Photomorphogenesis in the hypogeous fungus Tuber borchii: isolation and characterization of Tbwc-1, the homologue of the blue-light photoreceptor of Neurospora crassa

2004 ◽  
Vol 41 (7) ◽  
pp. 688-697 ◽  
Author(s):  
R. Ambra ◽  
B. Grimaldi ◽  
S. Zamboni ◽  
P. Filetici ◽  
G. Macino ◽  
...  
Keyword(s):  
Neurospora Crassa ◽  
Blue Light ◽  
Tuber Borchii ◽  
Isolation And Characterization ◽  
Blue Light Photoreceptor ◽  
Hypogeous Fungus

scholarly journals ISOLATION AND CHARACTERIZATION OF LIGHT-INSENSITIVE MUTANTS OF NEUROSPORA CRASSA

Genetics ◽  
1983 ◽  
Vol 104 (1) ◽  
pp. 11-21
Author(s):  
John Paietta ◽  
Malcolm L Sargent
Keyword(s):  
Genetic Analysis ◽  
Neurospora Crassa ◽  
Blue Light ◽  
Light Sensitivity ◽  
Phase Shifting ◽  
Nuclear Genes ◽  
Wild Type ◽  
Linkage Groups ◽  
Isolation And Characterization

ABSTRACT As part of a genetic analysis of blue light photoreception in Neurospora, three mutants were isolated that do not exhibit photosuppression of circadian conidiation, i.e., they show periodic conidiation in constant light. The mutations have been given the designations lis-1, lis-2 and lis-3 ("light insensitive"). The three mutations segregate as single nuclear genes, are nonallelic and are recessive to wild type in heterokaryon tests. The linkage groups of the mutations are as follows: lis-1, I; lis-2, VI; and lis-3, V. The light -insensitive phenotype of the mutants is restricted to the photosuppression response; other responses such as photoinduced phase shifting of the conidiation rhythm and photoinduced carotenogenesis are not altered. The physiological or biochemical defects of the mutants have not been established, but they are not similar to previous reported cases (i.e., rib and poky) in which a reduction in light sensitivity has been observed.

scholarly journals ISOLATION AND CHARACTERIZATION OF NUCLEASE MUTANTS IN NEUROSPORA CRASSA

Genetics ◽  
1969 ◽  
Vol 63 (1) ◽  
pp. 75-92
Author(s):  
T Ishikawa ◽  
A Toh-E ◽  
I Uno ◽  
K Hasunuma
Keyword(s):  
Neurospora Crassa ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of a Mitochondrially Synthesized Polypeptide from Neurospora crassa cni-1 Mutant

1975 ◽  
Vol 356 (2) ◽  
pp. 1595-1604 ◽  
Author(s):  
Rainer Michel ◽  
Anton Liebl ◽  
Werner Machleidt ◽  
Joachim Otto ◽  
Walter Neupert
Keyword(s):  
Neurospora Crassa ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Differentially Expressed Genes in the Mycelium and Fruit Body of Tuber borchii

2002 ◽  
Vol 68 (11) ◽  
pp. 5788-5788 ◽  
Author(s):  
Isabelle Lacourt ◽  
Sébastien Duplessis ◽  
Simona Abbà ◽  
Paola Bonfante ◽  
Francis Martin
Keyword(s):  
Differentially Expressed Genes ◽  
Fruit Body ◽  
Differentially Expressed ◽  
Tuber Borchii ◽  
Isolation And Characterization

scholarly journals ISOLATION AND CHARACTERIZATION OF CHROMATIN FROM NEUROSPORA CRASSA

1969 ◽  
Vol 43 (1) ◽  
pp. 51-58 ◽  
Author(s):  
R. S. Dwivedi ◽  
S. K. Dutta ◽  
D. P. Bloch
Keyword(s):  
Amino Acid ◽  
Neurospora Crassa ◽  
Polyacrylamide Gel Electrophoresis ◽  
Average Diameter ◽  
Uv Absorption ◽  
Eosin Y ◽  
Basic Proteins ◽  
Isolation And Characterization ◽  
Calf Thymus

Different preparations of chromatin isolated from mycelia of Neurospora crassa were analyzed for DNA-associated RNA and proteins. The UV absorption spectra, the ultrastructure of chromatin, and the amino acid composition of the acid-extractable proteins were studied. The protein:DNA ratios range from 1.5 to 2.8; the RNA:DNA ratios range from 0.5 to 1.24. UV absorption shows a macimum at 259 mµ and a minimum at 238–239 mµ. The E280/E260 ranges from 0.59 to 0.70. Electron microscopy reveals a fibrous structure with individual fibers of 120–150 A average diameter. Attempts were made to study the protein by polyacrylamide gel electrophoresis and amino acid analysis. The results indicate that Neurospora chromatin does not contain basic proteins comparable to calf thymus histone. The ratios of basic to acidic amino acids range from 0.93 to 1.19. On electrophoresis, no bands are seen whose positions correspond to those of histones. Staining for basic proteins with fast green or eosin Y at pH 8.2 also shows a negative reaction, suggesting the absence of histones.

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