scholarly journals Transglutaminase 1 and 2 are localized in different blood cells in the freshwater crayfish Pacifastacus leniusculus

2020 ◽  
Vol 104 ◽  
pp. 83-91
Author(s):  
Kingkamon Junkunlo ◽  
Kenneth Söderhäll ◽  
Irene Söderhäll
Keyword(s):  
Blood Cells ◽  
Pacifastacus Leniusculus ◽  
Freshwater Crayfish ◽  
Transglutaminase 1

PHYSIOLOGICAL RESPONSES OF THE CRAYFISH PACIFASTACUS LENIUSCULUS TO ENVIRONMENTAL HYPEROXIA: I. EXTRACELLULAR ACID-BASE AND ELECTROLYTE STATUS AND TRANSBRANCHIAL EXCHANGE

1989 ◽  
Vol 143 (1) ◽  
pp. 33-51 ◽  
Author(s):  
MICHÉLE G. WHEATLY
Keyword(s):  
Time Course ◽  
Extracellular Fluid ◽  
Respiratory Acidosis ◽  
Pacifastacus Leniusculus ◽  
Initial Increase ◽  
Flux Analysis ◽  
Freshwater Crayfish ◽  
Acid Base ◽  
Sequence Of Events ◽  
Fluid Compartment

Extracellular acid--base and ionic status, and transbranchial exchange of acidic equivalents and electrolytes, were monitored in freshwater crayfish (Pacifastacus leniusculus) during control normoxia (PO2 = 148 mmHg; 1 mmHg = 133.3 Pa), 72 h of hyperoxia (PO2 = 500 mmHg) and 24 h of recovery. An initial (3 h) respiratory acidosis of 0.2 pH units was completely compensated within 48 h by a 50% increase in metabolic [HCO3−+CO32-] accompanied by a significant reduction in circulating [Cl−]. In addition, the original increase in Pco2 was partially accommodated. The time course of transbranchial acidic equivalent exchange paralleled the change in extracellular metabolic base load with a significant branchial output of H+ during the first 48 h of hyperoxia. This was associated with net branchial effluxes of Cl− and Mg2+. Unidirectional flux analysis revealed parallel reductions in Na+ influx and efflux during initial hyperoxic exposure, reflecting an alteration in exchange diffusion. The net Cl− efflux was due to an initial increase in efflux followed by a reduction in influx. The reverse sequence of events occurred more rapidly when normoxia was reinstated: metabolic base was removed from the haemolymph and control haemolymph acid--base and ion levels were re-established within 24 h. Transbranchial fluxes of acidic equivalents similarly recovered within 24 h although net Na+ output and Cl− uptake persisted. The study attempted to identify relationships between branchial net H+ exchange and components of Na+ and Cl− exchange and quantitatively to correlate changes in the acidic equivalent and electrolyte concentrations in the extracellular fluid compartment with those in the external water.

Effects of thermic manipulation throughout egg development on the reproductive efficiency of the freshwater crayfish (Pacifastacus leniusculus Dana)

Aquaculture ◽  
1988 ◽  
Vol 72 (3-4) ◽  
pp. 341-348 ◽  
Author(s):  
Jesus D. Celada ◽  
Jose M. Carral ◽  
Vicente R. Gaudioso ◽  
Consuelo Temino ◽  
Ramon Fernandez
Keyword(s):  
Pacifastacus Leniusculus ◽  
Reproductive Efficiency ◽  
Freshwater Crayfish ◽  
Egg Development

Accumulation and toxicity of aluminium-contaminated food in the freshwater crayfish, Pacifastacus leniusculus

2011 ◽  
Vol 105 (3-4) ◽  
pp. 535-542 ◽  
Author(s):  
Katie Woodburn ◽  
Rachel Walton ◽  
Catherine McCrohan ◽  
Keith White
Keyword(s):  
Pacifastacus Leniusculus ◽  
Freshwater Crayfish ◽  
Contaminated Food

scholarly journals A gC1qR Prevents White Spot Syndrome Virus Replication in the Freshwater Crayfish Pacifastacus leniusculus

