Heterologous expression of betaine aldehyde dehydrogenase gene from Ammopiptanthus nanus confers high salt and heat tolerance to Escherichia coli

Gene ◽  
2014 ◽  
Vol 549 (1) ◽  
pp. 77-84 ◽  
Author(s):  
Hao-Qiang Yu ◽  
Ying-Ge Wang ◽  
Tai-Ming Yong ◽  
Yue-Hui She ◽  
Feng-Ling Fu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Heterologous Expression ◽  
Aldehyde Dehydrogenase ◽  
Heat Tolerance ◽  
High Salt ◽  
Betaine Aldehyde Dehydrogenase ◽  
Betaine Aldehyde ◽  
Ammopiptanthus Nanus ◽  
Dehydrogenase Gene

Optimization of the Heterologous Expression of Sesuvium portulacastrum Betaine Aldehyde Dehydrogenase in Escherichia coli

2013 ◽  
Vol 864-867 ◽  
pp. 221-224
Author(s):  
Cheng Long Yang ◽  
Rui Mei Li ◽  
Yang Zhou ◽  
Rui Jun Duan ◽  
Shao Ping Fu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fusion Protein ◽  
Aldehyde Dehydrogenase ◽  
Induction Time ◽  
Expression System ◽  
Betaine Aldehyde Dehydrogenase ◽  
Sesuvium Portulacastrum ◽  
Betaine Aldehyde ◽  
Dehydrogenase Gene ◽  
Full Length Sequence

A full-length sequence coding for a betaine aldehyde dehydrogenase gene from S. portulacastrum was cloned into expression vector pGEX-4T-1, and named pGEX-4T-SpBADH. The GST-SpBADH fusion protein was expressed and the expression conditions were optimized. Through the research on optimization of expression the concentration of IPTG, concentration of bacterium, induction time and temperature and so on, the results showed, the expression of GST-SpBADH increased accompany with the induction time. The expression level of GST-SpBADH fusion protein reached the highest for 5 h cultured and for OD600 is about 0.6 at 37°C, 0.2 mmol/L IPTG can effectively induce the expression of GST-SpBADH in Escherichia coli expression system.

Betaine aldehyde dehydrogenase from Pseudomonas aeruginosa: cloning, over-expression in Escherichia coli, and regulation by choline and salt

2005 ◽  
Vol 185 (1) ◽  
pp. 14-22 ◽  
Author(s):  
Roberto Velasco-García ◽  
Miguel Angel Villalobos ◽  
Miguel A. Ramírez-Romero ◽  
Carlos Mújica-Jiménez ◽  
Gabriel Iturriaga ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Aldehyde Dehydrogenase ◽  
Betaine Aldehyde Dehydrogenase ◽  
Over Expression ◽  
Betaine Aldehyde ◽  
Expression In Escherichia Coli
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