scholarly journals Analysis of circulating microRNA during early gestation in Japanese Black cattle

2021 ◽  
pp. 106706
Author(s):  
K. Ono ◽  
S. Okamoto ◽  
C. Ninomiya ◽  
N. Toji ◽  
T. Kanazawa ◽  
...  
Keyword(s):  
Circulating Microrna ◽  
Japanese Black Cattle ◽  
Early Gestation

scholarly journals Analysis of Circulating microRNA During Early Gestation in Japanese Black Cattle

2021 ◽  
Author(s):  
Kasumi Ono ◽  
Sakura Okamoto ◽  
Chiaki Ninomiya ◽  
Noriyuki Toji ◽  
Tomomi Kanazawa ◽  
...  
Keyword(s):  
Estrous Cycle ◽  
Early Pregnancy ◽  
Circulating Microrna ◽  
Circulating Mirnas ◽  
Japanese Black Cattle ◽  
Circulating Micrornas ◽  
Physiological Conditions ◽  
Pregnancy Diagnosis ◽  
Bovine Plasma ◽  
Differentially Expressed Mirnas

Abstract Background Circulating microRNAs (miRNAs) are biomarkers for various diseases and physiological conditions in humans and mice; studies in domestic animals, particularly cattle, are limited. The importance of early pregnancy diagnosis (especially within the 21 day cow estrous cycle) in the livestock industry is extremely high. This study compared the circulating miRNAs in non-pregnant and pregnant Japanese Black cows, explored miRNAs as biomarkers for early pregnancy diagnosis, and established a measurement system that includes reference miRNA selection and the effect of hemolysis. Methods miRNA was extracted from the plasma of Japanese Black cows on day 21 after artificial insemination and subjected to a customized bovine oligonucleotide microarray for expression analysis. Differentially expressed miRNAs and reference miRNA candidates were selected and validated by real-time quantification PCR (RT-qPCR). Their stability was evaluated using NormFinder software. Hemolyzed samples were prepared using plasma from five cows in estrous cycle and subjected to RT-qPCR. Results A total of 124 miRNAs were detected in bovine plasma by microarray analysis in non-pregnant and pregnant cows. The levels of five circulating miRNAs were significantly higher in pregnant cows than in non-pregnant cows, and 24 miRNAs were detected only in the pregnant group. NormFinder analysis and RT-qPCR validation showed that miR-2455 was an appropriate reference miRNA in the plasma of non-pregnant and pregnant Japanese Black cows and miR-19b, miR-25, miR-29a, and miR-148a were significantly higher in the pregnant group. These four circulating miRNAs did not change during the estrous cycle and were less affected by hemolysis. Conclusions In the current study, we found high levels of four miRNAs in the plasma of pregnant Japanese Black cows. Since these miRNAs are less affected by hemolysis, they may potentially be used as markers for early pregnancy diagnosis in cattle.

Identification of circulating microrna pattern alteration during the tumor development using C38/C57BL/6 mouse tumor model

2012 ◽  
Vol 50 (05) ◽  
Author(s):  
Z Nagy ◽  
B Barták ◽  
S Spisák ◽  
B Wichmann ◽  
A Kalmár ◽  
...  
Keyword(s):  
Tumor Development ◽  
Tumor Model ◽  
Circulating Microrna ◽  
Mouse Tumor ◽  
Mouse Tumor Model

Systolic and diastolic heart function in early gestation between 5–10 weeks

2008 ◽  
Vol 29 (S 2) ◽  
Author(s):  
A Wloch ◽  
W Rozmus-Warcholinska ◽  
B Czuba ◽  
D Borowski ◽  
K Sodowski
Keyword(s):  
Heart Function ◽  
Early Gestation

Effects of dexamethasone and adrenocorticotropin on circulating microRNA expression in humans

2015 ◽  
Author(s):  
Ivan Igaz ◽  
Gabor Nyiro ◽  
Zoltan Nagy ◽  
Pal Perge ◽  
Miklos Toth ◽  
...  
Keyword(s):  
Microrna Expression ◽  
Circulating Microrna

Do early gestation maternal body composition parameters identify neonates born large for gestational age?

2020 ◽  
Author(s):  
Alexandra Cremona ◽  
Amanda Cotter ◽  
Khadijah Ismail ◽  
Kevin Hayes ◽  
Alan Donnelly ◽  
...  
Keyword(s):  
Body Composition ◽  
Gestational Age ◽  
Large For Gestational Age ◽  
Maternal Body ◽  
Early Gestation

162-LB: Impact of High-Fat Meal on Circulating microRNA in Youth with T1DM

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 162-LB
Author(s):  
JEANIE B. TRYGGESTAD ◽  
APRIL M. TEAGUE ◽  
KEVIN R. SHORT
Keyword(s):  
Circulating Microrna ◽  
High Fat ◽  
Fat Meal
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