Nucleotide excision repair deficiency causes elevated levels of chromosome gain in Saccharomyces cerevisiae

Abstract The Saccharomyces cerevisiae homologs of the bacterial mismatch repair proteins MutS and MutL correct replication errors and prevent recombination between homeologous (nonidentical) sequences. Previously, we demonstrated that Msh2p, Msh3p, and Pms1p regulate recombination between 91% identical inverted repeats, and here use the same substrates to show that Mlh1p and Msh6p have important antirecombination roles. In addition, substrates containing defined types of mismatches (base-base mismatches; 1-, 4-, or 12-nt insertion/deletion loops; or 18-nt palindromes) were used to examine recognition of these mismatches in mitotic recombination intermediates. Msh2p was required for recognition of all types of mismatches, whereas Msh6p recognized only base-base mismatches and 1-nt insertion/deletion loops. Msh3p was involved in recognition of the palindrome and all loops, but also had an unexpected antirecombination role when the potential heteroduplex contained only base-base mismatches. In contrast to their similar antimutator roles, Pms1p consistently inhibited recombination to a lesser degree than did Msh2p. In addition to the yeast MutS and MutL homologs, the exonuclease Exo1p and the nucleotide excision repair proteins Rad1p and Rad10p were found to have roles in inhibiting recombination between mismatched substrates.

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Photolyases from Saccharomyces cerevisiae and Escherichia coli recognize common binding determinants in DNA containing pyrimidine dimers.

Molecular and Cellular Biology ◽

10.1128/mcb.9.11.4777 ◽

1989 ◽

Vol 9 (11) ◽

pp. 4777-4788 ◽

Cited By ~ 23

Author(s):

M Baer ◽

G B Sancar

Keyword(s):

Escherichia Coli ◽

Saccharomyces Cerevisiae ◽

Nucleotide Excision Repair ◽

Excision Repair ◽

Spectroscopic Studies ◽

Pyrimidine Dimer ◽

Nucleotide Sequence Analysis ◽

Binding Motif ◽

Pyrimidine Dimers ◽

Nucleotide Excision

DNA photolyases catalyze the light-dependent repair of pyrimidine dimers in DNA. The results of nucleotide sequence analysis and spectroscopic studies demonstrated that photolyases from Saccharomyces cerevisiae and Escherichia coli share 37% amino acid sequence homology and contain identical chromophores. Do the similarities between these two enzymes extend to their interactions with DNA containing pyrimidine dimers, or does the organization of DNA into nucleosomes in S. cerevisiae necessitate alternative or additional recognition determinants? To answer this question, we used chemical and enzymatic techniques to identify the contacts made on DNA by S. cerevisiae photolyase when it is bound to a pyrimidine dimer and compared these contacts with those made by E. coli photolyase and by a truncated derivative of the yeast enzyme when bound to the same substrate. We found evidence for a common set of interactions between the photolyases and specific phosphates in the backbones of both strands as well as for interactions with bases in both the major and minor grooves of dimer-containing DNA. Superimposed on this common pattern were significant differences in the contributions of specific contacts to the overall binding energy, in the interactions of the enzymes with groups on the complementary strand, and in the extent to which other DNA-binding proteins were excluded from the region around the dimer. These results provide strong evidence both for a conserved dimer-binding motif and for the evolution of new interactions that permit photolyases to also act as accessory proteins in nucleotide excision repair. The locations of the specific contacts made by the yeast enzyme indicate that the mechanism of nucleotide excision repair in this organism involves incision(s) at a distance from the pyrimidine dimer.

