Two hypo-allergenic derivatives lacking the dominant linear epitope of Scy p 1 and Scy p 3

2021 ◽  
pp. 131588
Author(s):  
Mengsi Li ◽  
Fei Xia ◽  
Yiyu Chen ◽  
Meng Liu ◽  
Qingmei Liu ◽  
...  
Keyword(s):  
Linear Epitope

Recombinant PSP94 (prostate secretory protein of 94 amino acids) demonstrates similar linear epitope structure as natural PSP94 protein

1996 ◽  
Vol 63 (1) ◽  
pp. 61-73 ◽  
Author(s):  
Jim W. Xuan ◽  
Dongmei Wu ◽  
Yuzhen Guo ◽  
Jennifer E. Fraser ◽  
Joseph L. Chin
Keyword(s):  
Amino Acids ◽  
Secretory Protein ◽  
Linear Epitope ◽  
Epitope Structure

Linear epitope mapping by native mass spectrometry

2009 ◽  
Vol 395 (1) ◽  
pp. 100-107 ◽  
Author(s):  
Xiaojun Lu ◽  
Michael R. DeFelippis ◽  
Lihua Huang
Keyword(s):  
Mass Spectrometry ◽  
Epitope Mapping ◽  
Native Mass Spectrometry ◽  
Linear Epitope

Identification of a novel linear epitope in tetanus toxin recognized by a protective monoclonal antibody: Implications for vaccine design

Vaccine ◽  
2012 ◽  
Vol 30 (45) ◽  
pp. 6449-6455 ◽  
Author(s):  
Ping Luo ◽  
Liyan Qin ◽  
Xuhu Mao ◽  
Li Chen ◽  
Shu Yu ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Tetanus Toxin ◽  
Vaccine Design ◽  
Linear Epitope

scholarly journals (SWR x SJL)F1 mice: a new model of lupus-like disease.

1994 ◽  
Vol 179 (5) ◽  
pp. 1429-1435 ◽  
Author(s):  
S Vidal ◽  
C Gelpí ◽  
J L Rodríguez-Sánchez
Keyword(s):  
Lupus Erythematosus ◽  
Cell Number ◽  
Autoimmune Response ◽  
Linear Epitope ◽  
Igg Antibodies ◽  
Human Sera ◽  
Genetic Mechanisms ◽  
Dsdna Antibodies ◽  
Novel Model ◽  
Small Nuclear Ribonucleoproteins

During the study of autoimmune models we found that (SWR x SJL)F1 mice (both parental strains with the V beta a phenotype) spontaneously produced immunoglobulin G (IgG) antibodies directed against Sm/U1 small nuclear ribonucleoproteins (snRNPs). In some of these females, the presence of these autoantibodies was found as early as 10 wk of age. Their frequency increased with age i.e., 70% at 40 wk. At that time, only 10% of males developed anti-Sm/U1snRNP antibodies. Anti-Sm/U1snRNP antibodies from positive mice generally recognized the peptides BB', D, 70 kD, and A from RNPs. These polypeptides are known to bear the autoantigenic epitopes that are recognized by human sera containing anti-Sm and anti-U1snRNP antibodies. Reactivity of IgG antibodies with the octapeptide sequence PPPGMRPP was also found in 30% of anti-Sm/U1snRNP positive (SWR x SJL)F1 mice that precipitated BB' peptides. This octapeptide has been described as the most immunoreactive linear epitope in systemic lupus erythematosus (SLE) patients with anti-Sm and anti-U1snRNP antibodies. Approximately 30% of anti-Sn/U1snRNP positive females, later produced anti-dsDNA antibodies. This fact was accompanied by the development of proteinuria due to glomerulonephritis mediated by immunocomplexes. In addition to the specific autoimmune response, (SWR x SJL)F1 females also showed other immunologic abnormalities such as hypergammaglobulinemia, and an approximately twofold increase in spleen cell number compared with control mice. These results indicate that (SWR x SJL)F1 females develop clinical and serological abnormalities similar to those observed in human SLE and constitute a novel model for the study of the genetic mechanisms that result in autoimmunity.

scholarly journals Identification of a Linear Epitope on the Fusion Glycoprotein of Respiratory Syncytial Virus

1990 ◽  
Vol 71 (1) ◽  
pp. 53-59 ◽  
Author(s):  
G. E. Scopes ◽  
P. J. Watt ◽  
P. R. Lambden
Keyword(s):  
Respiratory Syncytial Virus ◽  
Linear Epitope ◽  
Fusion Glycoprotein ◽  
Syncytial Virus

scholarly journals A Novel Humanized Antibody Neutralizes H5N1 Influenza Virus via Two Different Mechanisms

