Models for the trinuclear copper(II) cluster in the particulate methane monooxygenase from methanotrophic bacteria: Synthesis, spectroscopic and theoretical characterization of trinuclear copper(II) complexes

2012 ◽  
Vol 15 (2-3) ◽  
pp. 214-224 ◽  
Author(s):  
Isiah Po-Chun Liu ◽  
Peter P.-Y. Chen ◽  
Sunney I. Chan
Keyword(s):  
Methane Monooxygenase ◽  
Methanotrophic Bacteria ◽  
Particulate Methane Monooxygenase ◽  
Theoretical Characterization

Detection of Methanotrophs in Groundwater by PCR

1999 ◽  
Vol 65 (2) ◽  
pp. 648-651 ◽  
Author(s):  
Y. S. Cheng ◽  
J. L. Halsey ◽  
K. A. Fode ◽  
C. C. Remsen ◽  
M. L. P. Collins
Keyword(s):  
Reverse Transcriptase ◽  
Environmental Samples ◽  
Methane Monooxygenase ◽  
Partial Sequence ◽  
Methanotrophic Bacteria ◽  
Particulate Methane Monooxygenase ◽  
Significant Potential ◽  
Pcr Product ◽  
Specific Amplification ◽  
Template Dna

ABSTRACT Methanotrophic bacteria have significant potential for bioremediation, which would require methods for monitoring the presence and activity of these organisms in environmental samples. In this study, PCR was used to detect methanotrophic bacteria. Primers were designed on the basis of a partial sequence of pmoA, which encodes one of the proteins of the particulate methane monooxygenase. Specific amplification of a portion of pmoA was obtained with template DNA isolated from lab strains of methanotrophs. ApmoA product was also obtained by using DNA from groundwater. The identity of the PCR product was confirmed by sequencing or by amplification with a nested primer. Reverse transcriptase PCR detected pmoA mRNA.

scholarly journals Characterization of the Particulate Methane Monooxygenase Metal Centers in Multiple Redox States by X-ray Absorption Spectroscopy

2006 ◽  
Vol 45 (20) ◽  
pp. 8372-8381 ◽  
Author(s):  
Raquel L. Lieberman ◽  
Kalyan C. Kondapalli ◽  
Deepak B. Shrestha ◽  
Amanda S. Hakemian ◽  
Stephen M. Smith ◽  
...  
Keyword(s):  
Absorption Spectroscopy ◽  
Methane Monooxygenase ◽  
Particulate Methane Monooxygenase ◽  
Metal Centers ◽  
X Ray ◽  
Redox States ◽  
X Ray Absorption

Toward Delineating the Structure and Function of the Particulate Methane Monooxygenase from Methanotrophic Bacteria†

Biochemistry ◽  
2004 ◽  
Vol 43 (15) ◽  
pp. 4421-4430 ◽  
Author(s):  
Sunney I. Chan ◽  
Kelvin H.-C. Chen ◽  
Steve S.-F. Yu ◽  
Chang-Li Chen ◽  
Simon S.-J. Kuo
Keyword(s):  
Methane Monooxygenase ◽  
Structure And Function ◽  
Methanotrophic Bacteria ◽  
Particulate Methane Monooxygenase ◽  
And Function

scholarly journals Novel methanotroph diversity evidenced by molecular characterization of particulate methane monooxygenase A (pmoA) genes in a biogas reactor

2009 ◽  
Vol 164 (5) ◽  
pp. 536-544 ◽  
Author(s):  
Gurdeep Rastogi ◽  
Dilip R. Ranade ◽  
Tulshiram Y. Yeole ◽  
Arvind K. Gupta ◽  
Milind S. Patole ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Methane Monooxygenase ◽  
Particulate Methane Monooxygenase ◽  
Biogas Reactor

scholarly journals Crystal Structure and Characterization of Particulate Methane Monooxygenase fromMethylocystisspecies Strain M

Biochemistry ◽  
2011 ◽  
Vol 50 (47) ◽  
pp. 10231-10240 ◽  
Author(s):  
Stephen M. Smith ◽  
Swati Rawat ◽  
Joshua Telser ◽  
Brian M. Hoffman ◽  
Timothy L. Stemmler ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Methane Monooxygenase ◽  
Particulate Methane Monooxygenase
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