Standardizing the Cryopreservation Procedure of European Perch Semen for the Development of Consistent Procedures and Future Implementation of Cryopreservation Technology in Commercial Hatcheries

Eustrongylides spp. is considered a freshwater fish zoonotic nematode. In the present study, the prevalence of Eustrongylides spp. in six edible fish (European perch - Perca fluviatilis, goldfish - Carassius auratus, largemouth black bass - Micropterus salmoides, tench- Tinca tinca, carp - Cyprinus carpio and sand smelt - Atherina boyeri) of Trasimeno lake was surveyed. The investigations were conducted from October 2014 to September 2015 and 384 specimens per species for each season were caught in Trasimeno lake and examined for the presence of larvae in the abdominal cavity and muscle. The presence of nematodes in the abdominal cavity and musculature was revealed in three fish species. The prevalence of Eustrongylides spp. infection was 6.84, 1.89 and 0.13% in perch, largemouth black bass and sand smelt, respectively. The number of parasites per fish was only one in largemouth black bass and sand smelt and ranged from one up to three in perch. This study states that the European perch, largemouth black bass and sand smelt of Trasimeno lake are infected with zoonotic parasites; therefore, food business operators have to take appropriate measures to guarantee the health of consumers.

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Aeromonas salmonicida infected fish transfer disease to healthy fish via water

Open Life Sciences ◽

10.2478/s11535-012-0066-8 ◽

2012 ◽

Vol 7 (5) ◽

pp. 878-885

Author(s):

Vesta Skrodenytė-Arbačiauskienė ◽

Nijolė Kazlauskienė ◽

Milda Vosylienė ◽

Tomas Virbickas

Keyword(s):

Blood Cell ◽

Experimental Studies ◽

Infected Fish ◽

Aeromonas Salmonicida ◽

Internal Organs ◽

European Perch ◽

Infection Transmission ◽

Cell Parameters ◽

Sea Trout ◽

Tryptone Soya Agar

AbstractExperimental studies of infection transmission via water from infected to healthy fish were conducted. The dark-brown bacterial colonies typical for Aeromonas salmonicida on tryptone soya agar (TSA) have been isolated and counted (from 3.0±0.6×102 to 3.5±0.5×105 c.f.u. g−1) from the internal organs of naturally infected (NI) and experimentally infected (EI) perch and sea trout. No significant differences in dark-brown bacterial counts were detected between EI perch and EI sea trout. The assessment and comparison of the alterations of the biological parameters of EI European perch and sea trout with bacterium Aeromonas salmonicida subsp. salmonicida with naturally infected perch were conducted. No mortality was recorded in groups of EI perch and sea trout. Whereas, the mortality of NI perch (collected from the main sites of outbreak of disease) was observed from the second day of the experiments. Changes in morphophysiological parameters of EI perch and sea trout were similar. Different alterations in blood cell parameters of EI fish were observed, and the most noticeable was the decrease (P≤0.01) in white blood cell count (WBC) of EI perch and sea trout. Based on these results it can be deduced that there is infection transmission of bacterium A. salmonicida from European perch via water to other fish species.

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Adult female European perch (Perca fluviatilis) from the Baltic Sea show no evidence of thiamine deficiency

Journal of Sea Research ◽

10.1016/j.seares.2021.102081 ◽

2021 ◽

pp. 102081

Author(s):

Johan Gustafsson ◽

Karin Ström ◽

Linus Arvstrand ◽

Lars Förlin ◽

Lillemor Asplund ◽

...

Keyword(s):

Baltic Sea ◽

Adult Female ◽

Thiamine Deficiency ◽

Perca Fluviatilis ◽

The Baltic Sea ◽

European Perch ◽

Perch Perca Fluviatilis ◽

The Baltic

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Certification of European perch from the Irikla Reservoir (basin of the Ural River) according to the standards of the Marine Stewardship Council

Trudy VNIRO ◽

10.36038/2307-3497-2020-179-124-148 ◽

2020 ◽

Vol 179 ◽

pp. 124-148

Author(s):

D. L. Lajus ◽

I. A. Belyanin ◽

E. P. Ermolova ◽

P. V. Golovin

Keyword(s):

Fisheries Management ◽

Perca Fluviatilis ◽

World Market ◽

Small Scale ◽

European Perch ◽

Certification Programs ◽

Marine Stewardship Council ◽

Depth Analysis ◽

Seafood Products ◽

Reservoir Basin

Certification according to the standards of the Marine Stewardship Council (MSC) is highly demanded in the world market of seafood products. In Russia, a number of fisheries already have MSC certificates, but still there is a significant potential for certification of other fisheries. This requires analysis of the experience of companies that have successfully obtained the certification. Special attention should be paid to small-scale fisheries, whose financial opportunities to participate in certification programs, unlike large fisheries, are seriously limited. In the present work, process of ecological certification of fishery is considered on an example of the gillnet fishery of European perch Perca fluviatilis in the Irikla Reservoir, located in the middle course of the river Ural in the Orenburg Province. The catch volume of perch is 200–250 metric tons during the recent years. Fishing companies that fish in this reservoir have been working on MSC certification for over ten years. Process of certification has shown that the fishery meets the necessary standards, but even after the certification completed in 2016, there is a need for certain activities to support certification. These are in-depth analysis of the recreational fisheries, development of research plans in cooperation with scientific fishery institutes, specifically aimed at informing the certification process, independent analysis of the fisheries management system, detailed study of various bycatch species. It can be concluded that positive experience of this small-scale fishery with ecological certification can be important in improving fisheries management not only in Russia but also worldwide.

