Effect of EDTA and EDDS on phytoremediation of Pb- and Zn- contaminated soil by Brassica Juncea was investigated in this work. Especially, the effect of the kind and the method of adding chelating agent was investigated during the plant growth. Plants were grown in an environmental control system. The biomass of the whole plant was weighed, and the uptake of Pb and Zn in shoot and root were determined using ICP-AES. Consequently, the following matters have been obtained: (1) Both EDTA and EDDS significantly enhanced the translocation of metals (Pb and Zn) in soil from root to shoot. Furthermore, the two chelating agents resulted in a sharply biomass loss for more than 30% of the control. As a result, the total uptake amount of metals by Brassica Juncea was decreased (except the uptake of Pb with the addition of 3.0 mmol•kg-1 EDTA). (2) EDDS showed the higher inhibition for the growth of Brassica Juncea than EDTA. (3) The method for adding EDTA and EDDS at several times separately did not necessarily increase the uptake of heavy metals.
Regulation of Root-zone Temperature by Root-zone Environmental Control System affects Growth and Flowering of Verbena ^|^times; hybrida and Pelargonium ^|^times; hortorum
