Real-time Next-Generation Sequencing on Shell-vial Culture to contribute to diagnosis of lymphatic tuberculosis: a case report.

2021 ◽  
pp. 115492
Author(s):  
Madjid Morsli ◽  
Marc Faltot ◽  
Hélène Astier ◽  
Erwan Le Dault ◽  
Bernard Chaudier ◽  
...  
Keyword(s):  
Case Report ◽  
Next Generation Sequencing ◽  
Real Time ◽  
Next Generation ◽  
Shell Vial ◽  
Generation Sequencing

scholarly journals Case Report: Next generation sequencing identifies a NAB2-STAT6 fusion in Glioblastoma

2016 ◽  
Vol 11 (1) ◽  
Author(s):  
Phedias Diamandis ◽  
Ruben Ferrer-Luna ◽  
Raymond Y. Huang ◽  
Rebecca D. Folkerth ◽  
Azra H. Ligon ◽  
...  
Keyword(s):  
Case Report ◽  
Next Generation Sequencing ◽  
Next Generation ◽  
Generation Sequencing

scholarly journals Haemophilus influenzae Meningitis Direct Diagnosis by Metagenomic Next-Generation Sequencing: A Case Report

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 461
Author(s):  
Madjid Morsli ◽  
Quentin Kerharo ◽  
Jeremy Delerce ◽  
Pierre-Hugues Roche ◽  
Lucas Troude ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Haemophilus Influenzae ◽  
Real Time ◽  
Real Time Pcr ◽  
Point Of Care ◽  
Next Generation ◽  
Oxford Nanopore ◽  
Sequence Encoding ◽  
Oxford Nanopore Technologies ◽  
Generation Sequencing

Current routine real-time PCR methods used for the point-of-care diagnosis of infectious meningitis do not allow for one-shot genotyping of the pathogen, as in the case of deadly Haemophilus influenzae meningitis. Real-time PCR diagnosed H. influenzae meningitis in a 22-year-old male patient, during his hospitalisation following a more than six-metre fall. Using an Oxford Nanopore Technologies real-time sequencing run in parallel to real-time PCR, we detected the H. influenzae genome directly from the cerebrospinal fluid sample in six hours. Furthermore, BLAST analysis of the sequence encoding for a partial DUF417 domain-containing protein diagnosed a non-b serotype, non-typeable H.influenzae belonging to lineage H. influenzae 22.1-21. The Oxford Nanopore metagenomic next-generation sequencing approach could be considered for the point-of-care diagnosis of infectious meningitis, by direct identification of pathogenic genomes and their genotypes/serotypes.

P034 Next generation sequencing - the ultimate tool for confirmatory HLA typing in cadaveric donors? A case report

2019 ◽  
Vol 80 ◽  
pp. 80
Author(s):  
Ibis T. Hernandez ◽  
Jennifer McCue ◽  
Ana Hernandez ◽  
Ozzarah Ferrer ◽  
Maria Reiger ◽  
...  
Keyword(s):  
Case Report ◽  
Next Generation Sequencing ◽  
Hla Typing ◽  
Next Generation ◽  
Cadaveric Donors ◽  
Generation Sequencing
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