The Effect of Melatonin On Boar Sperm Plasma Membrane Fluidity and Cryosurvival

Cryobiology ◽  
2021 ◽  
Vol 103 ◽  
pp. 190
Author(s):  
Norma A. Ramirez-Campos ◽  
Alicia Alcantar-Rodriguez ◽  
Oscar Gutierrez-Perez ◽  
Alfredo Medrano
Keyword(s):  
Plasma Membrane ◽  
Membrane Fluidity ◽  
Plasma Membrane Fluidity ◽  
Boar Sperm ◽  
Sperm Plasma Membrane

The effect of pre-freeze cooling and melatonin on pig sperm plasma membrane fluidity and cryosurvival

2019 ◽  
Vol 137 ◽  
pp. 131
Author(s):  
Alfredo Medrano ◽  
Jessica K. Orozco ◽  
Daysi A. Martinez-Hernandez ◽  
Maricela Rafael-Duran ◽  
Oscar Gutierrez-Perez ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Fluidity ◽  
Plasma Membrane Fluidity ◽  
Sperm Plasma Membrane

Plasma membrane fluidity in isolated rat hepatocytes: A comparative study using DPH and TMA-DPH as fluorescent probes

1989 ◽  
Vol 4 (3) ◽  
pp. 221-227 ◽  
Author(s):  
ANTONIO BENEDETTI ◽  
GIANNA FERRETTI ◽  
GIOVANNA CURATOLA ◽  
EUGENIO BRUNELLI ◽  
ANNE MARIE JÉZÉQUEL ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Comparative Study ◽  
Membrane Fluidity ◽  
Fluorescent Probes ◽  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Plasma Membrane Fluidity ◽  
Isolated Rat

scholarly journals Effect of Nedocromil Sodium on Polymorphonuclear Leukocyte Plasma Membrane

1994 ◽  
Vol 3 (7) ◽  
pp. S21-S24 ◽  
Author(s):  
A. Kantar ◽  
N. Oggiano ◽  
P. L. Giorgi ◽  
G. V. Coppa ◽  
R. Gabbianelli ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Steady State ◽  
Membrane Fluidity ◽  
Fluorescence Anisotropy ◽  
Polymorphonuclear Leukocyte ◽  
Polymorphonuclear Leukocytes ◽  
Plasma Membranes ◽  
Nedocromil Sodium ◽  
Plasma Membrane Fluidity ◽  
Steady State Fluorescence

The effect of nedocromil sodium on the plasma membrane fluidity of polymorphonuclear leukocytes (PMNs) was investigated by measuring steady-state fluorescence anisotropy of 1-[4-trimethylammonium-phenyl]-6-phenyl- 1,3,5-hexatriene (TMA-DPH) incorporated in the membrane. Our results show that nedocromil sodium 300 μM significantly decreased membrane fluidity of PMNs. The decrease in membrane fluidity of PMNs induced by fMLP was abolished in the presence of nedocromil sodium. These data suggest that nedocromil sodium interferes with the plasma membranes of PMNs and modulates their activities.

Erythrocyte plasma membrane fluidity in avian muscular dystrophy

1979 ◽  
Vol 64 (2) ◽  
pp. 315-326 ◽  
Author(s):  
Thomas B. Eckstein ◽  
William R. Randall ◽  
Mark G. McNamee
Keyword(s):  
Plasma Membrane ◽  
Muscular Dystrophy ◽  
Membrane Fluidity ◽  
Plasma Membrane Fluidity ◽  
Erythrocyte Plasma Membrane

Serotonin transport and fluidity in plasma membrane vesicles: effect of hyperoxia

1988 ◽  
Vol 254 (6) ◽  
pp. C781-C787 ◽  
Author(s):  
N. P. Sheridan ◽  
E. R. Block
Keyword(s):  
Endothelial Cells ◽  
Plasma Membrane ◽  
Pulmonary Artery ◽  
Membrane Fluidity ◽  
Fluorescence Probe ◽  
Membrane Vesicles ◽  
Phase System ◽  
Maximal Velocity ◽  
Plasma Membrane Vesicles ◽  
Plasma Membrane Fluidity

Plasma membrane vesicles were prepared from porcine pulmonary artery endothelial cells by a dextran-polyethylene glycol two-phase system. Specific carrier-mediated transport of 5-hydroxytryptamine (5-HT) into the vesicles was examined. Transport required a Na+ gradient (out greater than in) across the membrane, and accumulated 5-HT rapidly effluxed out of the vesicles when the ionophore gramicidin was added. Transport was inhibited by the antidepressant imipramine. 5-HT transport into plasma membrane vesicles appeared saturable and exhibited Michaelis-Menten kinetics (Km 7.4 microM, maximal velocity 217 pmol.min-1.mg membrane protein-1). A 24-h exposure to 95% O2 at 1 atmosphere absolute resulted in a 21% decrease (P less than 0.05) in specific 5-HT transport by plasma membrane vesicles. Hyperoxia also caused a significant (P less than 0.01) decrease in plasma membrane fluidity, as measured with the fluorescence probe 1,6-diphenyl-1,3,5-hexatriene. These results indicate that pulmonary artery endothelial cell plasma membrane vesicles provide a good model for studying 5-HT transport activity in vitro. Hyperoxia affects plasma membrane fluidity and 5-HT transport in pulmonary artery endothelial cells, suggesting a possible cause-and-effect relationship between the two.

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