Rapid purification of lysozyme by mixed-mode adsorption chromatography in stirred fluidized bed

2019 ◽  
Vol 272 ◽  
pp. 619-627 ◽  
Author(s):  
Kuei-Hsiang Chen ◽  
Shin-Ying Chou ◽  
Yu-Kaung Chang
Keyword(s):  
Fluidized Bed ◽  
Mixed Mode ◽  
Adsorption Chromatography ◽  
Rapid Purification

RAPID PURIFICATION OF TRI-IODOTHYRONINE AND THYROXINE PROTEIN CONJUGATES FOR ANTIBODY PRODUCTION

1975 ◽  
Vol 65 (1) ◽  
pp. 133-138 ◽  
Author(s):  
C. W. BURKE ◽  
R. A. SHAKESPEAR
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Antibody Production ◽  
Guinea Pigs ◽  
High Titre ◽  
Adsorption Chromatography ◽  
Protein Conjugates ◽  
Rapid Purification ◽  
Dialysis Technique

SUMMARY Thyroxine (T4) and tri-iodothyronine (T3) were coupled to human serum albumin (HSA) with carbodi-imide. By adsorption chromatography on Sephadex G-25, fractions containing purified conjugate, but not reversibly-bound T3 or T4, were obtained, and this procedure took 5 h; considerably less than the conventional dialysis technique. Highly specific high-titre antisera were produced in rabbits and guinea-pigs by injection of these fractions in Freund's adjuvant.

Uultrastructure of Microtubules

1972 ◽  
Vol 30 ◽  
pp. 252-253
Author(s):  
Ariaki Nagayama
Keyword(s):  
Fine Structure ◽  
Cultured Cells ◽  
Present Report ◽  
Confluent Monolayer ◽  
Purification Method ◽  
Vinca Rosea ◽  
L Cells ◽  
Antimitotic Activity ◽  
Monolayer Cultures ◽  
Rapid Purification

Vinblastine(Vb) or vincristine, alkaloid derived from Vinca rosea is known for its antimitotic activity by regrouping of microtubules into paracrystalline form within the cells. A rapid purification method of vinblastine-induced microtubular paracrystals(PC) has provided us with a fresh and pure microtubular material demonstrating the presence of a labile ATPase associated with the PC. The present report is concerned with the fine structure of purified microtubules of mammalian cultured cells.Confluent monolayer cultures of L cells were incubated for 20hrs with 10-5 M Vb (donated from Shionogi Seiyaku & Co., Osaka, Japan).

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