Rat epidermal keratinocyte organotypic culture (ROC) compared to human cadaver skin: The effect of skin permeation enhancers

2007 ◽  
Vol 30 (3-4) ◽  
pp. 240-250 ◽  
Author(s):  
Sari Pappinen ◽  
Sanna Tikkinen ◽  
Sanna Pasonen-Seppänen ◽  
Lasse Murtomäki ◽  
Marjukka Suhonen ◽  
...  
Keyword(s):  
Organotypic Culture ◽  
Skin Permeation ◽  
Human Cadaver ◽  
Permeation Enhancers ◽  
Epidermal Keratinocyte ◽  
Human Cadaver Skin ◽  
Cadaver Skin

scholarly journals Nanoemulsions as vehicles for transdermal delivery of glycyrrhizin

2011 ◽  
Vol 47 (4) ◽  
pp. 769-778 ◽  
Author(s):  
Ranjit Kumar Harwansh ◽  
Kartik Chandra Patra ◽  
Surendra Kumar Pareta ◽  
Jagadish Singh ◽  
Mohammed Akhlaquer Rahman
Keyword(s):  
Transdermal Delivery ◽  
Isopropyl Alcohol ◽  
Skin Permeation ◽  
Skin Irritation ◽  
Human Cadaver ◽  
Permeation Enhancers ◽  
Brij 35 ◽  
Human Cadaver Skin ◽  
Cadaver Skin ◽  
Span 80

The present investigation aims to evaluate an isotropic and thermodynamically stable nanoemulsion formulation for transdermal delivery of glycyrrhizin (GZ), with minimum surfactant and cosurfactant (Smix) concentrations that could improve its solubility, permeation enhancement, and stability. Pseudo-ternary phase diagrams were developed and various nanoemulsion formulations were prepared using soyabean oil as oil, Span 80, Brij 35 as a surfactant and isopropyl alcohol as a cosurfactant. Nanoemulsion formulations that passed the thermodynamic stability tests were characterized for pH, viscosity and droplet size using a transmission electron microscopy. The transdermal ability of glycyrrhizin through human cadaver skin was determined using Franz diffusion cells. The in vitro skin permeation profile of the optimized nanoemulsion formulation (NE2) was compared to that of conventional gel. A significant increase in permeability parameters such as steady-state flux (Jss) and permeability coefficient (Kp) was observed in the optimized nanoemulsion formulation (NE2), which consisted of 1% wt/wt of mono ammonium glycyrrhizinate (MAG), 32.4% Span 80, 3.7% Brij 35, 10% isopropyl alcohol, 46.5% soyabean oil and 6.4% distilled water. No obvious skin irritation was observed for the studied nanoemulsion formulation (NE2) or the gel. The results indicated that nanoemulsions are promising vehicles for transdermal delivery of glycyrrhizin through human cadaver skin, without the use of additional permeation enhancers, because excipients of nanoemulsions act as permeation enhancers themselves.

scholarly journals Thymoquinone-Loaded Polymeric Films and Hydrogels for Bacterial Disinfection and Wound Healing

Biomedicines ◽  
2020 ◽  
Vol 8 (10) ◽  
pp. 386
Author(s):  
Anika Haq ◽  
Suneel Kumar ◽  
Yong Mao ◽  
Francois Berthiaume ◽  
Bozena Michniak-Kohn
Keyword(s):  
Staphylococcus Aureus ◽  
Wound Healing ◽  
Wound Infection ◽  
Skin Permeation ◽  
Control Group ◽  
Significant Activity ◽  
Human Cadaver ◽  
Human Cadaver Skin ◽  
Cadaver Skin

The purpose of this study was to synthesize and characterize novel biocompatible topical polymeric film and hydrogel systems that have the potential to deliver the antibacterial agent thymoquinone (TQ) directly to the skin target site to manage the local wound infection and thereby wound healing. The polyvinyl pyrrolidone (PVP) matrix-type films containing TQ were prepared by the solvent casting method. In vitro skin permeation studies on human cadaver skin produced a mean flux of 2.3 µg TQ/cm2/h. Human keratinocyte monolayers subjected to a scratch wound (an in vitro wound healing assay) showed 85% wound closure at day 6 in the TQ group (100 ng/mL TQ) as compared to 50% in the vehicle control group (p = 0.0001). In a zone-of-inhibition (ZOI) assay, TQ-containing films and hydrogels completely wiped out Staphylococcus aureus in 10 cm diameter Tryptic Soy Agar plates while 500 µg/mL gentamicin containing filters gave 10 mm of ZOI. In an ex vivo model, TQ-containing films eradicated bacterial colonization on human cadaver skin. Furthermore, in a full-thickness wound infection model in mice, TQ-containing films showed significant activity in controlling Staphylococcus aureus infection, thereby disinfecting the skin wound. In summary, TQ-containing PVP films and hydrogels developed in this study have the potential to treat and manage wound infections.

scholarly journals Alfuzosin hydrochloride transdermal films: evaluation of physicochemical, in vitro human cadaver skin permeation and thermodynamic parameters

2009 ◽  
Vol 35 (6) ◽  
pp. 716-729 ◽  
Author(s):  
Satyanarayan Pattnaik ◽  
Swain Kalpana ◽  
Parthagan Choudhury ◽  
Pradeepta K. Acharya ◽  
Subrata Mallick
Keyword(s):  
Thermodynamic Parameters ◽  
Skin Permeation ◽  
Human Cadaver ◽  
Human Cadaver Skin ◽  
Cadaver Skin ◽  
Alfuzosin Hydrochloride

scholarly journals Survival of human cadaver skin on severe combined immune deficiency pigs: Proof of concept

2019 ◽  
Vol 27 (4) ◽  
pp. 426-430 ◽  
Author(s):  
Adam J. Singer ◽  
Christopher Tuggle ◽  
Amanda Ahrens ◽  
Mary Sauer ◽  
Steve A. McClain ◽  
...  
Keyword(s):  
Immune Deficiency ◽  
Human Cadaver ◽  
Proof Of Concept ◽  
Severe Combined Immune Deficiency ◽  
Human Cadaver Skin ◽  
Cadaver Skin ◽  
Combined Immune Deficiency

Permeability and Retention Studies of (-)Epicatechin Gel Formulations in Human Cadaver Skin

2006 ◽  
Vol 32 (9) ◽  
pp. 1007-1017 ◽  
Author(s):  
J. Suppasrivasuseth ◽  
R. A. Bellantone ◽  
F. M. Plakogiannis ◽  
G. Stagni
Keyword(s):  
Human Cadaver ◽  
Human Cadaver Skin ◽  
Cadaver Skin ◽  
Gel Formulations

Morphology of glycerol-preserved human cadaver skin

Burns ◽  
1996 ◽  
Vol 22 (2) ◽  
pp. 113-116 ◽  
Author(s):  
C.D. Richters ◽  
M.J. Hoekstra ◽  
J. van Baare ◽  
J.S. du Pont ◽  
E.W.A. Kamperdijk
Keyword(s):  
Human Cadaver ◽  
Human Cadaver Skin ◽  
Cadaver Skin

Permeability of pure enantiomers of ketorolac through human cadaver skin

1995 ◽  
Vol 84 (8) ◽  
pp. 987-990 ◽  
Author(s):  
Samir D. Roy ◽  
Elizabeth Manoukian ◽  
Alan Divor ◽  
Dhruba J. Chatterjee
Keyword(s):  
Human Cadaver ◽  
Human Cadaver Skin ◽  
Cadaver Skin
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