2010 ◽  
Vol 84 (20) ◽  
pp. 10844-10851 ◽  
Author(s):  
Apiruck Watthanasurorot ◽  
Pikul Jiravanichpaisal ◽  
Irene Söderhäll ◽  
Kenneth Söderhäll
Keyword(s):  
Cell Culture ◽  
Viral Replication ◽  
White Spot Syndrome Virus ◽  
Pacifastacus Leniusculus ◽  
White Spot ◽  
Tissue Cell ◽  
Freshwater Crayfish ◽  
White Spot Syndrome

ABSTRACT The gC1qR/p32 protein is a multiple receptor for several proteins and pathogens. We cloned a gC1qR homologue in a crustacean, Pacifastacus leniusculus, and analyzed the expression of P. leniusculus C1qR (PlgC1qR) in various tissues. The gC1qR/p32 transcript was significantly enhanced by white spot syndrome virus (WSSV) infection 6 h after viral infection both in vitro in a hematopoietic tissue cell culture (Hpt) and in vivo compared to appropriate controls. Moreover, PlgC1qR silencing in both the Hpt cell culture and live crayfish enhanced the WSSV replication. In addition, by making a recombinant PlgC1qR protein we could show that if this recombinant protein was injected in a crayfish, Pacifastacus leniusculus, followed by injection of WSSV, this significantly reduced viral replication in vivo. Furthermore, if the recombinant PlgC1qR was incubated with Hpt cells and then WSSV was added, this also reduced viral replication. These experiments clearly demonstrate that recombinant PlgC1qR reduce WSSV replication both in vivo and in vitro. The results from a far-Western overlay and glutathione S-transferase pull-down assays showed that PlgC1qR could bind to VP15, VP26, and VP28. Altogether, these results demonstrate a role for PlgC1qR in antiviral activity against WSSV.

scholarly journals Phenoloxidase Is an Important Component of the Defense against Aeromonas hydrophila Infection in a Crustacean, Pacifastacus leniusculus

2007 ◽  
Vol 282 (46) ◽  
pp. 33593-33598 ◽  
Author(s):  
Haipeng Liu ◽  
Pikul Jiravanichpaisal ◽  
Lage Cerenius ◽  
Bok Luel Lee ◽  
Irene Söderhäll ◽  
...  
Keyword(s):  
Rna Interference ◽  
Bacterial Growth ◽  
Aeromonas Hydrophila ◽  
Immune Defense ◽  
Pacifastacus Leniusculus ◽  
Pathogenic Bacterium ◽  
Nodule Formation ◽  
Freshwater Crayfish ◽  
Phenoloxidase Activity ◽  
Microbial Infections

The melanization cascade, in which phenoloxidase is the terminal enzyme, appears to play a key role in recognition of and defense against microbial infections in invertebrates. Here, we show that phenoloxidase activity and melanization are important for the immune defense toward a highly pathogenic bacterium, Aeromonas hydrophila, in the freshwater crayfish, Pacifastacus leniusculus. RNA interference-mediated depletion of crayfish prophenoloxidase leads to increased bacterial growth, lower phagocytosis, lower phenoloxidase activity, lower nodule formation, and higher mortality when infected with this bacterium. In contrast, if RNA interference of pacifastin, an inhibitor of the crayfish prophenoloxidase activation cascade, is performed, it results in lower bacterial growth, increased phagocytosis, increased nodule formation, higher phenoloxidase activity, and delayed mortality. Our data therefore suggest that phenoloxidase is required in crayfish defense against an infection by A. hydrophila, a highly virulent and pathogenic bacterium to crayfish.

