Fluorescence-HPLC method development and validation for the determination of Pimozide in human plasma samples

2013 ◽  
Vol 46 (12) ◽  
pp. 1154 ◽  
Author(s):  
C.A.L. Damele ◽  
F. Ghilardi ◽  
T. Celeste ◽  
A. Barassi ◽  
G. Melzi d’Eril
Keyword(s):  
Human Plasma ◽  
Method Development ◽  
Hplc Method ◽  
Plasma Samples ◽  
Human Plasma Samples ◽  
Method Development And Validation ◽  
Hplc Method Development ◽  
Development And Validation

scholarly journals Determination of Chromones in Dysophylla stellata by HPLC: Method Development, Validation and Comparison of Different Extraction Methods

2010 ◽  
Vol 5 (4) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Raju Gautam ◽  
Amit Srivastava ◽  
Sanjay M. Jachak
Keyword(s):  
Method Development ◽  
Gradient Elution ◽  
Extraction Methods ◽  
Hplc Method ◽  
Ich Guidelines ◽  
Method Development And Validation ◽  
Efficient Extraction ◽  
Hplc Method Development ◽  
Development And Validation

An HPLC method with reflux as an efficient extraction method has been developed for quantification of chromones in Dysophylla stellata Benth. Separation was achieved on a C18 column with a mobile phase of 0.5% (v/v) acetic acid in water (A), and ACN (B) under gradient elution at 1 mL/min. Chromones (1 and 2) isolated from D. stellata were used as standards for method development and validation was achieved according to ICH guidelines. Extracts prepared by three different methods [reflux, ultrasonication and accelerated solvent extraction (ASE)] were used for analysis by the validated method. The proposed HPLC method is simple, accurate and selective for the separation and quantification of chromones in D. stellata.

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