Free radical scavenging, anti-inflammatory activity and phytochemical constituents of Ficus talboti King bark

2012 ◽  
Vol 53 ◽  
pp. S107-S108 ◽  
Author(s):  
K. Arunachalam⁎ ◽  
T. Parimelazhgan et al
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Inflammatory Activity ◽  
Phytochemical Constituents ◽  
Anti Inflammatory ◽  
Ficus Talboti

scholarly journals In Vitro and in Vivo Evaluation Of Pharmacological Activites of Pouzolzia Sanguinea

2021 ◽  
Vol 29 (2) ◽  
pp. 31-42
Author(s):  
MQ Ahsan ◽  
MT Alam ◽  
MMU Chowdhury ◽  
MT Nasim ◽  
SMS Islam
Keyword(s):  
Body Weight ◽  
Free Radical ◽  
Protein Denaturation ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Standard Drug ◽  
Phytochemical Constituents ◽  
Anti Inflammatory

Pouzolzia sanguinea grows in tropical and sub-tropical regions of Bangladesh, used for a variety of purposes including pain, rheumatoid kidney diseases in traditional medicine. The crude ethanolic leaf extract of P. sanguinea with its different fractions (ethanol, n-hexane, and chloroform) was investigated for phytochemical constituents, in vitro antioxidant, anti-inflammatory effects, in-vivo analgesic and antipyretic activities. Preliminary phytochemical constituents were identified by chemical group test. P. sanguinea fractionated extracts contain alkaloids, glycosides, tannins, flavonoids, saponins, gums, and amides. Antioxidant activity test was performed by both qualitative (TLC and Rf value) and quantitative tests (inhibition of DPPH free radical scavenging). Extracts exhibited significant (p <0.001, p <0.0001) inhibition of DPPH free radical scavenging activity as compared to the standard drug ascorbic acid at similar doses. In vitro anti-inflammatory activity was determined by protein denaturation of egg albumin method. The percent inhibition of protein denaturation in the experiment of ethanol extract was found significantly higher (p <0.0001) compared with chloroform and n-hexane extracts. In addition, in vivo analgesic and antipyretic effects were determined in mice by acetic acid-induced writhing and yeast-induced pyrexia methods. The ethanol extracts of P. sanguinea exhibited inhibition of writhing reflex on mice by 71.58% at the dose of 500 mg/kg body weight which had greater analgesic activity than other n-hexane and chloroform extracts. In the anti-pyretic test, fractional extracts ethanol, chloroform, and n-hexane at a dose of 500 mg/kg body weight significantly (p <0.05) decreased pyrexia in mice up to 3 h as compared with the positive control paracetamol drug at a dose of 150 mg/kg body weight. In our in vitro and in vivo study models, it is evident that Pouzolzia sanguinea fractionated extracts showed significant antioxidant, anti-inflammatory, analgesic, and antipyretic activities. J. Bio-Sci. 29(2): 31-42, 2021 (December)

A Preliminary Study on In Vitro Antioxidant and In Vivo Anti-Inflammatory Activity of Cissus quadrangularis Linn.

2021 ◽  
pp. 2619-2624
Author(s):  
Mamta Tiwari ◽  
Pushpraj S Gupta ◽  
Nisha Sharma
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Inflammatory Activity ◽  
Scavenging Activity ◽  
Cissus Quadrangularis ◽  
Anti Inflammatory

Cissus quadrangularis L. is a member of succulent climber of family Vitaceae and it is also called as bone setter because of its bone healing property. The present study focused to assess the in vitro antioxidant and in vivo anti-inflammatory activity of the plant stem extracts in different solvents namely petroleum ether, chloroform, ethyl acetate and methanol. Among all these extracts; ethyl extract showed highest phenolic and flavonoid content (19.35±0.29mg GA/g, 8.68±0.06 RE/g dry wt. respectively). In DPPH free radical scavenging assay all mentioned extracts exhibited free radical scavenging activity but the highest free radical scavenging activity was showed by ethyl acetate extract in increasing dose dependent manner. On the basis of highest phenolic, flavonoid content and free radical scavenging activity ethyl acetate extract was selected for anti- inflammatory activity. The anti-inflammatory activity of Cissus quadrangularis Lin. was investigated in carageenan induced inflammation in Wistar albino rats. Two different doses 250mg/kg and 500mg/kg were administered to the animals. The results showed that both doses had significant reduction in inflammatory condition but dose 500mg/kg showed maximum inflammatory reduction on comparing with control and standard treated groups.

