scholarly journals Dabsyl derivatisation as an alternative for dansylation in the detection of biogenic amines in fermented meat products by reversed phase high performance liquid chromatography

2012 ◽  
Vol 130 (4) ◽  
pp. 1017-1023 ◽  
Author(s):  
E. De Mey ◽  
G. Drabik-Markiewicz ◽  
H. De Maere ◽  
M.-C. Peeters ◽  
G. Derdelinckx ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Biogenic Amines ◽  
High Performance ◽  
Meat Products ◽  
Reversed Phase

scholarly journals Detection and quantitation of additions of soybean proteins in cured-meat products by perfusion reversed-phase high-performance liquid chromatography

2005 ◽  
Vol 28 (9-10) ◽  
pp. 987-995 ◽  
Author(s):  
Mónica Criado ◽  
Florentina Castro-Rubio ◽  
Carmen García-Ruiz ◽  
María Concepción García ◽  
María Luisa Marina
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Meat Products ◽  
Reversed Phase ◽  
Soybean Proteins ◽  
Cured Meat

N-acetylation of biogenic amines in Ascaridia galli

Parasitology ◽  
1991 ◽  
Vol 102 (3) ◽  
pp. 445-450 ◽  
Author(s):  
R. E. Isaac ◽  
L. Eaves ◽  
R. Muimo ◽  
N. Lamango
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Biogenic Amines ◽  
High Performance ◽  
Reversed Phase ◽  
Supernatant Fraction ◽  
Ascaridia Galli ◽  
Acetyl Coa ◽  
Experimental Conditions ◽  
Enzyme Preparations

SUMMARYThe metabolism of 5-hydroxytryptamine (5-HT), octopamine (OA) and dopamine (DA) by adult male Ascaridia galli was investigated by incubating worms cut into 5 mm lengths with radio-isotope labelled amine. [14C]5-hydroxytryptamine and [14C]octopamine were taken up by the tissues and were metabolized to apolar products that co-chromatographed using both high-performance liquid chromatography and thin-layer chromatography with N-acetyl 5-hydroxytryptamine (NA5-HT) and N-acetyloctopamine (NAOA), respectively. N-acetylation was by far the most important reaction detected under these experimental conditions. A brief incubation of cut worms with [3H]dopamine resulted in the formation of a radio-isotope labelled metabolite that co-chromatographed with N-acetyldopamine (NADA) on reversed-phase high-performance liquid chromatography. The N-acetyltransferase activity towards 5-hydroxytryptamine and octopamine was detected in crude homogenates of male worms only when the co-substrate, acetyl CoA, was added to the reaction mixture. This enzyme activity appeared to be mainly localized in a 40 000 g supernatant fraction. The failure of previous studies to detect N-acetylation of biogenic amines in tissue homogenates of nematodes may have been due to the low levels of acetyl CoA present in these enzyme preparations.

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