Modeling oncogenic translocations: Distinct roles for double-strand break repair pathways in translocation formation in mammalian cells

DNA Repair ◽  
2006 ◽  
Vol 5 (9-10) ◽  
pp. 1065-1074 ◽  
Author(s):  
David M. Weinstock ◽  
Christine A. Richardson ◽  
Beth Elliott ◽  
Maria Jasin
Genetics ◽  
2000 ◽  
Vol 154 (3) ◽  
pp. 1281-1289 ◽  
Author(s):  
Julang Li ◽  
Mark D Baker

Abstract We examined mechanisms of mammalian homologous recombination using a gene targeting assay in which the vector-borne region of homology to the chromosome bore small palindrome insertions that frequently escape mismatch repair when encompassed within heteroduplex DNA (hDNA). Our assay permitted the product(s) of each independent recombination event to be recovered for molecular analysis. The results revealed the following: (i) vector-borne double-strand break (DSB) processing usually did not yield a large double-strand gap (DSG); (ii) in 43% of the recombinants, the results were consistent with crossover at or near the DSB; and (iii) in the remaining recombinants, hDNA was an intermediate. The sectored (mixed) genotypes observed in 38% of the recombinants provided direct evidence for involvement of hDNA, while indirect evidence was obtained from the patterns of mismatch repair (MMR). Individual hDNA tracts were either long or short and asymmetric or symmetric on the one side of the DSB examined. Clonal analysis of the sectored recombinants revealed how vector-borne and chromosomal markers were linked in each strand of individual hDNA intermediates. As expected, vector-borne and chromosomal markers usually resided on opposite strands. However, in one recombinant, they were linked on the same strand. The results are discussed with particular reference to the double-strand-break repair (DSBR) model of recombination.


2010 ◽  
Vol 79 (2) ◽  
pp. 316-330 ◽  
Author(s):  
Richa Gupta ◽  
Daniel Barkan ◽  
Gil Redelman-Sidi ◽  
Stewart Shuman ◽  
Michael S. Glickman

Genetics ◽  
2004 ◽  
Vol 168 (3) ◽  
pp. 1539-1555 ◽  
Author(s):  
Erin C. Birmingham ◽  
Shauna A. Lee ◽  
Richard D. McCulloch ◽  
Mark D. Baker

2008 ◽  
Vol 29 (3) ◽  
pp. 519-527 ◽  
Author(s):  
Susanne Andrea Gatz ◽  
Marlen Keimling ◽  
Cindy Baumann ◽  
Thilo Dörk ◽  
Klaus-Michael Debatin ◽  
...  

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