Molecular approach for analysis of in situ feeding by the dinoflagellate Noctiluca scintillans

Harmful Algae ◽  
2020 ◽  
Vol 99 ◽  
pp. 101928
Author(s):  
Goh Nishitani ◽  
Masaomi Shiromoto ◽  
Waka Sato-Okoshi ◽  
Akira Ishikawa
2021 ◽  
Author(s):  
Joe Premier ◽  
Martin Gahbauer ◽  
Franz Leibl ◽  
Marco Heurich

2014 ◽  
Vol 5 ◽  
pp. 983-993 ◽  
Author(s):  
Marcelo Rodrigues ◽  
Birgit Lengerer ◽  
Thomas Ostermann ◽  
Peter Ladurner

The use of molecular biology tools in the field of bioadhesion is still in its infancy. For new research groups who are considering taking a molecular approach, the techniques presented here are essential to unravelling the sequence of a gene, its expression and its biological function. Here we provide an outline for addressing adhesion-related genes in diverse organisms. We show how to gradually narrow down the number of candidate transcripts that are involved in adhesion by (1) generating a transcriptome and a differentially expressed cDNA list enriched for adhesion-related transcripts, (2) setting up a BLAST search facility, (3) perform an in situ hybridization screen, and (4) functional analyses of selected genes by using RNA interference knock-down. Furthermore, latest developments in genome-editing are presented as new tools to study gene function. By using this iterative multi-technologies approach, the identification, isolation, expression and function of adhesion-related genes can be studied in most organisms. These tools will improve our understanding of the diversity of molecules used for adhesion in different organisms and these findings will help to develop innovative bio-inspired adhesives.


Zootaxa ◽  
2020 ◽  
Vol 4766 (2) ◽  
pp. 201-260 ◽  
Author(s):  
CHRISTOPHER L. MAH

Exploratory cruises by the NOAA Ship Okeanos Explorer have resulted in a substantial contribution in our understanding of deep-sea echinoderm biodiversity, biology, and ecology in the North Atlantic. This includes the description and in situ feeding observations of two, new corallivorous goniasterid species, Evoplosoma nizinskiae n. sp. and Sibogaster bathyheuretor n. sp. Significant in situ observations include a synchronous feeding event including multiple goniasterid asteroids and a cidaroid urchin on a large demosponge, providing new data for understanding echinoderm feeding behavior, including agonistic behavior, in deep-sea settings and new, in situ feeding observations for 28 deep-sea species including the myxasterid Pythonaster atlantidis, the korethrasterid Remaster palmatus and the poorly understood hippasterine goniasterids, Gilbertaster caribaea and Sthenaster emmae. 


2011 ◽  
Vol 409 (15) ◽  
pp. 2874-2880 ◽  
Author(s):  
Maria del Mar Alguacil ◽  
Emma Torrecillas ◽  
Josef Kohler ◽  
Antonio Roldán

2007 ◽  
Vol 52 (1) ◽  
pp. 428-440 ◽  
Author(s):  
Gitai Yahel ◽  
Frank Whitney ◽  
Henry M. Reiswig ◽  
Dafne I. Eerkes-Medrano ◽  
Sally P. Leys

2019 ◽  
Vol 167 ◽  
pp. 115106
Author(s):  
Natàlia Blázquez-Pallí ◽  
Mònica Rosell ◽  
Joan Varias ◽  
Marçal Bosch ◽  
Albert Soler ◽  
...  

2020 ◽  
Vol 112 ◽  
pp. 106123 ◽  
Author(s):  
Junting Song ◽  
Hongsheng Bi ◽  
Zhonghua Cai ◽  
Xuemin Cheng ◽  
Yonghong He ◽  
...  

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