An electrochemical impedimetric immunosensor for label-free detection of Campylobacter jejuni in diarrhea patients’ stool based on O-carboxymethylchitosan surface modified Fe3O4 nanoparticles

The chondroitin sulphate proteoglycan 4 (CSPG4), also known as high molecular weight-melanoma associated antigen (HMW-MAA), is a tumor-associated antigen that is expressed in more than 85% of surgically removed melanoma lesions but has restricted distribution in normal tissues. The diagnostic and therapeutic value of CSPG4 drives a need for sensitive and low-cost detection approaches. To this end, we developed a polyaniline/graphene oxide nanocomposite (PANI@GO) that was electrochemically codeposited on indium tin oxide (ITO) electrode. Glutaraldehyde mediated the covalent immobilization of CSPG4 specific antibody mAbD2.8.5 to construct a CSPG4 immunosensor using cell culture media and cell lysate as samples. The fully assembled impedimetric immunosensor was used to detect CSPG4 in CSPG4-positive cell lines M14/CSPG4 and MV3. No impedance signal changes could be observed from CSPG4-negative cell lines M14 and mAbMk2-23 showing the specificity of the CSPG4-impedimetric immunosensor. This low-cost, simple, and label-free analytical method is an alternative to enzyme-linked immunosorbent assay and flow cytometry in screening of CSPG4 in complex biological samples.

Download Full-text

Impedimetric immunosensor based on SWCNT-COOH modified gold microelectrodes for label-free detection of deep venous thrombosis biomarker

Biosensors and Bioelectronics ◽

10.1016/j.bios.2010.07.004 ◽

2010 ◽

Vol 26 (4) ◽

pp. 1278-1282 ◽

Cited By ~ 32

Author(s):

S. Bourigua ◽

M. Hnaien ◽

F. Bessueille ◽

F. Lagarde ◽

S. Dzyadevych ◽

...

Keyword(s):

Venous Thrombosis ◽

Deep Venous Thrombosis ◽

Label Free ◽

Label Free Detection ◽

Impedimetric Immunosensor

Download Full-text

Impedimetric immunosensor for the specific label free detection of ciprofloxacin antibiotic

Biosensors and Bioelectronics ◽

10.1016/j.bios.2007.07.014 ◽

2007 ◽

Vol 23 (4) ◽

pp. 549-555 ◽

Cited By ~ 53

Author(s):

Rodica E. Ionescu ◽

Nicole Jaffrezic-Renault ◽

Laurent Bouffier ◽

Chantal Gondran ◽

Serge Cosnier ◽

...

Keyword(s):

Label Free ◽

Label Free Detection ◽

Impedimetric Immunosensor ◽

Specific Label

Download Full-text

P1BS.7 - An impedimetric immunosensor based on magnetic graphene nanoribbons modified by boronic acid for sensitive label-free detection of lymphoma cancer cells

10.5162/imcs2018/p1bs.7 ◽

2018 ◽

Author(s):

P. Hashemi ◽

A. Afkhami ◽

H. Bagheri

Keyword(s):

Cancer Cells ◽

Boronic Acid ◽

Graphene Nanoribbons ◽

Label Free ◽

Magnetic Graphene ◽

Label Free Detection ◽

Impedimetric Immunosensor

Download Full-text

Impedimetric immunosensor based on gold nanoparticles modified graphene paper for label-free detection of Escherichia coli O157:H7

Biosensors and Bioelectronics ◽

10.1016/j.bios.2013.05.061 ◽

2013 ◽

Vol 49 ◽

pp. 492-498 ◽

Cited By ~ 127

Author(s):

Yixian Wang ◽

Jianfeng Ping ◽

Zunzhong Ye ◽

Jian Wu ◽

Yibin Ying

Keyword(s):

Escherichia Coli ◽

Gold Nanoparticles ◽

Escherichia Coli O157 ◽

Label Free ◽

Label Free Detection ◽

Graphene Paper ◽

Modified Graphene ◽

Impedimetric Immunosensor

Download Full-text

Sensitivity Improvement of an Impedimetric Immunosensor Using Functionalized Iron Oxide Nanoparticles

Journal of Sensors ◽

10.1155/2009/746548 ◽

2009 ◽

Vol 2009 ◽

pp. 1-12 ◽

Cited By ~ 9

Author(s):

Imen Hafaid ◽

Asma Gallouz ◽

Walid Mohamed Hassen ◽

Adnane Abdelghani ◽

Zina Sassi ◽

...

Keyword(s):

Iron Nanoparticles ◽

Gold Surface ◽

Antibody Immobilization ◽

Label Free ◽

Antibody Recognition ◽

Label Free Detection ◽

Measured Angle ◽

Sensitivity Improvement ◽

Antigen Antibody ◽

Impedimetric Immunosensor

This work has explored the development of impedimetric immunosensors based on magnetic iron nanoparticles (IrNP) functionalized with streptavidin to which a biotinylated FAB part of the antibody has been bound using a biotin-streptavidin interaction. SPR analysis shows a deviation on the measured (angle) during antigen-antibody recognition whereas label free detection using by EIS allows us to monitor variation of polarization resistance. Before detection, layers were analyzed by FTIR and AFM. Compared to immobilization of antibody on bare gold surface using aminodecanethiol SAM, antibody immobilization on nanoparticles permitted to reach lower detection limit: 500 pg/ml instead of 1 ng/ml to in the case of EIS and 300 ng/ml instead of 4.5 μg/ml in the case of SPR. Thus, it permitted to improve the sensitivity: from 257.3 Ω⋅cm2⋅μg−1⋅mlto 1871 Ω⋅cm2⋅μg−1⋅mlin the case of EIS and from0.003°μg−1⋅mlto0.094°μg−1⋅mlin the case of SPR.

Download Full-text