scholarly journals Effect of Nedocromil Sodium on Polymorphonuclear Leukocyte Plasma Membrane

1994 ◽  
Vol 3 (7) ◽  
pp. S21-S24 ◽  
Author(s):  
A. Kantar ◽  
N. Oggiano ◽  
P. L. Giorgi ◽  
G. V. Coppa ◽  
R. Gabbianelli ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Steady State ◽  
Membrane Fluidity ◽  
Fluorescence Anisotropy ◽  
Polymorphonuclear Leukocyte ◽  
Polymorphonuclear Leukocytes ◽  
Plasma Membranes ◽  
Nedocromil Sodium ◽  
Plasma Membrane Fluidity ◽  
Steady State Fluorescence

The effect of nedocromil sodium on the plasma membrane fluidity of polymorphonuclear leukocytes (PMNs) was investigated by measuring steady-state fluorescence anisotropy of 1-[4-trimethylammonium-phenyl]-6-phenyl- 1,3,5-hexatriene (TMA-DPH) incorporated in the membrane. Our results show that nedocromil sodium 300 μM significantly decreased membrane fluidity of PMNs. The decrease in membrane fluidity of PMNs induced by fMLP was abolished in the presence of nedocromil sodium. These data suggest that nedocromil sodium interferes with the plasma membranes of PMNs and modulates their activities.

scholarly journals A study of the Interaction Between Cetirizine and Plasma Membrane of Eosinophils, Neutrophils, Platelets and Lymphocytes using A fluorescence Technique

1994 ◽  
Vol 3 (3) ◽  
pp. 229-234 ◽  
Author(s):  
A. Kantar ◽  
N. Oggiano ◽  
P. L. Giorgi ◽  
J-P. Rihoux
Keyword(s):  
Plasma Membrane ◽  
Membrane Fluidity ◽  
Fluorescence Anisotropy ◽  
Inflammatory Cell ◽  
Fluorescence Decay ◽  
Fluorescence Technique ◽  
Membrane Heterogeneity ◽  
Plasma Membrane Fluidity ◽  
Steady State Fluorescence ◽  
Induced Changes

The effect of cetirizine on plasma membrane fluidity and heterogeneity of human eosinophils, neutrophils, platelets and lymphocytes was investigated using a fluorescence technique. Membrane fluidity and heterogeneity were studied by measuring the steady-state fluorescence anisotropy and fluorescence decay of 1-(4- trimethylammonium-phenyl)-6-phenyl-1, 3, 5-hexatriene (TMA-DPH) incorporated in the membrane. The results demonstrate that cetirizine (1 μg/ml) induced a significant increase in the Hpid order in the exterior part of the membrane and a decrease in membrane heterogeneity in eosinophils, neutrophils and platelets. Moreover, cetirizine blocked the PAF induced changes in membrane fluidity in these cells. Cetirizine did not influence significantly the plasma membrane of lymphocytes. These data may partially explain the effect ofcetirizine on inflammatory cell activities.

Plasma Membrane Fluidity and Polarity of Polymorphonuclear Leukocytes from Children with Type I Diabetes Mellitus

1999 ◽  
Vol 13 (5-6) ◽  
pp. 243-250 ◽  
Author(s):  
Ahmad Kantar ◽  
Gian Paolo Littarru ◽  
Giancarlo Falcioni ◽  
Valentino Cherubini ◽  
Giovanni Valentino Coppa ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Plasma Membrane ◽  
Membrane Fluidity ◽  
Polymorphonuclear Leukocytes ◽  
Type I Diabetes ◽  
Type I Diabetes Mellitus ◽  
Type I ◽  
Plasma Membrane Fluidity

scholarly journals Effect of PAF on polyrnorphonuclear leucocyte plasma membrane polarity: a fluorescence study

1993 ◽  
Vol 2 (2) ◽  
pp. 149-151 ◽  
Author(s):  
A. Kantar ◽  
P. L. Giorgi ◽  
R. Fiorini
Keyword(s):  
Plasma Membrane ◽  
Steady State ◽  
Polymorphonuclear Leukocytes ◽  
Fluorescence Emission ◽  
Emission Spectra ◽  
Blue Shift ◽  
Fluorescence Study ◽  
Polar Solvents ◽  
Fluorescence Emission Spectra ◽  
Steady State Fluorescence

The effect of PAF on the plasma membrane polarity of polymorphonuclear leukocytes (PMNs) was investigated by measuring the steady-state fluorescence emission spectra of 2-dimethylamino(6-1auroyl) naphthalene (Laurdan), which is known to be incorporated at the hydrophobic-hydrophilic interface of the bilayer, displaying spectral sensitivity to the polarity of its surrounding. Laurdan shows a marked steady-state emission blue-shift in non-polar solvents, with respect to polar solvents. Our results demonstrate that PAF (10−7M) induces a blue shift of the fluorescence emission spectra of Laurdan. These changes are blocked in the presence of the PAF antagonist, L-659,989. Our data indicate that the interaction between PAF and PMNs is accompanied by a decrease in polarity in the hydrophobic-hydrophilic interface of the plasma membrane.

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