Исследование процесса коалесценции капель эмульсии в неоднородном переменном электрическом поле при наличии межфазной асфальтеновой пленки на границе раздела масло-вода

The behavior of a water-in-oil emulsion stabilized with asphaltenes under the action of an inhomogeneous alternating electric field has been studied. The experimental technique is based on the use of microfluidics, optical microscopy, and high-speed video filming. Quantitative estimates of the parameters characterizing the dynamics of the destruction of the emulsion, depending on the frequency and amplitude of the applied field, are obtained. The method will be useful in the development of effective methods for breaking emulsions and modifying existing technologies for separating oil emulsions into phases.

CAvant® WO-60 as an Effective Immunological Adjuvant for Avian Influenza and Newcastle Disease Vaccine

Despite the immunogenicity of vaccines currently used in poultry, several pathogens, including avian influenza virus (AIV) and Newcastle disease virus (NDV), cause enormous economic losses to the global poultry industry. The efficacy of vaccines can be improved by the introduction of effective adjuvants. This study evaluated a novel water-in-oil emulsion adjuvant, CAvant® WO-60, which effectively enhanced both the immunogenicity of conserved influenza antigen sM2HA2 and inactivated whole H9N2 antigen (iH9N2). CAvant® WO-60 induced both humoral and cell-mediated immunity in mice and provided 100% protection from challenge with 10 LD50 of A/Aquatic bird/Korea/W81/2005 (H5N2) and A/Chicken/Korea/116/2004 (H9N2) AIV. Importantly, immunization of chickens with iH9N2 plus inactivated NDV LaSota (iNDV) bivalent inactivated vaccine emulsified in CAvant® WO-60 induced seroprotective levels of antigen-specific antibody responses. Taken together, these results suggested that CAvant® WO-60 is a promising adjuvant for poultry vaccines.

Preparation and Characterization of Oil Nanoemulsion Formulations of Beauveria bassiana (Bals.-Criv.)

This study aims to prepper stable thermodynamically dilutable nanoemulsion formulation of Beauveria bassiana with the lowest surfactant concentration that could improve its solubility stability. Formulations were prepared from oil in the water nanoemulsion region of phase diagrams subjected to thermodynamic stability tests. We found propanetriol was the highest germination rate at 5% and 10% concentration, 46.66 and 53.33%, respectively. Castor oil achieved a 43.00 germination rate at 1%. Tween 80 gave 54.33 % germination rate at 10%. While Tween 20 showed a 48 % germination rate at 5%. At the concentration, 1% Term 1284 gave 43.33% rate germination. Nanoemulsion composed of propanetriol and nonionic surfactants, with a mean particle size ranging from 25.08 to 75.35 nm, was formulated for various concentrations of the oils and surfactants. Water in oil emulsion was prepared using propanetriol oil, Tween 20, Tween 80, Term 1284, and water. Nanoemulsion of 25.08, 33.75, and 75.35 nm size was obtained at a 45: 15 % ratio of oil and surfactant, and it was found to be stable. The larger droplet size 75.35 nm of formulation Tween 20 and the smaller size was 25.08 nm in the formulation of Term 1284. The higher viscosity value was 16 mPas of formulation Tween 80, and the lowest value was 7.80 in the formulation of Term 1284. To demonstrate the possible employment of these systems, they were used to formulate a nanoformulation pesticide.

Albumin Microspheres as “Trans-Ferry-Beads” for Easy Cell Passaging in Cell Culture Technology

Protein hydrogels represent ideal materials for advanced cell culture applications, including 3D-cultivation of even fastidious cells. Key properties of fully functional and, at the same time, economically successful cell culture materials are excellent biocompatibility and advanced fabrication processes allowing their easy production even on a large scale based on affordable compounds. Chemical crosslinking of bovine serum albumin (BSA) with N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) in a water-in-oil emulsion with isoparaffinic oil as the continuous phase and sorbitan monooleate as surfactant generates micro-meter-scale spherical particles. They allow a significant simplification of an indispensable and laborious step in traditional cell culture workflows. This cell passaging (or splitting) to fresh culture vessels/flasks conventionally requires harsh trypsinization, which can be omitted by using the “trans-ferry-beads” presented here. When added to different pre-cultivated adherent cell lines, the beads are efficiently boarded by cells as passengers and can be easily transferred afterward for the embarkment of novel flasks. After this procedure, cells are perfectly viable and show normal growth behavior. Thus, the trans-ferry-beads not only may become extremely affordable as a final product but also may generally replace trypsinization in conventional cell culture, thereby opening new routes for the establishment of optimized and resource-efficient workflows in biological and medical cell culture laboratories.