Determination of in vitro susceptibility of ocular Fusarium spp. isolates from keratitis cases and comparison of Clinical and Laboratory Standards Institute M38-A2 and E test methods

2008 ◽  
Vol 62 (3) ◽  
pp. 348-350 ◽  
Author(s):  
Naureen J. Iqbal ◽  
Angeline Boey ◽  
Benjamin J. Park ◽  
Mary E. Brandt
Keyword(s):  
Test Methods ◽  
Fusarium Spp ◽  
In Vitro Susceptibility

scholarly journals Risk Factors for the Antibiotic Resistant Gram-Negative Bacilli Associated Infections in Burn Patients and the In-Vitro Susceptibility of Colistin

2020 ◽  
Vol 15 (3) ◽  
Author(s):  
Mojtaba Varshochi ◽  
Alka Hasani ◽  
Parinaz Pour Shahverdi ◽  
Fateme Ravanbakhsh Ghavghani ◽  
Somaieh Matin
Keyword(s):  
Risk Factors ◽  
Infectious Complications ◽  
Test Methods ◽  
Burn Patients ◽  
In Vitro Susceptibility ◽  
Antibiotic Resistant ◽  
Gram Negative ◽  
Burn Unit ◽  
Microbial Infections

Background: Burns patients are predisposed to infectious complications. Amongst microbial infections, Gram-negative bacilli are the most prevalent bacteria in the burn units. Objectives: The current study aimed to identify the risk factors associated with antibiotic-resistant Gram-negative bacilli in hospitalized burn patients and determine the in-vitro susceptibility of these organisms to colistin. Methods: Two hundred burn patients hospitalized in the burn unit and ICU burn ward were allocated to two groups (each with 100 patients) of patients with antibiotic-resistant Gram-negative bacilli infections and the other with antibiotic susceptible Gram-negative bacilli associated infections. The susceptibility of Gram-negative bacilli was done towards various antibacterial agents by the Kirby-Bauer method. Susceptibility of colistin was performed using both E-test and disc diffusion methods. Results: The history of antibiotic usage, length of ICU stay, mechanical ventilation, and catheter usage were the most important risk factors for infections associated with antibiotic-resistant Gram-negative bacilli. Pseudomonas aeruginosa and Acinetobacter baumannii were the most prevalent bacteria in the burn unit. Only one A. baumannii isolate was found resistant toward colistin by both disk diffusion and E-test methods. Conclusions: Burn patients are prone to infections, and Gram-negative bacilli predominates in patients harboring risk factors. These findings influence the choice of traditional therapeutic regimens in such patients. Colistin served as an appropriate antibiotic choice.

scholarly journals In vitro susceptibility testing of Fonsecaea pedrosoi to antifungals

1997 ◽  
Vol 39 (3) ◽  
pp. 145-148 ◽  
Author(s):  
Catalina de BEDOUT ◽  
Beatriz L. GÓMEZ ◽  
Angela RESTREPO
Keyword(s):  
Amphotericin B ◽  
Susceptibility Testing ◽  
Significant Proportion ◽  
Clinical Failure ◽  
Fonsecaea Pedrosoi ◽  
In Vitro Susceptibility ◽  
In Vitro Susceptibility Testing

Based on the difficulties experienced in the treatment of chromoblastomycosis, 12 primary human isolates of F. pedrosoi, were tested for their in vitro susceptibility to various antimycotics. We adapted the recommendations of the NCCLS for yeasts and followed the indications for mold testing from other authors in order to determine their MIC’s and the MLC’s. It was found that a significant proportion of the isolates were resistant to 3 of the 4 antimycotics tested, as revealed by high MIC values, as follows: 33% were resistant to amphotericin B (AMB), 58.3% to 5 fluocytosine (5 FC) and 66.7% to fluconazole (FLU). Contrarywise, none of the isolates proved resistant to itraconazole (ITZ). Determination of the MLC’s revealed that a larger proportion of the isolates were not killed by AMB, 5 FC (91.7%), FLU (100%) or even, ITZ (41.7%). These data indicate that it would be desirable to determine the susceptibility of F. pedrosoi before initiating therapy, in order to choose the more effective antifungal and avoid clinical failure

In vitro Determination of Fusarium spp. Infection on Watermelon Seeds and their Localization

2006 ◽  
Vol 5 (2) ◽  
pp. 178-182 ◽  
Author(s):  
N. Boughalleb ◽  
M. El Mahjoub .
Keyword(s):  
Fusarium Spp

scholarly journals Determination of Disk Diffusion and MIC Quality Control Guidelines for High-Dose Cefepime-Tazobactam (WCK 4282), a Novel Antibacterial Combination Consisting of a β-Lactamase Inhibitor and a Fourth-Generation Cephalosporin

2017 ◽  
Vol 55 (10) ◽  
pp. 3130-3134 ◽  
Author(s):  
Stefan Riedel ◽  
Michael D. Huband ◽  
Helio S. Sader ◽  
Robert K. Flamm ◽  
Ronald N. Jones
Keyword(s):  
Quality Control ◽  
Generation Cephalosporin ◽  
Test Methods ◽  
Disk Diffusion ◽  
Fourth Generation ◽  
High Dose ◽  
Gram Negative ◽  
Lactamase Inhibitor

ABSTRACTHigh-dose cefepime-tazobactam (1:1; WCK 4282), a novel antibacterial combination consisting of the β-lactamase inhibitor tazobactam and a fourth-generation cephalosporin, is under clinical development for the treatment of serious Gram-negative infections. A quality control (QC) study was performed to establish disk diffusion and MIC ranges for cefepime-tazobactam for multiple QC reference strains. The cefepime-tazobactam QC ranges for a fixed tazobactam MIC of 8 μg/ml and disk diffusion (30/20-μg disk) test methods were approved by the CLSI Subcommittee on Antimicrobial Susceptibility Testing in January 2015 and January 2016. These QC ranges will be important for accuratein vitroactivity evaluations of cefepime-tazobactam when tested against clinical Gram-negative bacteria during clinical studies and routine patient care.

Sign in / Sign up

Export Citation Format

Share Document