Application of Short Tandem Repeat markers in diagnosis of chromosomal aneuploidies and forensic DNA investigation in Pakistan

Gene ◽  
2014 ◽  
Vol 548 (2) ◽  
pp. 217-222 ◽  
Author(s):  
Hafsah Muhammad Chishti ◽  
Muhammad Ansar ◽  
Muhammad Ajmal ◽  
Abdul Hameed
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Forensic Dna ◽  
Short Tandem Repeat Markers ◽  
Chromosomal Aneuploidies ◽  
Short Tandem

Elimination of contaminating amplified short tandem repeat products by autoclaving and ultraviolet irradiation

2017 ◽  
Vol 58 (1) ◽  
pp. 25-31 ◽  
Author(s):  
Jun Ohta ◽  
Atsunori Tanaka
Keyword(s):  
Tandem Repeat ◽  
Ultraviolet Irradiation ◽  
Short Tandem Repeat ◽  
Polymerase Chain Reaction Amplification ◽  
Dna Typing ◽  
Forensic Dna ◽  
Probative Value ◽  
Reaction Amplification ◽  
Dna Template ◽  
Short Tandem

DNA contamination can result in false interpretation of short tandem repeat (STR) DNA typing. Proper decontamination is particularly required in forensic DNA laboratories where probative value of the evidence may be affected. The aim of this study was to establish an effective DNA decontamination procedure for amplified STR products focusing on laboratory-related contamination. We verified the effectiveness of thermally and temporally extended autoclaving and ultraviolet irradiation for the elimination of contaminating amplified STR products. STR amplification products were prepared using a control genomic DNA template and generated using the AmpFℓSTR® Identifiler® Plus and Yfiler® polymerase chain reaction amplification kits. In this study, the contaminants were dried before decontamination treatment, which resembles actual contamination situations. One microlitre of amplified STR products was eliminated by a combination of autoclaving (128°C, 420 min) and UV irradiation (60 J/cm2). Our results reveal that the combination treatment represents an effective DNA decontamination procedure and a practicable method in standard level laboratories. Finally, we propose a comprehensive approach for forensic DNA laboratories to implement to minimise contamination issues and guarantee provision of authentic results.

scholarly journals Genome Wide Characterization of Short Tandem Repeat Markers in Sweet Orange (Citrus sinensis)

PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e104182 ◽  
Author(s):  
Manosh Kumar Biswas ◽  
Qiang Xu ◽  
Christoph Mayer ◽  
Xiuxin Deng
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Citrus Sinensis ◽  
Sweet Orange ◽  
Genome Wide ◽  
Short Tandem Repeat Markers ◽  
Short Tandem
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