Biological role of glutathione in nitric oxide-induced toxicity in cell culture and animal models

2005 ◽  
Vol 39 (11) ◽  
pp. 1489-1498 ◽  
Author(s):  
C LI ◽  
T WRIGHT ◽  
M DONG ◽  
Y DOMMELS ◽  
L TRUDEL ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cell Culture ◽  
Animal Models ◽  
Biological Role

scholarly journals Investigation of the existence and biological role of l-arginine/nitric oxide pathway in human platelets by spin-trapping/EPR studies

1991 ◽  
Vol 202 (3) ◽  
pp. 923-930 ◽  
Author(s):  
Laszlo PRONAI ◽  
Kohji ICHIMORI ◽  
Hiroyuki NOZAKI ◽  
Hiroe NAKAZAWA ◽  
Haruka OKINO ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Human Platelets ◽  
Spin Trapping ◽  
Biological Role ◽  
Nitric Oxide Pathway

Mechanism and Biological Role of Nitric Oxide Binding to Cytochromec‘

Biochemistry ◽  
2002 ◽  
Vol 41 (39) ◽  
pp. 11582-11591 ◽  
Author(s):  
Anatoly L. Mayburd ◽  
Richard J. Kassner
Keyword(s):  
Nitric Oxide ◽  
Biological Role

O-GlcNAc transferase inhibitors: current tools and future challenges

2016 ◽  
Vol 44 (1) ◽  
pp. 88-93 ◽  
Author(s):  
Riccardo Trapannone ◽  
Karim Rafie ◽  
Daan M.F. van Aalten
Keyword(s):  
Animal Models ◽  
Biological Systems ◽  
Biological Role ◽  
Present Review ◽  
Biological Functions ◽  
Post Translational Modification ◽  
Embryonic Lethal ◽  
Future Challenges ◽  
Glcnac Transferase

The O-linked N-acetylglucosamine (O-GlcNAc) post-translational modification (O-GlcNAcylation) is the dynamic and reversible attachment of N-acetylglucosamine to serine and threonine residues of nucleocytoplasmic target proteins. It is abundant in metazoa, involving hundreds of proteins linked to a plethora of biological functions with implications in human diseases. The process is catalysed by two enzymes: O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) that add and remove sugar moieties respectively. OGT knockout is embryonic lethal in a range of animal models, hampering the study of the biological role of O-GlcNAc and the dissection of catalytic compared with non-catalytic roles of OGT. Therefore, selective and potent chemical tools are necessary to inhibit OGT activity in the context of biological systems. The present review focuses on the available OGT inhibitors and summarizes advantages, limitations and future challenges.

Role of nitric oxide in photoreceptor survival in embryonic chick retinal cell culture

1999 ◽  
Vol 55 (4) ◽  
pp. 423-431 ◽  
Author(s):  
Olivier Goureau ◽  
Fabienne Régnier-Ricard ◽  
Laurent Désiré ◽  
Yves Courtois
Keyword(s):  
Nitric Oxide ◽  
Cell Culture ◽  
Retinal Cell ◽  
Embryonic Chick

scholarly journals Cancer Chemopreventive Potential of Humulones and Isohumulones (Hops α- and Iso-α-acids): Induction of NAD(P)H:Quinone Reductase as a Novel Mechanism

2008 ◽  
Vol 3 (12) ◽  
pp. 1934578X0800301 ◽  
Author(s):  
Gregor Bohr ◽  
Karin Klimo ◽  
Josef Zapp ◽  
Hans Becker ◽  
Clarissa Gerhäuser
Keyword(s):  
Nitric Oxide ◽  
Cell Culture ◽  
Animal Models ◽  
Specific Activity ◽  
Oxidation Products ◽  
Phytochemical Analysis ◽  
Inos Activity ◽  
Raw264.7 Cell ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