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Factors determining parasite abundance in European perch, Perca fluviatilis, European whitefish, Coregonus lavaretus, and Arctic charr, Salvelinus alpinus, in an oligotrophic lake, southern Norway

Fauna norvegica ◽

10.5324/fn.v40i0.3444 ◽

2020 ◽

Vol 40 ◽

pp. 109-129

Author(s):

Tom Robin Olk ◽

Ann-Cecilie Henriksen ◽

Solveig Irene Dolven ◽

Mathias Leithe Haukø ◽

Espen Lydersen ◽

...

Keyword(s):

Salvelinus Alpinus ◽

Arctic Charr ◽

Perca Fluviatilis ◽

Coregonus Lavaretus ◽

Host Age ◽

European Perch ◽

Parasite Abundance ◽

Perch Perca Fluviatilis ◽

Southern Norway ◽

Benthic Animals

Time and spatial variations in macroparasite status were investigated in European perch, Perca fluviatilis, European whitefish, Coregonus lavaretus, and Arctic charr, Salvelinus alpinus in Lake Norsjø (Southern Norway), based on gillnet fishing in three locations in the spring, summer, and fall 2018. In addition, length, weigth, age, sex, δ13C, and δ15N were determined. Parasite abundance was modelled using negative binomial generalized linear models in relation to fish metrics, season, and sampling location. The most prevalent parasite species were determined by the diet and habitat of the host. European perch was mainly infected by acanthocephalans, European whitefish mainly infected by acanthocephalans and cestodes, and Arctic charr mainly infected by cestodes. The most prevalent parasites in European perch are transmitted by benthic animals. Parasites in European whitefish are transmitted by both benthic animals and copepods, while the most prevalent parasites in Arctic charr are copepod transmitted. This corresponds well with the δ13C signatures in the three species, indicating that European perch primarily fed in the littoral zone (δ13C: -24.9 ± 2.5 ‰), Arctic charr in the pelagic and profundal zone (δ13C: -29.4 ± 1.1 ‰), while European whitefish both fed in the littoral and pelagic zone (δ13C: -28.3 ± 2.3 ‰) of Lake Norsjø. Individual abundances of parasites depended on host age, length, sex, δ15N, and season. Positive correlations between parasite abundance and host age, length, or trophic level measured as δ15N were most common, and occurred in all three host species. Many parasites accumulate with age, and larger hosts provide more diverse habitats for parasites.

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Environmental DNA monitoring of noble crayfish Astacus astacus: Comparison and refining of methodology

ARPHA Conference Abstracts ◽

10.3897/aca.4.e65010 ◽

2021 ◽

Vol 4 ◽

Author(s):

David Strand ◽

Stein Johnsen ◽

Frode Fossøy ◽

Johannes Rusch ◽

Brett Sandercock ◽

...

Keyword(s):