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A Synthetic Interaction between CDC20 and RAD4 in Saccharomyces cerevisiae upon UV Irradiation

Molecular Biology International ◽

10.1155/2014/519290 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8

Author(s):

Bernadette Connors ◽

Lauren Rochelle ◽

Asela Roberts ◽

Graham Howard

Keyword(s):

Saccharomyces Cerevisiae ◽

Nucleotide Excision Repair ◽

Uv Irradiation ◽

Double Mutant ◽

Excision Repair ◽

Strand Break ◽

Anaphase Promoting Complex ◽

End Joining ◽

Ubiquitin Ligase Complex ◽

Nucleotide Excision

Regulation of DNA repair can be achieved through ubiquitin-mediated degradation of transiently induced proteins. In Saccharomyces cerevisiae, Rad4 is involved in damage recognition during nucleotide excision repair (NER) and, in conjunction with Rad23, recruits other proteins to the site of damage. We identified a synthetic interaction upon UV exposure between Rad4 and Cdc20, a protein that modulates the activity of the anaphase promoting complex (APC/C), a multisubunit E3 ubiquitin ligase complex. The moderately UV sensitive Δrad4 strain became highly sensitive when cdc20-1 was present, and was rescued by overexpression of CDC20. The double mutant is also deficient in elicting RNR3-lacZ transcription upon exposure to UV irradiation or 4-NQO compared with the Δrad4 single mutant. We demonstrate that the Δrad4/cdc20-1 double mutant is defective in double strand break repair by way of a plasmid end-joining assay, indicating that Rad4 acts to ensure that damaged DNA is repaired via a Cdc20-mediated mechanism. This study is the first to present evidence that Cdc20 may play a role in the degradation of proteins involved in nucleotide excision repair.

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Roles for Gcn5p and Ada2p in transcription and nucleotide excision repair at the Saccharomyces cerevisiae MET16 gene

Nucleic Acids Research ◽

10.1093/nar/gkj501 ◽

2006 ◽

Vol 34 (3) ◽

pp. 976-985 ◽

Cited By ~ 15

Author(s):

J. A. Ferreiro

Keyword(s):

Saccharomyces Cerevisiae ◽

Nucleotide Excision Repair ◽

Excision Repair ◽

Nucleotide Excision

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Inhibition ofUVSSAubiquitination suppresses transcription‐coupled nucleotide excision repair deficiency caused by dissociation fromUSP7

FEBS Journal ◽

10.1111/febs.14382 ◽

2018 ◽

Vol 285 (5) ◽

pp. 965-976 ◽

Cited By ~ 6

Author(s):

Mitsuru Higa ◽

Kiyoji Tanaka ◽

Masafumi Saijo

Keyword(s):

Nucleotide Excision Repair ◽

Excision Repair ◽

Repair Deficiency ◽

Nucleotide Excision

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Nucleotide excision repair in the yeast Saccharomyces cerevisiae : its relationship to specialized mitotic recombination and RNA polymerase II basal transcription

Philosophical Transactions of the Royal Society B Biological Sciences ◽

10.1098/rstb.1995.0010 ◽

1995 ◽

Vol 347 (1319) ◽

pp. 63-68 ◽

Cited By ~ 27

Keyword(s):

Saccharomyces Cerevisiae ◽

Rna Polymerase ◽

Rna Polymerase Ii ◽

Nucleotide Excision Repair ◽

Excision Repair ◽

Gene Products ◽

Basal Transcription ◽

Yeast Saccharomyces Cerevisiae ◽

Nucleotide Excision ◽

Yeast Genes

Nucleotide excision repair (ner) in eukaryotes is a biochemically complex process involving multiple gene products. The budding yeast Saccharomyces cerevisiae is an informative model for this process. Multiple genes and in some cases gene products that are indispensable for ner have been isolated from this organism. Homologues of many of these yeast genes are structurally and functionally conserved in higher organisms, including humans. The yeast Rad1/Rad10 heterodimeric protein complex is an endonuclease that is believed to participate in damage-specific incision of DNA during ner . This endonuclease is also required for specialized types of recombination. The products of the RAD3, SSL2(RAD25) SSL1 and TFB1 genes have dual roles in ner and in RNA polymerase II-dependent basal transcription.

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