Phytochemical analysis and chemopreventive testing of a special “α-/β-acid free” hops extract led to the identification of isohumulones (hops iso-α-acids) as potent inducers of NAD(P)H:quinone reductase (QR) activity. CD values (concentrations required to double the specific activity of QR in Hepa1c1c7 cell culture) were in the range of 1.3 to 10.2 μg/mL, with CD value of trans-isohumulone < cis-isoadhumulone < cis-isocohumulone < cis-isohumulone (+ trans-isoadhumulone). Humulones (hops α-acids) were equally active with CD values of 3.4 to 7.6 μg/mL. However, these activities were accompanied by cytotoxicity. Cohumulinone and humulinone, oxidation products of co- and n-humulone, were inactive. We further identified isohumulones as potent inhibitors of lipopolysaccharide-induced inducible nitric oxide synthase (iNOS) activity in Raw264.7 cell culture, with IC50 values of 5.9 – 18.4 μg/mL. Humulones and humulinones were inactive at concentrations < 20 μg/mL. These results indicate that isohumulones, which are considered as the most abundant class of polyphenols in beer, should by further investigated for chemopreventive efficacy in animal models.

c-Jun N-terminal kinase – c-Jun pathway transactivates Bim to promote osteoarthritis

2014 ◽  
Vol 92 (2) ◽  
pp. 132-139 ◽  
Author(s):  
Zhiqiang Ye ◽  
Yuxian Chen ◽  
Rongkai Zhang ◽  
Haitao Dai ◽  
Chun Zeng ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Articular Cartilage ◽  
Rna Interference ◽  
Animal Models ◽  
Chondrocyte Apoptosis ◽  
Kinase C ◽  
Tnf Α ◽  
Chemical Inhibitors ◽  
Joint Disorder

Osteoarthritis (OA) is a chronic degenerative joint disorder. Previous studies have shown abnormally increased apoptosis of chondrocytes in patients and animal models of OA. TNF-α and nitric oxide have been reported to induce chondrocyte ageing; however, the mechanism of chondrocyte apoptosis induced by IL-1β has remained unclear. The aim of this study is to identify the role of the c-Jun N-terminal kinase (JNK) – c-Jun pathway in regulating induction of Bim, and its implication in chondrocyte apoptosis. This study showed that Bim is upregulated in chondrocytes obtained from the articular cartilage of OA patients and in cultured mouse chondrocytes treated with IL-1β. Upregulation of Bim was found to be critical for chondrocyte apoptosis induced by IL-1β, as revealed by the genetic knockdown of Bim, wherein apoptosis was greatly reduced in the chondrocytes. Moreover, activation of the JNK–c-Jun pathway was observed under IL-1β treatment, as indicated by the increased expression levels of c-Jun protein. Suppression of the JNK–c-Jun pathway, using chemical inhibitors and RNA interference, inhibited the Bim upregulation induced by IL-1β. These findings suggest that the JNK–c-Jun pathway is involved in the upregulation of Bim during OA and that the JNK–c-Jun–Bim pathway is vital for chondrocyte apoptosis.

The biological role of nitric oxide in bacteria

1993 ◽  
Vol 160 (4) ◽  
pp. 253-264 ◽  
Author(s):  
Walter G. Zumft
Keyword(s):  
Nitric Oxide ◽  
Biological Role

scholarly journals Role of Pomegranate in the Management of Cancer

2021 ◽  
Author(s):  
Amulya Thotambailu ◽  
Deepu Cheriamane ◽  
Manjula Santhepete ◽  
Satheesh Kumar Bhandary ◽  
Jiju Avanippully ◽  
...  
Keyword(s):  
Cell Culture ◽  
Animal Models ◽  
Head And Neck ◽  
Folk Medicine ◽  
Cellular Transformation ◽  
Punica Granatum ◽  
Lung Cancers ◽  
Culture Systems ◽  
Cell Culture Systems

Pomegranate (Punica granatum) has been used since ages as a folk medicine. Studies have shown that pomegranate extracts have a role in various signaling pathways involved in inflammation, cellular transformation, hyperproliferation, angiogenesis, initiation of tumorigenesis, and eventually suppressing the final steps of tumorigenesis and metastasis. In this chapter, we have discussed some of the polyphenolic constituents present in pomegranate and their medical value, and we then discussed studies on chemopreventive/chemotherapeutic properties of pomegranate against various types of cancer, such as skin, prostate, colon, head and neck and lung cancers in cell culture systems, animal models and humans.

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