Plasmodium falciparum: Differing effects of non-esterified fatty acids and phospholipids on intraerythrocytic growth in serum-free medium

2011 ◽  
Vol 127 (3) ◽  
pp. 708-713 ◽  
Author(s):  
Hiroko Asahi ◽  
Shinji Izumiyama ◽  
Mohammed Essa Marghany Tolba ◽  
Bethel Kwansa-Bentum
Keyword(s):  
Fatty Acids ◽  
Plasmodium Falciparum ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Esterified Fatty Acids

Determination of Fifty Percent Inhibitory Concentrations (IC50) of Antimalarial Drugs against Plasmodium Falciparum Parasites in a Serum-Free Medium

1994 ◽  
Vol 51 (2) ◽  
pp. 214-218 ◽  
Author(s):  
Ayub O. Ofulla ◽  
Gladys M. Aleman ◽  
Aloys S. Orago ◽  
John I. Githure ◽  
Anthony J. Johnson ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Antimalarial Drugs ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

Immunotoxicity of Polyunsaturated Fatty Acids in Serum-Free Medium

1995 ◽  
Vol 17 (2) ◽  
pp. 365-383 ◽  
Author(s):  
B. U. Tezabwala ◽  
M. Bennett ◽  
S. M. Grundy
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

Continuous cultivation of intraerythrocytic Plasmodium falciparum in a serum-free medium with the use of a growth-promoting factor

Parasitology ◽  
1994 ◽  
Vol 109 (4) ◽  
pp. 397-401 ◽  
Author(s):  
H. Asahi ◽  
T. Kanazawa
Keyword(s):  
Plasmodium Falciparum ◽  
Bovine Serum ◽  
Basal Medium ◽  
Parasite Growth ◽  
Serum Free Medium ◽  
Free Medium ◽  
Commercial Preparation ◽  
Serum Free ◽  
Adult Bovine Serum ◽  
Growth Promoting

SUMMARYSerum-free media were used to culture Plasmodium falciparum. A commercial preparation, Daigo's GF21 developed as a growth-promoting factor for many kinds of mammalian cells, and consisting of the 55–70% ammonium sulphate fraction of adult bovine serum, insulin, transferrin, ethanolamine and sodium selenite, was found to sustain growth of the parasite when Daigo's T was employed as a basal medium. The optimal Daigo's GF21 concentration for parasite growth was between 5 and 20% (v/v), with the best results at 10%. Differential counts indicated that Daigo's GF21 is essential for schizogony. Established serum-free medium, GIT, consisting of Daigo's T basal medium and Daigo's GF21, yielded good parasite growth without any supplementation. Growth-promoting factor derived from adult bovine serum in Daigo's GF21 was shown to be crucial to parasite growth. The results presented here will not only be of practical value, but will provide important information about the developmental requirements for the parasite.

Cultivation of Plasmodium falciparum Parasites in a Serum-Free Medium

1993 ◽  
Vol 49 (3) ◽  
pp. 335-340 ◽  
Author(s):  
Ayub V. O. Ofulla ◽  
Baldip Khan ◽  
Anthony J. Johnson ◽  
John I. Githure ◽  
Samuel K. Martin ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

Oleic acid is indispensable for intraerythrocytic proliferation of Plasmodium falciparum

Parasitology ◽  
2007 ◽  
Vol 134 (12) ◽  
pp. 1671-1677 ◽  
Author(s):  
F. MI-ICHI ◽  
S. KANO ◽  
T. MITAMURA
Keyword(s):  
Fatty Acids ◽  
Plasmodium Falciparum ◽  
Oleic Acid ◽  
Palmitic Acid ◽  
Vaccenic Acid ◽  
Parasite Development ◽  
Serum Free Medium ◽  
Free Medium ◽  
Erythrocytic Cycle

SUMMARYSerum-derived fatty acids are essential for the intraerythrocytic proliferation of Plasmodium falciparum in humans. We previously reported that only limited combinations of fatty acids can support long-term parasite culture, and palmitic acid (C16:0)/oleic acid (C18:1, n-9), palmitic acid (C16:0)/vaccenic acid (C18:1, n-7), or stearic acid (C18:0) are required in these combinations, implying that these fatty acids are key molecules for intraerythrocytic parasite growth (Mi-Ichi et al.2006). Here, we analysed profiles of parasitaemia changes as well as morphologies during the erythrocytic cycle and confirmed the importance of C16:0 and C18:1, n-9. We also provide evidence that C18:1, n-9 but not other C18 monoenoic or dienoic acids maintain the synchronicity of parasite development in serum-free medium when paired with C16:0, resulting in maintained exponential growth. Thus, C18:1, n-9 is indispensable for the intraerythrocytic proliferation of P. falciparum.

Exogeneous factors are not necessary in attachment, spreading and polarization of hela cells

1978 ◽  
Vol 36 (2) ◽  
pp. 310-311
Author(s):  
W. Liebrich
Keyword(s):  
Hela Cells ◽  
Calf Serum ◽  
Glass Tube ◽  
Serum Free Medium ◽  
Nacl Solution ◽  
Free Medium ◽  
Minimum Essential Medium ◽  
Serum Free ◽  
All Solutions ◽  
Glass Support

HeLa cells were grown for 2-3 days in EAGLE'S minimum essential medium with 10% calf serum (S-MEM; Seromed, München) and then incubated for 24 hours in serum free medium (MEM). After detaching the cells with a solution of 0. 14 % EDTA and 0. 07 % trypsin (Difco, 1 : 250) they were suspended in various solutions (S-MEM = control, MEM, buffered salt solutions with or without Me++ions, 0. 9 % NaCl solution) and allowed to settle on glass tube slips (Leighton-tubes). After 5, 10, 15, 20, 25, 30, 1 45, 60 minutes 2, 3, 4, 5 hours cells were prepared for scanning electron microscopy as described by Paweletz and Schroeter. The preparations were examined in a Jeol SEM (JSM-U3) at 25 KV without tilting.The suspended spherical HeLa cells are able to adhere to the glass support in all solutions. The rate of attachment, however, is faster in solutions without serum than in the control. The latter is in agreement with the findings of other authors.

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