COMPARATIVE STUDIES OF CAROTENOIDS IN FOUR SPECIES OF CRAYFISH

Crustaceana ◽  
1999 ◽  
Vol 72 (7) ◽  
pp. 693-700 ◽  
Author(s):  
◽  
Keyword(s):  
Thin Layer ◽  
Comparative Studies ◽  
Pacifastacus Leniusculus ◽  
Carotenoid Content ◽  
Freshwater Crayfish ◽  
Astacus Astacus ◽  
Astacus Leptodactylus ◽  
Orconectes Limosus ◽  
Layer Chromatography ◽  
Crayfish Species

AbstractUsing column and thin-layer chromatography, the authors investigated the presence of carotenoids in the carapaces and muscles of freshwater crayfish species. Fifteen carotenoids were found, among which canthaxanthin, adonixanthin, and astaxanthin were predominant. The highest carotenoid content was revealed in the carapaces of Pacifastacus leniusculus and Astacus astacus and in the muscles of Astacus leptodactylus and Orconectes limosus. En utilisant la chromatographie sur colonnes et en couches minces, les auteurs ont recherche la presence de carotenoodes dans les carapaces et les muscles de plusieurs especes d'ecrevisses. Quinze carotenoodes ont ete trouves, parmi lesquels la canthaxanthine, l'adonixanthine et l'asthaxantine etaient dominantes. Le contenu en carotenoodes le pluseleve a ete trouve dans les carapaces de Pacifastacus leniusculus et Astacus astacus et dans les muscles de Astacus leptodactylus et Orconectes limosus.

Characterization of two crustin antimicrobial peptides from the freshwater crayfish Pacifastacus leniusculus

2010 ◽  
Vol 104 (3) ◽  
pp. 234-238 ◽  
Author(s):  
Suchao Donpudsa ◽  
Vichien Rimphanitchayakit ◽  
Anchalee Tassanakajon ◽  
Irene Söderhäll ◽  
Kenneth Söderhäll
Keyword(s):  
Antimicrobial Peptides ◽  
Pacifastacus Leniusculus ◽  
Freshwater Crayfish

scholarly journals SI on economic costs of invasions - Feeling the pinch: global economic costs of crayfish invasions and comparison with other aquatic crustaceans

2021 ◽  
Author(s):  
Antonin Kouba ◽  
Francisco J. Oficialdegui ◽  
Ross N. Cuthbert ◽  
Melina Kourantidou ◽  
Josie South ◽  
...  
Keyword(s):  
North America ◽  
Multiple Scales ◽  
Pacifastacus Leniusculus ◽  
Freshwater Crayfish ◽  
Similar Magnitude ◽  
Economic Costs ◽  
Knowledge Gaps ◽  
Signal Crayfish ◽  
Invasive Crayfish ◽  
European Economies

Abstract Despite voluminous literature identifying invasive species impacts, understandings of monetary costs remain limited. Recently, profound impacts have been attributed to invasive crustaceans, but associated monetary costs lack synthesis. Here, we analyse globally reported costs of invasive freshwater crayfish across taxonomic, spatial and temporal descriptors. Moreover, we compare their cost magnitude to other invasive crustaceans — crabs, amphipods and lobsters. Between 2000 and 2020, crayfish caused US$ 1.28 billion in reported costs; the vast majority (95%) attributed to Astacidae (principally the signal crayfish Pacifastacus leniusculus) and the remainder to Cambaridae. According to reports, crayfish costs mostly impacted European economies (US$ 1.23 billion), followed by costs reported for North America and Asia. Despite well-known damages caused by invasive crayfish, costs were unreported elsewhere, highlighting knowledge gaps and challenges in cost quantifications. Invasive crayfish costs increased exponentially in the last two decades, averaging at US$ 61 million per-annum. Invasive crabs caused costs of similar magnitude (US$ 1.25 billion; US$ 53 million per-annum) but were mostly confined to North America (95%). Damage-related costs dominated for both crayfish (83%) and crabs (99%), with management spending lacking. Reported economic impacts from amphipods (US$ 178.8 thousand) and lobsters (US$ 44.6 thousand) were considerably lower. We identify burgeoning economic costs from these invasive groups yet highlight pervasive knowledge gaps at multiple scales. Further cost reporting is required to better-ascertain the true scale of monetary costs caused by invasive aquatic crustaceans.

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