scholarly journals Bypassing the Diffraction Barrier

2014 ◽  
Vol 31 (5) ◽  
pp. 513-516
Keyword(s):  
Diffraction Barrier

scholarly journals Breaking the Diffraction Barrier in Fluorescence Microscopy by Optical Shelving

2007 ◽  
Vol 98 (21) ◽  
Author(s):  
Stefan Bretschneider ◽  
Christian Eggeling ◽  
Stefan W. Hell
Keyword(s):  
Fluorescence Microscopy ◽  
Diffraction Barrier

Breaking the diffraction barrier

2016 ◽  
Vol 13 (4) ◽  
pp. 286-287
Author(s):  
Allison Doerr
Keyword(s):  
Diffraction Barrier

Imaging the invisible: resolving cellular microcompartments by superresolution microscopy techniques

2013 ◽  
Vol 394 (9) ◽  
pp. 1097-1113 ◽  
Author(s):  
Michael Hensel ◽  
Jürgen Klingauf ◽  
Jacob Piehler
Keyword(s):  
Imaging Techniques ◽  
Live Cell ◽  
Temporal Organization ◽  
Membrane Microdomains ◽  
Superresolution Microscopy ◽  
Neuronal Synapses ◽  
Spatio Temporal ◽  
Microscopy Techniques ◽  
Plasma Membrane Microdomains ◽  
Diffraction Barrier

Abstract Unraveling the spatio-temporal organization of dynamic cellular microcompartments requires live cell imaging techniques capable of resolving submicroscopic structures. While the resolution of traditional far-field fluorescence imaging techniques is limited by the diffraction barrier, several fluorescence-based microscopy techniques providing sub-100 nm resolution have become available during the past decade. Here, we briefly introduce the optical principles of these techniques and compare their capabilities and limitations with respect to spatial and temporal resolution as well as live cell capabilities. Moreover, we summarize how these techniques contributed to a better understanding of plasma membrane microdomains, the dynamic nanoscale organization of neuronal synapses and the sub-compartmentation of microorganisms. Based on these applications, we highlight complementarity of these techniques and their potential to address specific challenges in the context of dynamic cellular microcompartments, as well as the perspectives to overcome current limitations of these methods.

scholarly journals A Colorful New Way to Look at the Nuclear Pore!

2009 ◽  
Vol 17 (2) ◽  
pp. 3-5
Author(s):  
Stephen W. Carmichael
Keyword(s):  
Light Microscopy ◽  
Super Resolution ◽  
Nuclear Pore ◽  
Conventional Methods ◽  
Diffraction Barrier

Light microscopy has many advantages and several disadvantages. One of the advantages is that different wavelengths are perceived as different colors, and with the proper use of dyes, filters, etc. several different structures can be imaged in the same object. One the major disadvantages is that the resolution is limited by the wavelength, a limit known as the diffraction barrier. Several recently-developed techniques have allowed light microscopy to “break” the diffraction barrier, a phenomenon known as “super-resolution.” Lothar Schermelleh, Peter Carlton, Sebastian Haase, Lin Shao, Lukman Winoto, Peter Kner, Brian Burke, Cristina Cardoso, David Agard, Mats Gustafsson, Heinhrich Leonardt, and John Sedat have created a new microscope that not only achieves super-resolution, but creates images in 3 dimensions and multiple colors. Not only that, but preparing specimens to examine with this microscope uses conventional methods, and they claim the microscope is easy to use!

Fluorescence nanoscopy: Breaking the diffraction barrier by the RESOLFT concept

2005 ◽  
Vol 1 (3) ◽  
pp. 296-297 ◽  
Author(s):  
Stefan W. Hell
Keyword(s):  
Fluorescence Nanoscopy ◽  
Diffraction Barrier

MOLECULAR IMAGING: New Optics Strategies Cut Through Diffraction Barrier

Science ◽  
2006 ◽  
Vol 313 (5788) ◽  
pp. 748a-749a
Author(s):  
J. Couzin
Keyword(s):  
Molecular Imaging ◽  
Diffraction Barrier

scholarly journals Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins

2005 ◽  
Vol 102 (49) ◽  
pp. 17565-17569 ◽  
Author(s):  
M. Hofmann ◽  
C. Eggeling ◽  
S. Jakobs ◽  
S. W. Hell
Keyword(s):  
Fluorescence Microscopy ◽  
Low Light ◽  
Light Intensities ◽  
Diffraction Barrier

scholarly journals Imaging with the Photon Scanning-Tunneling Microscope

1999 ◽  
Vol 7 (4) ◽  
pp. 14-17
Author(s):  
Thomas L. Ferrell ◽  
Fabrice Meriaudeau ◽  
Ali Passian ◽  
Jean-Pierre Goudonnet ◽  
Andrew Wig
Keyword(s):  
Scanning Tunneling Microscope ◽  
Albert Einstein ◽  
Diffraction Limit ◽  
Significant Fraction ◽  
Scanning Tunneling ◽  
Tunneling Microscope ◽  
The Past ◽  
Central Maximum ◽  
Photon Scanning Tunneling Microscope ◽  
Diffraction Barrier

The use of traditional optics in microscopy has a well-known resolution barrier first presented by Lord Rayleigh. This is the "Rayleigh diffraction limit." In the usual textbook example, the overlapping diffraction rings from two small objects are set so that the central maximum of one pattern falls on the first minimum of the second, The barrier limits the resolution of conventional microscopes to a significant fraction of the wavelength of light.In the past decade two methods were discovered for surpassing the Rayleigh diffraction barrier. The first was actually an independent rediscovery by D. Pohl er al. of a concept originally proposed by Synge (in a 1928 paper reviewed by Albert Einstein),

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