Molecular cloning and characterization of a novel c-type lysozyme gene in swimming crab Portunus trituberculatus

2010 ◽  
Vol 29 (2) ◽  
pp. 286-292 ◽  
Author(s):  
Luqing Pan ◽  
Feng Yue ◽  
Jingjing Miao ◽  
Lin Zhang ◽  
Jian Li
Keyword(s):  
Molecular Cloning ◽  
Portunus Trituberculatus ◽  
Swimming Crab ◽  
Lysozyme Gene

Molecular cloning and characterization of a heat shock protein 70 gene in swimming crab (Portunus trituberculatus)

2010 ◽  
Vol 28 (1) ◽  
pp. 56-64 ◽  
Author(s):  
Zhaoxia Cui ◽  
Yuan Liu ◽  
Weisha Luan ◽  
Qianqian Li ◽  
Danhua Wu ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Molecular Cloning ◽  
Heat Shock Protein 70 ◽  
Portunus Trituberculatus ◽  
Swimming Crab

Molecular cloning and characterization of prophenoloxidase gene in swimming crab Portunus trituberculatus

2010 ◽  
Vol 28 (1) ◽  
pp. 106-112 ◽  
Author(s):  
Ping Chen ◽  
Jitao Li ◽  
Jian Li ◽  
Ping Liu ◽  
Baoquan Gao ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Portunus Trituberculatus ◽  
Swimming Crab

Molecular cloning, tissue distribution and gene expression in response to nutritional regulation of sterol regulatory element binding protein-1 from the swimming crab Portunus trituberculatus (Miers, 1876) (Decapoda, Portunidae)

Crustaceana ◽  
2021 ◽  
Vol 94 (2) ◽  
pp. 235-250
Author(s):  
Xiaoying Hu ◽  
Bo Shi ◽  
Min Jin ◽  
Xuexi Wang ◽  
Ye Yuan ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Regulatory Element ◽  
Basal Diet ◽  
Tissue Expression ◽  
Portunus Trituberculatus ◽  
Pcr Analysis ◽  
Dietary Iron ◽  
Nutritional Regulation ◽  
Swimming Crab ◽  
Sterol Regulatory Element

Abstract The sterol regulatory element-binding proteins (SREBPs) are a family of transcription factors known to activate the transcription of genes encoding key lipogenic enzymes. The present study reports on the molecular cloning, tissue expression and nutritional regulation of SREBP-1 from the swimming crab Portunus trituberculatus (Miers, 1876). The SREBP-1 full-length cDNA was 3785 bp, encoding a polypeptide of 1039 amino acids. Quantitative PCR analysis revealed that SREBP-1 expression was detected in various tissues, and the significantly higher expression levels were found in eyestalk and hepatopancreas compared with other tested tissues. Additionally, the effects of dietary iron on expression of SREBP-1 were investigated, and the results indicated that SREBP-1 expressions were down-regulated by crabs fed diets containing 218.9 and 373.9 mg/kg iron compared with that fed the basal diet (55.2 mg/kg). Suggesting that the relative expression level of SREBP-1 can be suppressed by dietary iron supplementation. These findings provide further insight into the regulatory capacity of SREBP-1 in the lipid anabolism of P. trituberculatus.

Molecular cloning and characterization of c-type lysozyme gene in orange-spotted grouper, Epinephelus coioides

2012 ◽  
Vol 33 (2) ◽  
pp. 186-196 ◽  
Author(s):  
Shina Wei ◽  
Youhua Huang ◽  
Jia Cai ◽  
Xiaohong Huang ◽  
Jing Fu ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Epinephelus Coioides ◽  
Lysozyme Gene
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