Dna Extraction ◽

Brown Trout ◽

Detection Probability ◽

Environmental Dna ◽

Northern Pike ◽

Freshwater Crayfish ◽

Astacus Astacus ◽

European Perch ◽

Detection Frequency ◽

Noble Crayfish

During the past decade, environmental DNA (eDNA) methodology has become an important non-invasive tool to monitor aquatic micro- and macro-organisms, including freshwater crayfish. In Europe, noble crayfish Astacus astacus is the most widespread native freshwater crayfish. However, the species is threatened in its entire distribution range. It is therefore included on the International Union for Conservation Nature (IUCN) red list, and on several national red lists. Reliable monitoring is essential for implementation of conservation measures. For crayfish, traditional population trends have been obtained from catch per unit effort (CPUE) data. In order to successfully apply and use eDNA monitoring for noble crayfish, or any species, it is a prerequisite to know the strengths and weaknesses of the applied methods and how they perform compared to traditional methodology. Sampling strategy and analysis methodology also depends on choice of species to be monitored, and which questions to be answered. Further, refinement of the employed methods may improve the detection probability for eDNA monitoring. Here we report the results from 1) a recently published study on noble crayfish eDNA monitoring (Johnsen et al. 2020) and 2) an ongoing study comparing and optimising the methods used for monitoring noble crayfish. 1) We compared eDNA monitoring (transects with ten 5L samples) with traditional trapping (transects with 50 traps) for noble crayfish in lentic habitats, in order to evaluate detection probability and if eDNA concentration correlates with relative density of crayfish. We also compared two commonly used analytical methods [quantitative real-time PCR (qPCR) and droplet digital PCR (ddPCR)] for eDNA monitoring. We found that qPCR outperformed ddPCR in detection frequency (Fig. 1), most likely due to some inhibition in the ddPCR analysis. eDNA monitoring provided reliable presence/absence data for noble crayfish, even in lakes with very low crayfish densities. Detection frequency increased with increasing CPUE (Fig. 1). However, we did not observe any correlation between relative crayfish densities and eDNA concentrations of crayfish. eDNA concentrations were consistently very low, even in lakes with very high crayfish densities. For lakes with very low crayfish densities, we estimated that ~5 samples (5L samples) are needed for 95 % detection likelihood, while for lakes with high densities 2 samples were needed. 2) We compared two eDNA sampling strategies (sampling from bottom or the surface), commonly used for crayfish or fish in Norway to investigate how both strategies perform. The sampled filters were divided and two DNA extraction protocols were evaluated (CTAB based vs Column based). We found that the DNA yield was higher from the column based DNA extraction protocol, and that eDNA concentrations from fish (brown trout Salmon trutta, northern pike Esox lucius and European perch Perca fluviatilis) were significantly higher than for crayfish. For crayfish and brown trout, there was little difference between detection probability for bottom and surface samples, while for northern pike and European perch the detection probability was higher for the bottom samples. Currently, we are analysing eDNA samples collected with glass fibre filters and NatureMetrix filters for noble crayfish in both lentic and lotic habitats and the preliminary results will be presented. We conclude that eDNA monitoring cannot substitute CPUE monitoring for freshwater crayfish, but it offers reliable presence-absence data, provided sufficient sampling efforts. Thus, it is suitable for large scale monitoring of threatened crayfish and combined with eDNA analysis of alien crayfish and diseases such as crayfish plague, this is a cost-efficient supplement offering a more holistic approach for aquatic environments and native crayfish conservation. Furthermore, the synergy effect of using collected eDNA samples from different projects to monitor additional species is substantial.

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Risk-sensitive mating decisions in a visually compromised environment

Biology Letters ◽

10.1098/rsbl.2009.0350 ◽

2009 ◽

Vol 5 (5) ◽

pp. 600-602 ◽

Cited By ~ 9

Author(s):

Bob B. M. Wong ◽

Marja Järvenpää ◽

Kai Lindström

Keyword(s):

Visual Cues ◽

Perca Fluviatilis ◽

Reproductive Decisions ◽

Sand Goby ◽

European Perch ◽

Pomatoschistus Minutus ◽

Risk Sensitive ◽

Risk Of Predation ◽

Males And Females ◽

Reproductive Activities

Reproductive activities are often conspicuous and can increase the risk of predation. Evidence suggests that individuals are capable of responding to predators in a risk-sensitive manner. However, most studies tend to consider only the predator-mediated responses of males and females in isolation and with little regard to differences in local environmental conditions. Here, we experimentally investigate the effects of environmental visibility (turbidity) and predation risk on reproductive decisions in the sand goby, Pomatoschistus minutus , when exposed to a visually oriented predator, the European perch, Perca fluviatilis . We found that gobies were more reluctant to spawn in the predator's presence, although larger males spawned sooner than smaller males. Interestingly, latency to spawning was unaffected by the visual environment, suggesting that gobies may be relying on non-visual cues under turbid conditions.

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Monitoring of the ecological state of the city lake of Chita

Safety of technogenic and natural systems ◽

10.23947/2541-9129-2021-4-52-57 ◽

2021 ◽

pp. 52-57

Author(s):

Vladimir V. Zvyagintsev ◽

Olga Yu. Zvyagintseva ◽

Vadim K. Chernyshov

Keyword(s):

Heavy Metals ◽

Fluctuating Asymmetry ◽

Aquatic Ecosystem ◽

Perca Fluviatilis ◽

Gibel Carp ◽

European Perch ◽

Lake Kenon ◽

Perch Perca Fluviatilis ◽

The City

Introduction. The paper presents the results of the studies on assessing the quality of the aquatic ecosystem of a lake located within the city. The European perch (Perca fluviatilis L.) and the Gibel carp (Carassius gibelio) were used as an indicator for determining the quality of the aquatic ecosystem by the method of fluctuating asymmetry. Problem Statement. The aim of the work was to conduct monitoring with the subsequent assessment of the quality of the ecosystem of the city lake using the method of fluctuating asymmetry (hereinafter FA). Theoretical and Practical Part. The quality of the urban lake aquatic ecosystem was assessed using the FA method (indicators: the European perch (Perca fluviatilis L.), 1758 and the Gibel carp (Carassius auratus Bloch), 1783). To identify the reasons for the high PFA values, a chemical analysis (in an accredited laboratory) of the gills of the Gibel carp for heavy metals was performed. Conclusions. As a result of the research, the FA indicators values for these indicators were obtained. According to the results of a laboratory study of the content of heavy metals in the gill arches of the Gibel carp, an excess of the maximum permissible concentration of 8 out of 10 analyzed elements was revealed. It has been established that the ecosystem of the city lake Kenon is experiencing a significant anthropogenic load (5 points — the critical quality of the aquatic environment) and it continues to increase towards the deterioration of the habitat.

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