Construction of an impedimetric immunosensor for label-free detecting carbofuran residual in agricultural and environmental samples

Food Control ◽  
2015 ◽  
Vol 53 ◽  
pp. 72-80 ◽  
Author(s):  
Lin Liu ◽  
Dan Xu ◽  
Yinyue Hu ◽  
Shuzhao Liu ◽  
Honglin Wei ◽  
...  
Keyword(s):  
Environmental Samples ◽  
Label Free ◽  
Impedimetric Immunosensor

Label free Impedimetric Immunosensor for effective bladder Cancer detection in clinical urine samples

2020 ◽  
Vol 22 (3) ◽  
Author(s):  
Muhammad Omar Shaikh ◽  
Ting-Chi Huang ◽  
Ting-Feng Wu ◽  
Cheng-Hsin Chuang
Keyword(s):  
Bladder Cancer ◽  
Cancer Detection ◽  
Urine Samples ◽  
Label Free ◽  
Impedimetric Immunosensor

A Simple, Highly Sensitive, and Label-Free Impedimetric Immunosensor for Detection of Microcystin-LR in Water

2010 ◽  
Vol 43 (4) ◽  
pp. 533-544 ◽  
Author(s):  
Xiulan Sun ◽  
Hui Shi ◽  
Hongxin Wang ◽  
Liwen Xiao ◽  
Luning Li
Keyword(s):  
Label Free ◽  
Highly Sensitive ◽  
Impedimetric Immunosensor

Ultra trace analysis of small molecule by label-free impedimetric immunosensor using multilayer modified electrode

2011 ◽  
Vol 26 (11) ◽  
pp. 4571-4578 ◽  
Author(s):  
Kochaporn Chullasat ◽  
Proespichaya Kanatharana ◽  
Warakorn Limbut ◽  
Apon Numnuam ◽  
Panote Thavarungkul
Keyword(s):  
Modified Electrode ◽  
Small Molecule ◽  
Trace Analysis ◽  
Label Free ◽  
Ultra Trace ◽  
Impedimetric Immunosensor ◽  
Ultra Trace Analysis

scholarly journals Label-Free Detection of Chondroitin Sulphate Proteoglycan 4 by a Polyaniline/Graphene Nanocomposite Functionalized Impedimetric Immunosensor

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
JingJing Fu ◽  
ZhuanZhuan Shi ◽  
Man Li ◽  
Yangyang Wang ◽  
Ling Yu
Keyword(s):  
Cell Lines ◽  
Chondroitin Sulphate ◽  
Low Cost ◽  
Enzyme Linked Immunosorbent Assay ◽  
Label Free ◽  
Sulphate Proteoglycan ◽  
Chondroitin Sulphate Proteoglycan ◽  
Label Free Detection ◽  
Proteoglycan 4 ◽  
Impedimetric Immunosensor

The chondroitin sulphate proteoglycan 4 (CSPG4), also known as high molecular weight-melanoma associated antigen (HMW-MAA), is a tumor-associated antigen that is expressed in more than 85% of surgically removed melanoma lesions but has restricted distribution in normal tissues. The diagnostic and therapeutic value of CSPG4 drives a need for sensitive and low-cost detection approaches. To this end, we developed a polyaniline/graphene oxide nanocomposite (PANI@GO) that was electrochemically codeposited on indium tin oxide (ITO) electrode. Glutaraldehyde mediated the covalent immobilization of CSPG4 specific antibody mAbD2.8.5 to construct a CSPG4 immunosensor using cell culture media and cell lysate as samples. The fully assembled impedimetric immunosensor was used to detect CSPG4 in CSPG4-positive cell lines M14/CSPG4 and MV3. No impedance signal changes could be observed from CSPG4-negative cell lines M14 and mAbMk2-23 showing the specificity of the CSPG4-impedimetric immunosensor. This low-cost, simple, and label-free analytical method is an alternative to enzyme-linked immunosorbent assay and flow cytometry in screening of CSPG4 in complex biological samples.

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