weeks aft er vaccination revealed immunoconversion (IC) in chickens, and indicated potential diff erences in the immunoconversion values between two groups vaccinated with monovalent and polyvalent vaccine. In the period 2010-2012, 27 vaccines were tested, there of 21 polyvalent and six monovalent ones. Th ree weeks aft er the vaccination, HI titers of all vaccines were high. Th e average IC values were calculated as log2 (HI titer). The values ranged from 2.2 to 8.2 for polyvalent vaccine, whereas average values for monovalent vaccines were in the range from 3.2 to 6.3. Three weeks post vaccination; no statistically signifi cant diff erences were recorded in the immune response between the tested groups of birds (vaccinated with monovalent and polyvalent vaccines). This supports the fact that both tested vaccines demonstrated good potency to creating immunity against ND in vaccinated birds.

Download Full-text

Vaccination of Broiler Chicks from Breeder Flocks Immunized with a Live or Inactivated Oil Emulsion Newcastle Disease Vaccine ,

Poultry Science ◽

10.3382/ps.0611621 ◽

1982 ◽

Vol 61 (8) ◽

pp. 1621-1629 ◽

Cited By ~ 11

Author(s):

C.S. EIDSON ◽

S.G. THAYER ◽

P. VILLEGAS ◽

S.H. KLEVEN

Keyword(s):

Newcastle Disease ◽

Broiler Chicks ◽

Oil Emulsion ◽

Newcastle Disease Vaccine

Download Full-text

Field Trials with an Oil Emulsion Newcastle Disease Vaccine in Broiler Breeders ,

Poultry Science ◽

10.3382/ps.0590702 ◽

1980 ◽

Vol 59 (4) ◽

pp. 702-707 ◽

Cited By ~ 7

Author(s):

C.S. EIDSON ◽

P. VILLEGAS ◽

S.H. KLEVEN

Keyword(s):

Newcastle Disease ◽

Field Trials ◽

Broiler Breeders ◽

Oil Emulsion ◽

Newcastle Disease Vaccine

Download Full-text

CAvant® WO-60 as an Effective Immunological Adjuvant for Avian Influenza and Newcastle Disease Vaccine

Frontiers in Veterinary Science ◽

10.3389/fvets.2021.730700 ◽

2021 ◽

Vol 8 ◽

Author(s):

Eun-Seo Lee ◽

Young-Jung Shim ◽

W. A. Gayan Chathuranga ◽

Young-Hoon Ahn ◽

In-Joong Yoon ◽

...

Keyword(s):

Avian Influenza ◽

Newcastle Disease ◽

Antibody Responses ◽

Economic Losses ◽

Cell Mediated Immunity ◽

Inactivated Vaccine ◽

Aquatic Bird ◽

Oil Emulsion ◽

Water In Oil Emulsion ◽

Newcastle Disease Vaccine

Despite the immunogenicity of vaccines currently used in poultry, several pathogens, including avian influenza virus (AIV) and Newcastle disease virus (NDV), cause enormous economic losses to the global poultry industry. The efficacy of vaccines can be improved by the introduction of effective adjuvants. This study evaluated a novel water-in-oil emulsion adjuvant, CAvant® WO-60, which effectively enhanced both the immunogenicity of conserved influenza antigen sM2HA2 and inactivated whole H9N2 antigen (iH9N2). CAvant® WO-60 induced both humoral and cell-mediated immunity in mice and provided 100% protection from challenge with 10 LD50 of A/Aquatic bird/Korea/W81/2005 (H5N2) and A/Chicken/Korea/116/2004 (H9N2) AIV. Importantly, immunization of chickens with iH9N2 plus inactivated NDV LaSota (iNDV) bivalent inactivated vaccine emulsified in CAvant® WO-60 induced seroprotective levels of antigen-specific antibody responses. Taken together, these results suggested that CAvant® WO-60 is a promising adjuvant for poultry vaccines.

Download Full-text