Anti-Inflammatory Activity of Human Lens Crystallin Derived Peptide

2021 ◽  
Vol 18 ◽  
Author(s):  
Anuraag Muralidharan ◽  
Tenzin Tender ◽  
Pallavi K Shetty ◽  
Srinivas Mutalik ◽  
Krishna Sharma K ◽  
...  
Keyword(s):  
Free Radical ◽  
Skin Permeation ◽  
Diclofenac Sodium ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Inflammatory Activity ◽  
Human Lens ◽  
Permeation Studies ◽  
Anti Inflammatory

Background: Inflammation has become the culmination point for several chronic diseases like skin diseases, asthma, neurological disorders, cancer and cardiovascular disorders. Mini αA-crystallin peptide, identified from a highly conserved region of human lens protein αA-crystallin, is known to have a chaperone-like function, hence has generated interest in exploring the anti-inflammatory potential of the peptide. Objective: The objective of the study was to evaluate anti-inflammatory potential of mini αA chaperone using in vitro, ex-vivo and in vivo models. Methods: The peptide was tested for its phosphodiestarase4 B inhibition, anti-inflammatory and free radical scavenging abilities in HaCat cells. Carbopol gel formulations with varying concentrations of mini αA-crystallin peptide and diclofenac sodium were prepared and optimized. Skin permeation studies of prepared formulations were carried out on excised abdominal skin of Wistar rat using a vertical type diffusion cell. Carrageenan induced rat paw oedema model was used for determining the anti-inflammatory potential of the peptide in prepared gel formulation with or without diclofenac sodium. Results: The peptide exhibited appreciable free radical scavenging and weak PDE4B inhibition. Gel formulation with 1% Tween-80, 1% carbopol and 10% ethanol showed better permeation compared to other formulations. The in vitro skin permeation studies revealed good improvement in permeation characteristics of diclofenac and peptide from the gels. The peptide was retained within the skin tissue, which is an ideal requirement for the delivery of anti-inflammatory topical formulation. In preclinical anti-inflammatory studies, gel formulation containing mini αA-peptide and diclofenac sodium showed a significant decrease in paw volume compared to other combinations tested. Conclusion: The study revealed the additive effect in anti-inflammatory activity by combining mini-αA peptide and diclofenac sodium which effectively reduced the inflammation.

scholarly journals Antioxidant, anti-inflammatory and anti-hyperglycemic activity of aqueous and methanolic extract of Houttuynia cordata: an in vitro and in vivo study

2021 ◽  
Vol 16 (3) ◽  
pp. 145-154
Author(s):  
Careen Liza Pakyntein ◽  
Donkupar Syiem ◽  
Daiahun Thabah ◽  
Shelareen Ediemi Sunn
Keyword(s):  
Free Radical ◽  
Side Effects ◽  
Methanolic Extract ◽  
Protein Denaturation ◽  
Colorimetric Method ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Inflammatory Activity ◽  
Houttuynia Cordata ◽  
Anti Inflammatory

Available synthetic antioxidants such as butylated hydroxyl anisole, butylated hydroxyl toluene, propyl gallate, and ascorbic acid exhibit several side effects. To curb these side effects, more effective, less toxic, and cost-effective drugs are required. Therefore, this study aims to screen and evaluate the antioxidant as well as the anti-inflammatory and antidiabetic potential of Houttuynia cordata collected from Mairang village, West Khasi Hills, Meghalaya, India using several standard methods. The aqueous and methanolic extracts of H. cordata were evaluated by screening their ability to scavenge 1,1-diphenyl-2-dipicrylhydrozyl, 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid), hydrogen peroxide, and nitric oxide. Total phenol and flavonoid content was measured by Folin-Ciocalteau and by AlCl3 colorimetric method respectively. The anti-inflammatory activity of the plant was determined using the protein denaturation method. Methanolic and aqueous extracts of Houttuynia cordata exhibit varying free radical scavenging and anti-inflammatory activity. Among the extracts used in the study, the methanolic extract of Houttuynia cordata elicited a higher activity than the aqueous extract. Houttuynia cordata also elicited a marked reduction in blood glucose level of normal and alloxan-induced diabetic mice. Flavonoids, which have been reported to possess anti-inflammatory, enzyme inhibition, antimicrobial, anticancer, antiallergy, and antioxidant properties may contribute towards the free radical scavenging and anti-inflammatory effect of Houttuynia cordata.

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