Pharmacological characterization of urinary bladder smooth muscle contractility following partial bladder outlet obstruction in pigs

2006 ◽  
Vol 532 (1-2) ◽  
pp. 107-114 ◽  
Author(s):  
Ivan Milicic ◽  
Steven A. Buckner ◽  
Anthony Daza ◽  
Michael Coghlan ◽  
Thomas A. Fey ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Bladder Outlet Obstruction ◽  
Outlet Obstruction ◽  
Bladder Smooth Muscle ◽  
Muscle Contractility ◽  
Smooth Muscle Contractility ◽  
Pharmacological Characterization ◽  
Partial Bladder Outlet Obstruction ◽  
Urinary Bladder Smooth Muscle

Partial bladder outlet obstruction alters Ca2+ sensitivity of force, but not of MLC phosphorylation, in bladder smooth muscle

2003 ◽  
Vol 285 (4) ◽  
pp. F703-F710 ◽  
Author(s):  
Michaela C. Stanton ◽  
Michele Clement ◽  
Edward J. Macarak ◽  
Stephen A. Zderic ◽  
Robert S. Moreland
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Bladder Outlet Obstruction ◽  
Outlet Obstruction ◽  
Muscle Cells ◽  
Maximal Velocity ◽  
Bladder Smooth Muscle ◽  
Partial Bladder Outlet Obstruction ◽  
Velocity Of Shortening ◽  
Mlc Phosphorylation

Partial bladder outlet obstruction in the rabbit produces changes in bladder function similar to those seen clinically in patients with obstructive uropathies. Whole organ function is significantly altered, as are the smooth muscle cells inside the bladder wall. This study was designed to determine whether outlet obstruction alters smooth muscle function at the level of contractile filaments. Rabbit bladders were partially obstructed for 2 wk. Triton X-100 was used to provide a detergent-skinned bladder smooth muscle preparation that would allow control of the intracellular environment while the ability to shorten and develop force is maintained. Ca2+-force and Ca2+-myosin light chain (MLC) phosphorylation relations and maximal velocity of shortening were determined. The Ca2+ sensitivity of force was significantly lower in tissues from animals subjected to outlet obstruction compared with tissues from control animals. In contrast, no difference was noted in the Ca2+ sensitivity of MLC phosphorylation. Maximal levels of stress and MLC phosphorylation were similar in both animal groups. Maximal velocity of shortening was significantly slower in tissues from outlet-obstructed animals at all Ca2+ concentrations compared with tissues from control animals. Ultrastructurally, detergent skinning had little effect on structural integrity. Moreover, tissues from obstructed animals showed an increase in the number of sarcolemmal attachment plaque structures. We suggest that partial bladder outlet obstruction produces deleterious (e.g., decrease in Ca2+ sensitivity of force) and compensatory (e.g., increase in membrane attachment plaques) changes in bladder smooth muscle cells.

The Inhibitory Effect of the Flavonoid Galangin on Urinary Bladder Smooth Muscle Contractility is Mediated in Part by Modulation of Ca2+Release from Intracellular Stores

Planta Medica ◽  
2005 ◽  
Vol 71 (10) ◽  
pp. 962-964 ◽  
Author(s):  
Miriam Dambros ◽  
Marjanka van Deutekom ◽  
Rik de Jongh ◽  
Gommert A van Koeveringe ◽  
Jo G. De Mey ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Inhibitory Effect ◽  
Bladder Smooth Muscle ◽  
Muscle Contractility ◽  
Smooth Muscle Contractility ◽  
Urinary Bladder Smooth Muscle
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