2015 ◽  
Vol 89 (7) ◽  
pp. 3712-3722 ◽  
Author(s):  
Yunrui Tan ◽  
Qingyong Ng ◽  
Qiang Jia ◽  
Jimmy Kwang ◽  
Fang He
Keyword(s):  
Influenza Virus ◽  
Highly Pathogenic Avian Influenza ◽  
Conformational Epitope ◽  
Virus Neutralization ◽  
Linear Epitope ◽  
Highly Pathogenic ◽  
Pathogenic Avian Influenza ◽  
Pathogenic Avian Influenza Virus ◽  
Avian Influenza Virus Subtype ◽  
Virus Subtype

ABSTRACTHighly pathogenic avian influenza virus subtype H5N1 continues to be a severe threat to public health, as well as the poultry industry, because of its high lethality and antigenic drift rate. Neutralizing monoclonal antibodies (MAbs) can serve as a useful tool for preventing, treating, and detecting H5N1. In the present study, humanized H5 antibody 8A8 was developed from a murine H5 MAb. Both the humanized and mouse MAbs presented positive activity in hemagglutination inhibition (HI), virus neutralization, and immunofluorescence assays against a wide range of H5N1 strains. Interestingly, both human and murine 8A8 antibodies were able to detect H5 in Western blot assays under reducing conditions. Further, by sequencing of escape mutants, the conformational epitope of 8A8 was found to be located within the receptor binding domain (RBD) of H5. The linear epitope of 8A8 was identified by Western blotting of overlapping fragments and substitution mutant forms of HA1. Reverse genetic H5N1 strains with individual mutations in either the conformational or the linear epitope were generated and characterized in a series of assays, including HI, postattachment, and cell-cell fusion inhibition assays. The results indicate that for 8A8, virus neutralization mediated by RBD blocking relies on the conformational epitope while binding to the linear epitope contributes to the neutralization by inhibiting membrane fusion. Taken together, the results of this study show that a novel humanized H5 MAb binds to two types of epitopes on HA, leading to virus neutralization via two mechanisms.IMPORTANCERecurrence of the highly pathogenic avian influenza virus subtype H5N1 in humans and poultry continues to be a serious public health concern. Preventive and therapeutic measures against influenza A viruses have received much interest in the context of global efforts to combat the current and future pandemics. Passive immune therapy is considered to be the most effective and economically prudent preventive strategy against influenza virus besides vaccination. It is important to develop a humanized neutralizing monoclonal antibody (MAb) against all of the clades of H5N1. For the first time, we report in this study that a novel humanized H5 MAb binds to two types of epitopes on HA, leading to virus neutralization via two mechanisms. These findings further deepen our understanding of influenza virus neutralization.

scholarly journals Post-translational modifications of the major linear epitope 169?190aa of Ro60�kDa autoantigen alter the autoantibody binding

2006 ◽  
Vol 146 (1) ◽  
pp. 60-65 ◽  
Author(s):  
A. G. Terzoglou ◽  
J. G. Routsias ◽  
H. M. Moutsopoulos ◽  
A. G. Tzioufas
Keyword(s):  
Linear Epitope ◽  
Post Translational Modifications

Linear Epitope Mapping by the PEPSCAN Method

HIV Protocols ◽  
2003 ◽  
pp. 293-308
Author(s):  
Lawrence D. Loomis-Price
Keyword(s):  
Epitope Mapping ◽  
Linear Epitope

scholarly journals Virus-Like Particle Based Vaccines Elicit Neutralizing Antibodies against the HIV-1 Fusion Peptide

Vaccines ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 765
Author(s):  
Alemu Tekewe Mogus ◽  
Lihong Liu ◽  
Manxue Jia ◽  
Diane T. Ajayi ◽  
Kai Xu ◽  
...  
Keyword(s):  
Neutralizing Antibodies ◽  
Vaccination Strategy ◽  
Fusion Peptide ◽  
Linear Epitope ◽  
Broadly Neutralizing Antibodies ◽  
Virus Like Particle ◽  
Boost Immunization ◽  
Potential Vaccine ◽  
Hiv Envelope ◽  
Hiv 1

Broadly neutralizing antibodies (bnAbs) isolated from HIV-infected individuals delineate vulnerable sites on the HIV envelope glycoprotein that are potential vaccine targets. A linear epitope within the N-terminal region of the HIV-1 fusion peptide (FP8) is the primary target of VRC34.01, a bnAb that neutralizes ~50% of primary HIV isolates. FP8 has attracted attention as a potential HIV vaccine target because it is a simple linear epitope. Here, platform technologies based on RNA bacteriophage virus-like particles (VLPs) were used to develop multivalent vaccines targeting the FP8 epitope. Both recombinant MS2 VLPs displaying the FP8 peptide and Qβ VLPs displaying chemically conjugated FP8 peptide induced high titers of FP8-specific antibodies in mice. Moreover, a heterologous prime-boost-boost regimen employing the two FP8-VLP vaccines and native envelope trimer was the most effective approach for eliciting HIV-1 neutralizing antibodies. Given the potent immunogenicity of VLP-based vaccines, this vaccination strategy—inspired by bnAb-guided epitope mapping, VLP bioengineering, and prime-boost immunization approaches—may be a useful strategy for eliciting bnAb responses against HIV.

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